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Cited by 65Bacteriophages and microbial interactionsBacterial Genetics and BiotechnologyEvolution and Genetic Dynamics

The genetic basis of phage susceptibility, cross-resistance and host-range in Salmonella

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Adler, Benjamin A., Kazakov, Alexey E., Zhong, Crystal, Liu, Hualan, Kutter, Elizabeth, Lui, Lauren M., Nielsen, Torben N., Carion, Heloise +3 more·2021·Microbiology
DOI 10.1099/mic.0.001126PMC PMC8744999

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Abstract

Though bacteriophages (phages) are known to play a crucial role in bacterial fitness and virulence, our knowledge about the genetic basis of their interaction, cross-resistance and host-range is sparse. Here, we employed genome-wide screens in Salmonella enterica serovar Typhimurium to discover host determinants involved in resistance to eleven diverse lytic phages including four new phages isolated from a therapeutic phage cocktail. We uncovered 301 diverse host factors essential in phage infection, many of which are shared between multiple phages demonstrating potential cross-resistance mechanisms. We validate many of these novel findings and uncover the intricate interplay between RpoS, the virulence-associated general stress response sigma factor and RpoN, the nitrogen starvation sigma factor in phage cross-resistance. Finally, the infectivity pattern of eleven phages across a panel of 23 genome sequenced Salmonella strains indicates that additional constraints and interactions beyond the host factors uncovered here define the phage host range.

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Cited by

Sorted by citations

Authors
Adler, B. A., Kazakov, A. E., Zhong, C., Liu, H., Kutter, E., Lui, L. M., Nielsen, T. N., Carion, H., Deutschbauer, A. M., Mutalik, V. K., & Arkin, A. P.
Affiliations
  • University of California, San Francisco (US)
  • Lawrence Berkeley National Laboratory (US)
  • University of California, Berkeley (US)
  • The Evergreen State College (US)
  • Department of Bioengineering, University of California, Berkeley, Berkeley, California, USA
Corresponding author
  • Mutalik, Vivek K.
  • Arkin, Adam P.
ORCID
Venue
Microbiology
Publisher
Microbiology Society
Published
2021-12-01
Volume
167
Issue
12
ISSN
  • 1350-0872 (ISSN-L)
  • 1465-2080
DOI
10.1099/mic.0.001126
PMC
PMC8744999
OpenAlex
W4225823295
Cited by
65
References
1
Citations by year
2026: 10 · 2025: 16 · 2024: 14 · 2023: 10 · 2022: 6 · 2021: 6 · 2020: 3
FWCI
4.86
Type
journal article
Language
en
Open access
Licence
Locations
  • Microbiology (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nd)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • eScholarship (California Digital Library) (submittedVersion)
  • OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) (submittedVersion)
Funders
  • U.S. Department of Energy (-AC02-05CH11231, 05CH11231, DE AC02 05CH11231, DE-AC02-)
  • Innovative Genomics Institute
  • National Institutes of Health (1s10od018174-01, DE-AC02–05CH11231)
  • Office of Science (DEAC02–05CH11231, AC02-05CH11231, DE-AC02, 05CH11231)
  • National Center for Research Resources (S10RR029668, S10RR027303)
  • Biological and Environmental Research (05CH11231, DE-AC02 − 05CH11231, AC02-05CH11231)
  • Lawrence Berkeley National Laboratory (AC02‐05CH11231, DE-AC02-05CH11231, 05CH11231)
Topics
  • Physical Sciences › Environmental Science › Ecology › Bacteriophages and microbial interactions
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Evolution and Genetic Dynamics
Keywords
bacteriophage susceptibility, phage host range, Salmonella typhimurium, phage-host interactions, host factors, RpoS, RpoN, sigma factor, general stress response, nitrogen starvation, genome-wide screen
MeSH terms
Bacteriophages, Salmonella Phages, Salmonella typhimurium, Virulence, Host Specificity

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