The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Alginate production affects Pseudomonas aeruginosa biofilm development and architecture, but is not essential for biofilm formation
Abstract
Extracellular polymers can facilitate the non-specific attachment of bacteria to surfaces and hold together developing biofilms. This study was undertaken to qualitatively and quantitatively compare the architecture of biofilms produced by Pseudomonas aeruginosa strain PAO1 and its alginate-overproducing (mucA22) and alginate-defective (algD) variants in order to discern the role of alginate in biofilm formation. These strains, PAO1, Alg+PAO mucA22 and Alg−PAO algD, tagged with green fluorescent protein, were grown in a continuous flow cell system to characterize the developmental cycles of their biofilm formation using confocal laser scanning microscopy. Biofilm Image Processing (bip) and Community Statistics (comstat) software programs were used to provide quantitative measurements of the two-dimensional biofilm images. All three strains formed distinguishable biofilm architectures, indicating that the production of alginate is not critical for biofilm formation. Observation over a period of 5 days indicated a three-stage development pattern consisting of initiation, establishment and maturation. Furthermore, this study showed that phenotypically distinguishable biofilms can be quantitatively differentiated.
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Cites
The Involvement of Cell-to-Cell Signals in the Development of a Bacterial Biofilm
Davies, D. G., Parsek, M. R., Pearson, J. P., Iglewski, B. H., Costerton, J. W., & Greenberg, E. P. (1998) · Science · doi:10.1126/science.280.5361.295 · Cited by 3,396
Pseudomonas aeruginosa Displays Multiple Phenotypes during Development as a Biofilm
Sauer, K., Camper, A. K., Ehrlich, G. D., Costerton, J. W., & Davies, D. G. (2002) · Journal of Bacteriology · doi:10.1128/jb.184.4.1140-1154.2002 · Cited by 1,691 · cited 2×
“Similarly, other studies have shown that motility is not a prerequisite for successful biofilm development (Heydorn et al., 2002; Sauer et al., 2002).”
Cited by
The role of bacterial biofilms in chronic infections
Bjarnsholt, T. (2013) · APMIS · doi:10.1111/apm.12099 · Cited by 1,080
Bjarnsholt, T., Alhede, M., Alhede, M., Eickhardt-Sørensen, S. R., Moser, C., Kühl, M., Jensen, P. Ø., & Høiby, N. (2013) · Trends in Microbiology · doi:10.1016/j.tim.2013.06.002 · Cited by 764
Self-citationAction of Antimicrobial Peptides against Bacterial Biofilms
Yasir, M., Willcox, M. D. P., & Dutta, D. (2018) · Materials · doi:10.3390/ma11122468 · Cited by 319
Baker, P., Hill, P. J., Snarr, B. D., Alnabelseya, N., Pestrak, M. J., Lee, M. J., Jennings, L. K., Tam, J., Melnyk, R. A., Parsek, M. R., et al. (2016) · Science Advances · doi:10.1126/sciadv.1501632 · Cited by 284
Antibiofilm Activity of an Exopolysaccharide from Marine Bacterium Vibrio sp. QY101
Jiang, P., Li, J., Han, F., Duan, G., Lu, X., Gu, Y., & Yu, W. (2011) · PLoS ONE · doi:10.1371/journal.pone.0018514 · Cited by 165
Metadata
- Authors
- Stapper, A. P., Narasimhan, G., Ohman, D. E., Barakat, J., Hentzer, M., Molin, S., Kharazmi, A., Høiby, N., & Mathee, K.
- Venue
- Journal of Medical Microbiology
- Publisher
- Microbiology Society
- Published
- 2004-07-01
- Volume
- 53
- Issue
- 7
- Pages
- 679-690
- ISSN
- 0022-2615 (ISSN-L)
- 1473-5644
- OpenAlex
- W2134256289
- Cited by
- 186
- References
- 2
- FWCI
- 2.73
- Type
- journal article
- Language
- en
- Open access
- closed
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- Stapper, A. P., Narasimhan, G., Ohman, D. E., Barakat, J., Hentzer, M., Molin, S., Kharazmi, A., Høiby, N., & Mathee, K. (2004). Alginate production affects Pseudomonas aeruginosa biofilm development and architecture, but is not essential for biofilm formation. Journal of Medical Microbiology. https://doi.org/10.1099/jmm.0.45539-0
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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