Antibiofilm Activity of an Exopolysaccharide from Marine Bacterium Vibrio sp. QY101
Open accessAbstract
Bacterial exopolysaccharides have always been suggested to play crucial roles in the bacterial initial adhesion and the development of complex architecture in the later stages of bacterial biofilm formation. However, Escherichia coli group II capsular polysaccharide was characterized to exert broad-spectrum biofilm inhibition activity. In this study, we firstly reported that a bacterial exopolysaccharide (A101) not only inhibits biofilm formation of many bacteria but also disrupts established biofilm of some strains. A101 with an average molecular weight of up to 546 KDa, was isolated and purified from the culture supernatant of the marine bacterium Vibrio sp. QY101 by ethanol precipitation, iron-exchange chromatography and gel filtration chromatography. High performance liquid chromatography traces of the hydrolyzed polysaccharides showed that A101 is primarily consisted of galacturonic acid, glucuronic acid, rhamnose and glucosamine. A101 was demonstrated to inhibit biofilm formation by a wide range of Gram-negative and Gram-positive bacteria without antibacterial activity. Furthermore, A101 displayed a significant disruption on the established biofilm produced by Pseudomonas aeruginosa, but not by Staphylococcus aureus. Importantly, A101 increased the aminoglycosides antibiotics' capability of killing P. aeruginosa biofilm. Cell primary attachment to surfaces and intercellular aggregates assays suggested that A101 inhibited cell aggregates of both P. aeruginosa and S. aureus, while the cell-surface interactions inhibition only occurred in S. aureus, and the pre-formed cell aggregates dispersion induced by A101 only occurred in P. aeruginosa. Taken together, these data identify the antibiofilm activity of A101, which may make it potential in the design of new therapeutic strategies for bacterial biofilm-associated infections and limiting biofilm formation on medical indwelling devices. The found of A101 antibiofilm activity may also promote a new recognition about the functions of bacterial exopolysaccharides.
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It has been suggested that EPS can inhibit initial auto-aggregation of biofilm-forming bacteria by either interrupting communications among cells or by weakening cell membranes, and this ability is considered dose-dependent.
“It has been suggested that EPS can inhibit the initial auto-aggregation of biofilm-forming bacteria by either interrupting communications among cells or by weakening cell membranes [152], and this ability is considered to be dose-dependent [153].”
From Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods (2021) · cites this paper as [153]
The Results section, “A101 inhibits cell-surface adherence and intercellular adhesion” (page unavailable), reports that A101 reduced cell aggregates in two bacterial strains. The Discussion proposes interference with cell–cell adhesion, not interruption of cellular communication or weakening of cell membranes. The dose-dependent clause is attached to a different citation, [153], so it is not judged here. (LLM, conf=0.88)
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- Authors
- Jiang, P., Li, J., Han, F., Duan, G., Lu, X., Gu, Y., & Yu, W.
- Affiliations
- Ocean University of China (CN)
- Northwestern Polytechnical University (CN)
- Corresponding author
- Yu, Wengong
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2011-04-07
- Volume
- 6
- Issue
- 4
- Pages
- e18514
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC3072402
- OpenAlex
- W2067983977
- Cited by
- 165
- References
- 83
- Citations by year
- 2026: 6 · 2025: 5 · 2024: 11 · 2023: 10 · 2022: 17 · 2021: 12 · 2020: 21 · 2019: 16 · 2018: 8 · 2017: 12 · 2016: 19 · 2015: 4 · 2014: 11 · 2013: 7 · 2012: 5
- FWCI
- 3.11
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- PLoS ONE (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Figshare (submittedVersion, cc-by)
- Funders
- Ministry of Education, India
- Program for Changjiang Scholars and Innovative Research Team in University (IRT0944)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Physical Sciences › Engineering › Ocean Engineering › Marine Biology and Environmental Chemistry
- Keywords
- antibiofilm activity, bacterial exopolysaccharides, marine bacteria, Pseudomonas aeruginosa biofilm, Staphylococcus aureus biofilm, biofilm eradication, cell aggregation, cell surface binding, aminoglycoside synergy, polysaccharide structure, biofilm-associated infections
- MeSH terms
- Anti-Bacterial Agents, Chromatography, High Pressure Liquid, Polysaccharides, Bacterial, Pseudomonas aeruginosa, Staphylococcus aureus, Vibrio, Spectroscopy, Fourier Transform Infrared, Biofilms
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Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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