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Cited by 59Probiotics and Fermented FoodsReproductive tract infections researchGut microbiota and health

Vaginal colonization and activity of the probiotic bacterium Lactobacillus fermentum L23 in a murine model of vaginal tract infection

Pascual, Liliana, Ruiz, Francisco, Giordano, Walter, Barberis, Isabel Lucila·2010·Journal of Medical Microbiology
DOI 10.1099/jmm.0.012583-0
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Abstract

A strain of Lactobacillus, identified as Lactobacillus fermentum L23, was selected from among 100 strains isolated from vaginal swabs of healthy, non-pregnant, pre-menopausal women. L. fermentum L23 was chosen on the basis of its bacteriocinogenic ability and its properties relevant to colonization, i.e. self-aggregation, adherence to vaginal epithelial cells and co-aggregation with bacterial pathogens. The antimicrobial preventative and curative effects produced by the probiotic L. fermentum L23 administered locally against Escherichia coli in a murine vaginal tract infection model were studied. One dose of the human strain L23 containing 10 8 c.f.u. ml−1 colonized and persisted in the vaginal tract of the female BALB/c mice for 5 days. Infection with the pathogen at 10 6 c.f.u. ml−1 in the vaginal tract was maintained for more than 7 days. A single dose of L23 administered 24 h pre-infection inhibited E. coli growth on day 3 post-infection, showing the preventative effect displayed by this Lactobacillus strain. Treatment with L. fermentum L23 during the post-infection period showed complete inhibition of pathogen growth from day 5. Thus, this in vivo study indicated that the probiotic bacterium L. fermentum L23 produced both preventative and curative effects on E. coli growth. The beneficial properties and the production of antimicrobial metabolites may act in situ to inhibit a pathogenic micro-organism within the vaginal environment. Strain L23 could be a good natural alternative to other therapies used for genital infections.

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  • Lm. fermentum is not typically a dominant species in the gastrointestinal tract or vagina, but its established probiotic characteristics make it a promising candidate.

    “WhileLm. fermentumis not typically a dominant species in the gastrointestinal tract or in the vagina, its established probiotic characteristics make it a promising candidate [48, 50–52].”

    From Women's health is a team effort: probiogenomics supports the development of a multi-species vaginal probiotic (2026) · cites this paper as [52]

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Authors
Pascual, L., Ruiz, F., Giordano, W., & Barberis, I. L.
Affiliations
  • Universidad Nacional de Río Cuarto (AR)
  • Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto (UNRC), 5800 Río Cuarto, Córdoba, Argentina
ORCID
Giordano, Walter
Venue
Journal of Medical Microbiology
Publisher
Microbiology Society
Published
2010-03-01
Volume
59
Issue
3
Pages
360-364
ISSN
  • 0022-2615 (ISSN-L)
  • 1473-5644
DOI
10.1099/jmm.0.012583-0
OpenAlex
W2098749103
Cited by
59
References
37
Citations by year
2026: 2 · 2024: 2 · 2023: 3 · 2022: 4 · 2021: 6 · 2020: 2 · 2019: 4 · 2018: 4 · 2017: 3 · 2016: 4 · 2015: 4 · 2014: 7 · 2013: 4 · 2012: 5
FWCI
5.29
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Medical Microbiology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Consejo Nacional de Investigaciones Científicas y Técnicas
  • Secretaría de Ciencia y Técnica, Universidad Nacional de Río Cuarto
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
Keywords
Lactobacillus fermentum, probiotics, vaginal colonization, Escherichia coli, bacteriocins, antimicrobial activity, self-aggregation, vaginal microbiota, genital tract infection
MeSH terms
Animals, Antibiosis, Anti-Bacterial Agents, Bacteriocins, Disease Models, Animal, DNA, Bacterial, Escherichia coli Infections, Female, Humans, Mice, Inbred BALB C, Molecular Sequence Data, Vagina, Vaginitis, Colony Count, Microbial, Sequence Analysis, DNA, Probiotics, Mice, Limosilactobacillus fermentum

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