Clostridium lavalense sp. nov., a glycopeptide-resistant species isolated from human faeces
Abstract
Two vancomycin-resistant, strictly anaerobic, Gram-positive, rod-shaped, spore-forming organisms (strains CCRI-9842(T) and CCRI-9929) isolated from human faecal specimens in Québec, Canada, and Australia were characterized using phenotypic, biochemical and molecular taxonomic methods. Pairwise analysis of the 16S rRNA gene sequences showed that both strains were closely related to each other genetically (displaying 99.2 % sequence similarity) and represented a previously unknown subline within the Clostridium coccoides rRNA group of organisms (rRNA cluster XIVa of the genus Clostridium). Strains CCRI-9842(T) and CCRI-9929 used carbohydrates as fermentable substrates, producing acetic acid as the major product of glucose metabolism. The novel strains were most closely related to Clostridium asparagiforme, Clostridium bolteae and Clostridium clostridioforme, but morphological, biochemical and phylogenetic studies demonstrated that they represent a previously unidentified species of the genus Clostridium. This was confirmed by the unique cellular fatty acid composition of strains CCRI-9842(T) and CCRI-9929. Therefore, on the basis of data from the polyphasic taxonomic analysis, it is proposed that strains CCRI-9842(T) and CCRI-9929 represent a novel species of the genus Clostridium, for which the name Clostridium lavalense sp. nov. is proposed. The type strain is CCRI-9842(T) (=CCUG 54291(T)=JCM 14986(T)=NML 03-A-015(T)).
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A reduction in the number of Ruminococcus and Clostridium could potentially impact the synthesis of antimicrobial peptides, leading to colonization of detrimental microorganisms in the intestinal barrier and contributing to the progression of liver disease.
“Previous studies have confirmed that a reduction in the number of Ruminococcus and Clostridium could potentially impact the synthesis of antimicrobial peptides. As a result, this can lead to the colonization of detrimental microorganisms in the intestinal barrier, consequently contributing to the progression of liver disease (Domingo et al., 2009).”
From Lactiplantibacillus plantarum N4 ameliorates lipid metabolism and gut microbiota structure in high fat diet-fed rats (2024) · cites this paper as Domingo et al., 2009
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- Authors
- Domingo, M.-C., Huletsky, A., Boissinot, M., Helie, M.-C., Bernal, A., Bernard, K. A., Grayson, M. L., Picard, F. J., & Bergeron, M. G.
- Affiliations
- Centre hospitalier universitaire de Québec (CA)
- Health Canada (CA)
- Austin Hospital (AU)
- Venue
- INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
- Publisher
- Microbiology Society
- Published
- 2009-03-01
- Volume
- 59
- Issue
- 3
- Pages
- 498-503
- ISSN
- 1466-5026 (ISSN-L)
- 1466-5034
- OpenAlex
- W2151830179
- Cited by
- 29
- References
- 20
- Citations by year
- 2026: 1 · 2024: 2 · 2023: 3 · 2022: 2 · 2020: 4 · 2019: 2 · 2018: 2 · 2017: 3 · 2016: 4 · 2015: 2 · 2013: 3 · 2012: 1
- FWCI
- 0.69
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Clostridium difficile and Clostridium perfringens research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Bacterial Identification and Susceptibility Testing
- Keywords
- novel bacterial species
- MeSH terms
- Anti-Bacterial Agents, Clostridium, DNA, Bacterial, Fatty Acids, Feces, Glycopeptides, Humans, Microbial Sensitivity Tests, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S, Species Specificity, Bacterial Typing Techniques, Sequence Analysis, DNA, Drug Resistance, Bacterial
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