The DOI matches its Crossref record; 47 of the 47 references it cites were found in a registry or an index.
Microcolony formation: a novel biofilm model of Pseudomonas aeruginosa for the cystic fibrosis lung
Abstract
Pseudomonas aeruginosa colonizing the lung of cystic fibrosis patients is responsible for a decline in health and poor prognosis for these patients. Once established, growth of P. aeruginosa in microcolonies makes it very difficult to eradicate the organisms by antimicrobial treatment. An artificial sputum medium was developed to mimic growth of P. aeruginosa in the cystic fibrosis lung habitat and it was found that the organisms grew in tight microcolonies attached to sputum components. Several genes, such as algD, oprF and lasR but not fliC, were required for tight microcolony formation. Among the sputum components, amino acids, lecithin, DNA, salt and low iron were required for tight microcolony formation. Amino acids were also shown to be responsible for various other cystic-fibrosis-specific phenotypes of P. aeruginosa, such as diversification of colony morphology, alterations in LPS structure and hyperexpression of OprF. Since the amino acid content of sputum is elevated in severe lung disease, it is suggested that the tight microcolony biofilm is maintained in these conditions and that they contribute to the vicious cycle of disease severity and failure to eradicate the organism. Thus, growth of P. aeruginosa in artificial sputum medium is an appropriate model of chronic lung colonization and may be useful for evaluating therapeutic procedures and studying antibiotic-resistance mechanisms.
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Cited by
Pseudomonas aeruginosa : new insights into pathogenesis and host defenses
Gellatly, S. L., & Hancock, R. E. W. (2013) · Pathogens and Disease · doi:10.1111/2049-632x.12033 · Cited by 1,295
Signals, Regulatory Networks, and Materials That Build and Break Bacterial Biofilms
Karatan, E., & Watnick, P. (2009) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00041-08 · Cited by 1,048
Rumbaugh, K. P., & Sauer, K. (2020) · Nature Reviews Microbiology · doi:10.1038/s41579-020-0385-0 · Cited by 907
Brackman, G., Cos, P., Maes, L., Nelis, H. J., & Coenye, T. (2011) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.00045-11 · Cited by 561
Airway Surface Dehydration in Cystic Fibrosis: Pathogenesis and Therapy
Boucher, R. C. (2007) · Annual Review of Medicine · doi:10.1146/annurev.med.58.071905.105316 · Cited by 512
Metadata
- Authors
- Sriramulu, D. D., Lünsdorf, H., Lam, J. S., & Römling, U.
- Venue
- Journal of Medical Microbiology
- Publisher
- Microbiology Society
- Published
- 2005-07-01
- Volume
- 54
- Issue
- 7
- Pages
- 667-676
- ISSN
- 0022-2615 (ISSN-L)
- 1473-5644
- OpenAlex
- W2132211475
- Cited by
- 366
- References
- 47
- FWCI
- 2.42
- Type
- journal article
- Language
- en
- Open access
- closed
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- Sriramulu, D. D., Lünsdorf, H., Lam, J. S., & Römling, U. (2005). Microcolony formation: a novel biofilm model of Pseudomonas aeruginosa for the cystic fibrosis lung. Journal of Medical Microbiology. https://doi.org/10.1099/jmm.0.45969-0
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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