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Cited by 262Antifungal resistance and susceptibilityFungal and yeast genetics researchBacterial biofilms and quorum sensing

The DOI matches its Crossref record; 39 of the 39 references it cites were found in a registry or an index.

Development of a simple model for studying the effects of antifungal agents on multicellular communities of Aspergillus fumigatus

Mowat, Eilidh, Butcher, John, Lang, Sue, Williams, Craig, Ramage, Gordon·2007·Journal of Medical Microbiology
DOI 10.1099/jmm.0.47247-0
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Abstract

Aspergillus fumigatus is an increasingly prevalent opportunistic fungal pathogen of various immunocompromised individuals. It has the ability to form filaments within the lungs, producing dense intertwined mycelial balls, which are difficult to treat. The aim of this study was to develop a suitable model of A. fumigatus to examine the effects of antifungal challenge on these intertwined filamentous communities. A. fumigatus NCPF 7367 growth conditions were optimized on both Thermanox coverslips and on flat-bottomed microtitre plates to establish optimal conidial seeding densities. Isolates were treated with itraconazole, voriconazole, amphotericin B and caspofungin and their overall killing efficiency was measured using an XTT formazan metabolic dye assay. This was compared with the CLSI (formerly NCCLS) methodology of broth microdilution of moulds (standard M38-A). It was shown that 1×10 5 conidia ml−1 in RPMI 1640 was the optimum concentration of spores for biofilm formation. Filamentous growth characteristics were not observed until 10 h incubation, followed by an exponential increase in the biofilm biomass (hyphae and extracellular material) and cellular activity (metabolism). When susceptibility testing of biofilms was compared with that of planktonic cells by CLSI broth microdilution testing, all antifungal drugs were at least 1000 times less effective at reducing the overall metabolic activity of 90 % of the cells. Overall, this study showed that A. fumigatus has the ability to form coherent multicellular biofilm structures that are resistant to the effects of antifungal drugs.

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Crossref · 10.1099/jmm.0.47247-0

Cites

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Citations

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Research works printed but not linked: 1

  1. 2002, Reference Method for Broth Microdilution Antifungal Susceptibility Testing of Filamentous Fungi . NCCLS document M38-A

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Metadata

Authors
Mowat, E., Butcher, J., Lang, S., Williams, C., & Ramage, G.
Affiliations
  • Glasgow Caledonian University (GB)
  • Microbiology Department, Yorkhill Hospital, Glasgow, UK
  • University of Glasgow (GB)
ORCID
Venue
Journal of Medical Microbiology
Publisher
Microbiology Society
Published
2007-09-01
Volume
56
Issue
9
Pages
1205-1212
ISSN
  • 0022-2615 (ISSN-L)
  • 1473-5644
DOI
10.1099/jmm.0.47247-0
OpenAlex
W2112677154
Cited by
259
References
39
Citations by year
2026: 7 · 2025: 11 · 2024: 10 · 2023: 12 · 2022: 18 · 2021: 18 · 2020: 26 · 2019: 17 · 2018: 13 · 2017: 10 · 2016: 22 · 2015: 20 · 2014: 9 · 2013: 13 · 2012: 18
FWCI
4.72
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Medical Microbiology (publishedVersion)
  • PubMed (publishedVersion)
  • ResearchOnline (acceptedVersion)
  • ENLIGHTEN (Jurnal Bimbingan dan Konseling Islam) (submittedVersion)
  • ResearchOnline (Glasgow Caledonian University) (submittedVersion)
Funders
  • Pfizer
  • Hospital for Sick Children
Topics
  • Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
Keywords
Aspergillus fumigatus, antifungal susceptibility, biofilm formation, filamentous growth, itraconazole, voriconazole, amphotericin B, caspofungin, antifungal resistance, planktonic versus biofilm cells, opportunistic fungal infection
MeSH terms
Antifungal Agents, Aspergillus fumigatus, Formazans, Microbial Sensitivity Tests, Biofilms, Hyphae, Microbial Viability

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