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Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels
Open accessAbstract
The morphological heterogeneity of lipopolysaccharides (LPSs) among salmonella mutants with different LPS chemotypes was analyzed in silver-stained polyacrylamide gels. The biochemical differences in the LPS chemotypes were reflected in the unique profiles of the purified LPSs. The LPS profiles in the whole-cell lysates were also unique for each chemotype. (Whole-cell lysates were assessed by a method which preferentially silver stains LPS and by a proteinase K digest of whole-cell lysates. The silver-stained LPS profiles of proteinase K-digested lysates were similar to the homologous purified LPS and could be used to preliminarily characterize the LPS chemotype before purification.) In summary, biochemical variation in LPS composition can be detected in silver-stained polyacrylamide gels.
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Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
Cited by
Byrd, M. S., Sadovskaya, I., Vinogradov, E., Lu, H., Sprinkle, A. B., Richardson, S. H., Ma, L., Ralston, B., Parsek, M. R., Anderson, E. M., et al. (2009) · Molecular Microbiology · doi:10.1111/j.1365-2958.2009.06795.x · Cited by 386
A Functional Genomics Approach to Understand Variation in Cytokine Production in Humans
Li, Y., Oosting, M., Smeekens, S. P., Jaeger, M., Aguirre-Gamboa, R., Le, K. T. T., Deelen, P., Ricaño-Ponce, I., Schoffelen, T., Jansen, A. F. M., et al. (2016) · Cell · doi:10.1016/j.cell.2016.10.017 · Cited by 324
A Journey from Structure to Function of Bacterial Lipopolysaccharides
Di Lorenzo, F., Duda, K. A., Lanzetta, R., Silipo, A., De Castro, C., & Molinaro, A. (2022) · Chemical Reviews · doi:10.1021/acs.chemrev.0c01321 · Cited by 322
Participation of a cyanobacterial S layer in fine-grain mineral formation
Schultze-Lam, S., Harauz, G., & Beveridge, T. J. (1992) · Journal of Bacteriology · doi:10.1128/jb.174.24.7971-7981.1992 · Cited by 275
Polyethyleneimine is an effective permeabilizer of Gram negative bacteria
Helander, I. M., Alakomi, H.-L., Latva-Kala, K., & Koski, P. (1997) · Microbiology · doi:10.1099/00221287-143-10-3193 · Cited by 237
Metadata
- Authors
- Hitchcock, P. J., & Brown, T. M.
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 1983-04
- Volume
- 154
- Issue
- 1
- Pages
- 269-277
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- OpenAlex
- W1922098314
- Cited by
- 1,860
- References
- 1
- FWCI
- 105.26
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Hitchcock, P. J., & Brown, T. M. (1983). Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. Journal of Bacteriology. https://doi.org/10.1128/jb.154.1.269-277.1983
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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