The DOI matches its Crossref record; 17 of the 17 references it cites were found in a registry or an index.
Mechanisms regulating the transport of acetic acid in Saccharomyces cerevisiae.
Abstract
Cells of the yeast Saccharomyces cerevisiae IGC 4072 grown in medium with acetic acid produced a mediated transport system for acetic acid that behaved as an electroneutral proton symport for the anionic form of the acid. The system could transport propionate and formate but not lactate and pyruvate. Uptake of labelled lactic acid was negligible, no mediated transport system activity for this acid being found. The acetate transporter was also found in cells grown in lactic acid or ethanol media, suggesting that the carrier did not require the presence of an external inducer. When cells were grown in lactic acid medium, uptake of labelled acetic acid, at pH 5.0, was biphasic and consistent with the presence of two distinct transport modes for the acid. One of these components corresponded to the acetate/proton symport, and the higher affinity system corresponded to a more general monocarboxylate carrier that could also transport lactate, pyruvate and propionate. Both systems were subject to repression by glucose, fructose, sucrose, maltose or galactose. In glucose-repressed cells, the undissociated form of the acids appeared to be the only one that could cross the plasma membrane, a diffusion mechanism being involved in the acid uptake. Under these growth conditions and when the extracellular pH was lower than that of the cytosol, accumulation of the acid could also be observed, it being a function of the delta pH.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“In S. cerevisiae, ADY2 (also called ATO1) encodes a permease-mediating uptake of acetate–propionate–formate present in ethanol or acetate-grown cells (Casal et al., 1996; Paiva et al., 2004; Pacheco et al., 2012).”
From Candida glabrata susceptibility to antifungals and phagocytosis is modulated by acetate (2015) · cites this paper as Casal et al., 1996
Citations
Cites
Transport and Diffusion Across Cell Membranes
Stein, W. D., & Lieb, W. R. (1986) · Elsevier eBooks · doi:10.1016/b978-0-12-664660-3.x5001-7 · Cited by 1,029
[64] The measurement of membrane potential and ΔpH in cells, organelles, and vesicles
Rottenberg, H. (1979) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(79)55066-6 · Cited by 798
Yeasts: Characteristics and identification
Spencer, J. F. T. (1984) · Food Microbiology · doi:10.1016/0740-0020(84)90068-6 · Cited by 680
Data for Biochemical Research (third edition)
Wood, E. (1987) · Biochemical Education · doi:10.1016/0307-4412(87)90110-5 · Cited by 519
Wagner, J. (1983) · Journal of Chromatography A · doi:10.1016/s0021-9673(01)88353-9 · Cited by 297
Rose, A. H. (1968) · Springer eBooks · doi:10.1007/978-1-4899-6567-7_6 · Cited by 256
Transport-limited fermentation and growth of Saccharomyces cerevisiae and its competitive inhibition
van Uden, N. O. P. (1967) · Archives of Microbiology · doi:10.1007/bf00406676 · Cited by 200
Transport of lactate and other short-chain monocarboxylates in the yeast Saccharomyces cerevisiae
C�ssio, F., Leo, C. J., & van Uden, N. O. P. (1987) · Applied and Environmental Microbiology · doi:10.1128/aem.53.3.509-513.1987 · Cited by 178
Effect of yeast killer toxin on sensitive cells of Saccharomyces cerevisiae.
