The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.
Characterization of the xylose-transporting properties of yeast hexose transporters and their influence on xylose utilization
Abstract
For an economically feasible production of ethanol from plant biomass by microbial cells, the fermentation of xylose is important. As xylose uptake might be a limiting step for xylose fermentation by recombinant xylose-utilizing Saccharomyces cerevisiae cells a study of xylose uptake was performed. After deletion of all of the 18 hexose-transporter genes, the ability of the cells to take up and to grow on xylose was lost. Reintroduction of individual hexose-transporter genes in this strain revealed that at intermediate xylose concentrations the yeast high- and intermediate-affinity transporters Hxt4, Hxt5, Hxt7 and Gal2 are important xylose-transporting proteins. Several heterologous monosaccharide transporters from bacteria and plant cells did not confer sufficient uptake activity to restore growth on xylose. Overexpression of the xylose-transporting proteins in a xylose-utilizing PUA yeast strain did not result in faster growth on xylose under aerobic conditions nor did it enhance the xylose fermentation rate under anaerobic conditions. The results of this study suggest that xylose uptake does not determine the xylose flux under the conditions and in the yeast strains investigated.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Molecular cloning: A laboratory manual
Deininger, P. (1990) · Analytical Biochemistry · doi:10.1016/0003-2697(90)90595-z · Cited by 84,682
Mumberg, D., Muller, R., & Funk, M. (1994) · Nucleic Acids Research · doi:10.1093/nar/22.25.5767 · Cited by 955
Function and Regulation of Yeast Hexose Transporters
Özcan, S., & Johnston, M. (1999) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.63.3.554-569.1999 · Cited by 720
Wieczorke, R., Krampe, S., Weierstall, T., Freidel, K., Hollenberg, C. P., & Boles, E. (1999) · FEBS Letters · doi:10.1016/s0014-5793(99)01698-1 · Cited by 635
Self-citationXylose fermentation by Saccharomyces cerevisiae
K�tter, P., & Ciriacy, M. (1993) · Applied Microbiology and Biotechnology · doi:10.1007/bf00167144 · Cited by 509
Complementation of Saccharomyces cerevisiae auxotrophic mutants by Arabidopsis thaliana cDNAs
Minet, M., Dufour, M.-E., & Lacroute, F. (1992) · The Plant Journal · doi:10.1111/j.1365-313x.1992.00417.x · Cited by 493
The molecular genetics of hexose transport in yeasts
Boles, E. (1997) · FEMS Microbiology Reviews · doi:10.1016/s0168-6445(97)00052-1 · Cited by 429
Self-citationComplementation of Saccharomyces cerevisiae auxotrophic mutants by Arabidopsis thaliana cDNAs
Minet, M., Dufour, M., & Lacroute, F. (1992) · The Plant Journal · doi:10.1046/j.1365-313x.1992.t01-38-00999.x · Cited by 395
Eliasson, A., Christensson, C., Wahlbom, C. F., & Hahn-Hägerdal, B. (2000) · Applied and Environmental Microbiology · doi:10.1128/aem.66.8.3381-3386.2000 · Cited by 392
Reifenberger, E., Boles, E., & Ciriacy, M. (1997) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1997.00324.x · Cited by 383
Self-citation
Cited by
Towards industrial pentose-fermenting yeast strains
Hahn-Hägerdal, B., Karhumaa, K., Fonseca, C., Spencer-Martins, I., & Gorwa-Grauslund, M. F. (2007) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-006-0827-2 · Cited by 783
Self-citationMetabolic engineering for improved fermentation of pentoses by yeasts
Jeffries, T. W., & Jin, Y.-S. (2004) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-003-1450-0 · Cited by 499
KUYPER, M., HARHANGI, H., STAVE, A., WINKLER, A., JETTEN, M., DELAAT, W., DENRIDDER, J., OPDENCAMP, H., VANDIJKEN, J., & PRONK, J. (2003) · FEMS Yeast Research · doi:10.1016/s1567-1356(03)00141-7 · Cited by 366
Evolutionary engineering of mixed-sugar utilization by a xylose-fermenting strain
KUYPER, M., TOIRKENS, M., DIDERICH, J., WINKLER, A., VANDIJKEN, J., & PRONK, J. (2005) · FEMS Yeast Research · doi:10.1016/j.femsyr.2005.04.004 · Cited by 341
Demeke, M. M., Dietz, H., Li, Y., Foulquié-Moreno, M. R., Mutturi, S., Deprez, S., Den Abt, T., Bonini, B. M., Liden, G., Dumortier, F., et al. (2013) · Biotechnology for Biofuels · doi:10.1186/1754-6834-6-89 · Cited by 331
Self-citation
Metadata
- Authors
- Hamacher, T., Becker, J., Gárdonyi, M., Hahn-Hägerdal, B., & Boles, E.
- Venue
- Microbiology
- Publisher
- Microbiology Society
- Published
- 2002-09-01
- Volume
- 148
- Issue
- 9
- Pages
- 2783-2788
- ISSN
- 1350-0872 (ISSN-L)
- 1465-2080
- OpenAlex
- W2151785115
- Cited by
- 317
- References
- 28
- FWCI
- 2.05
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Characterization of the xylose-transporting properties of yeast hexose transporters and their influence on xylose utilization. https://app.cochat.ai/papers/10.1099/00221287-148-9-2783
- The paper
- Hamacher, T., Becker, J., Gárdonyi, M., Hahn-Hägerdal, B., & Boles, E. (2002). Characterization of the xylose-transporting properties of yeast hexose transporters and their influence on xylose utilization. Microbiology. https://doi.org/10.1099/00221287-148-9-2783
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