de la Peña, P., Barros, F., Gascón, S., Lazo, P. S., & Ramos, S. (1981) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)68636-9 · Cited by 133
Transport of lactate and other short-chain monocarboxylates in the yeast Candida utilis
Leão, C., & van Uden, N. O. P. (1986) · Applied Microbiology and Biotechnology · doi:10.1007/bf00257039 · Cited by 58
Transport of l(-)malic acid and other dicarboxylic acids in the yeast Candida sphaerica
Côrte‐Real, M., Leão, C., & van Uden, N. O. P. (1989) · Applied Microbiology and Biotechnology · doi:10.1007/bf00270793 · Cited by 48
Casal, M., & Leão, C. (1995) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/0167-4889(95)00067-3 · Cited by 38
Eddy, A. A., & Hopkins, P. (1985) · Biochemical Journal · doi:10.1042/bj2310291 · Cited by 38
Regulation of glucose transport in Saccharomyces cerevisiae
Fuhrmann, G. F., & Völker, B. (1992) · Journal of Biotechnology · doi:10.1016/0168-1656(92)90026-6 · Cited by 36
Transport of lactic acid in Kluyveromyces marxianus: Evidence for a monocarboxylate uniport
Fonseca, Á., Spencer‐Martins, I., & van Uden, N. O. P. (1991) · Yeast · doi:10.1002/yea.320070803 · Cited by 17
Cássio, F., Côrte‐Real, M., & Leão, C. (1993) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(93)90276-6 · Cited by 14
Lack of lactate-proton symport activity inpck1mutants ofSaccharomyces cerevisiae
Casai, M., Blázquez, M. A., Gamo, F., Gancedo, C., & Leão, C. (1995) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1995.tb07536.x · Cited by 14
Cited by
Sorted by citations
Fermentation of lignocellulosic hydrolysates. II: inhibitors and mechanisms of inhibition
Palmqvist, E. A., & Hahn‐Hägerdal, B. (2000) · Bioresource Technology · doi:10.1016/s0960-8524(99)00161-3 · Cited by 2,518
Fermentation of lignocellulosic hydrolysates. I: inhibition and detoxification
Palmqvist, E. A., & Hahn‐Hägerdal, B. (2000) · Bioresource Technology · doi:10.1016/s0960-8524(99)00160-1 · Cited by 1,369
The proton-linked monocarboxylate transporter (MCT) family: structure, function and regulation
Halestrap, A. P., & Price, N. T. (1999) · Biochemical Journal · doi:10.1042/bj3430281 · Cited by 1,346
van Maris, A. J. A., Abbott, D. A., Bellissimi, E., van den Brink, J., Kuyper, M., Luttik, M. A. H., Wisselink, H. W., Scheffers, W. A., van Dijken, J. P., & Pronk, J. T. (2006) · Antonie van Leeuwenhoek · doi:10.1007/s10482-006-9085-7 · Cited by 546
Transcriptional control of nonfermentative metabolism in the yeast Saccharomyces cerevisiae
Schüller, H. (2003) · Current Genetics · doi:10.1007/s00294-003-0381-8 · Cited by 450
Metadata
- Authors
- Casal, M., Cardoso, H., & Leao, C.
- Affiliations
- University of Minho (PT)
- Department of Biology, University of Minho, 4719 Braga Codex, Portugal
- ORCID
- Casal, Margarida
- Venue
- Microbiology
- Publisher
- Microbiology Society
- Published
- 1996-06-01
- Volume
- 142
- Issue
- 6
- Pages
- 1385-1390
- ISSN
- 1350-0872 (ISSN-L)
- 1465-2080
- OpenAlex
- W2115422703
- Cited by
- 200
- References
- 17
- Citations by year
- 2026: 4 · 2025: 8 · 2024: 5 · 2023: 6 · 2022: 10 · 2021: 14 · 2020: 9 · 2019: 6 · 2018: 9 · 2017: 9 · 2016: 8 · 2015: 11 · 2014: 6 · 2013: 8 · 2012: 10
- FWCI
- 1.40
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Johns Hopkins University
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Health Sciences › Medicine › Oncology › Drug Transport and Resistance Mechanisms
- Keywords
- Saccharomyces cerevisiae, proton symport, glucose repression, lactate utilization, pyruvate uptake, pH gradient, acid accumulation
- MeSH terms
- Acetates, Biological Transport, Carbohydrates, Carrier Proteins, Diffusion, Formates, Fungal Proteins, Lactates, Propionates, Protons, Pyruvates, Saccharomyces cerevisiae, Pyruvic Acid, Acetic Acid, Lactic Acid, Monocarboxylic Acid Transporters
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
