The enigmatic lack of glucose utilization in Streptomyces clavuligerus is due to inefficient expression of the glucose permease gene
Abstract
Streptomyces clavuligerus ATCC 27064 is unable to use glucose but has genes for a glucose permease (glcP) and a glucose kinase (glkA). Transformation of S. clavuligerus 27064 with the Streptomyces coelicolor glcP1 gene with its own promoter results in a strain able to grow on glucose. The glcP gene of S. clavuligerus encodes a 475 amino acid glucose permease with 12 transmembrane segments. GlcP is a functional protein when expressed from the S. coelicolor glcP1 promoter and complements two different glucose transport-negative Escherichia coli mutants. Transcription studies indicate that the glcP promoter is very weak and does not allow growth on glucose. These results suggest that S. clavuligerus initially contained a functional glucose permease gene, like most other Streptomyces species, and lost the expression of this gene by adaptation to glucose-poor habitats.
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Various compounds have been studied as C3 precursor candidates to ensure proper formulation of fermentation media and improve clavulanic acid yield.
“As S. clavuligerus is unable to assimilate glucose, various compounds have been studied as C3 precursor candidates to ensure proper formulation of fermentation media and improve the yield of clavulanic acid (Aharonowitz and Demain, 1978; Garcia-Dominguez et al., 1989; Pérez-Redondo et al., 2010).”
From Fermentation Conditions that Affect Clavulanic Acid Production in Streptomyces clavuligerus: A Systematic Review (2016) · cites this paper as Pérez-Redondo et al., 2010
S. clavuligerus is unable to assimilate glucose.
“As S. clavuligerus is unable to assimilate glucose, various compounds have been studied as C3 precursor candidates to ensure proper formulation of fermentation media and improve the yield of clavulanic acid (Aharonowitz and Demain, 1978; Garcia-Dominguez et al., 1989; Pérez-Redondo et al., 2010).”
From Fermentation Conditions that Affect Clavulanic Acid Production in Streptomyces clavuligerus: A Systematic Review (2016) · cites this paper as Pérez-Redondo et al., 2010
The wild type strain of S. clavuligerus is unable to metabolize glucose and lacks expression of the glucose permease gene.
“The wild type strain of S. clavuligerus is unable to metabolize glucose, and further molecular studies revealed that the strain lacks the expression of the glucose permease gene (Garcia-Dominguez et al., 1989; Pérez-Redondo et al., 2010).”
From Fermentation Conditions that Affect Clavulanic Acid Production in Streptomyces clavuligerus: A Systematic Review (2016) · cites this paper as Pérez-Redondo et al., 2010
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Ser, H.-L., Law, J. W.-F., Chaiyakunapruk, N., Jacob, S. A., Palanisamy, U. D., Chan, K.-G., Goh, B.-H., & Lee, L.-H. (2016) · Frontiers in Microbiology · doi:10.3389/fmicb.2016.00522 · Cited by 90 · see citing works
- Authors
- Pérez-Redondo, R., Santamarta, I., Bovenberg, R., Martín, J. F., & Liras, P.
- Affiliations
- Instituto de Biotecnología, INBIOTEC, Parque Científico de León, Avda. Real °1, 24006 León, Spain
- Instituto de Biotecnología de León (ES)
- DSM Anti-Infectives B.V., Delft, The Netherlands
- ORCID
- Liras, Paloma
- Venue
- Microbiology
- Publisher
- Microbiology Society
- Published
- 2010-05-01
- Volume
- 156
- Issue
- 5
- Pages
- 1527-1537
- ISSN
- 1350-0872 (ISSN-L)
- 1465-2080
- OpenAlex
- W2155809353
- Cited by
- 27
- References
- 0
- Citations by year
- 2025: 1 · 2024: 2 · 2023: 1 · 2022: 1 · 2021: 1 · 2020: 1 · 2019: 3 · 2018: 2 · 2017: 3 · 2016: 2 · 2015: 3 · 2014: 2
- FWCI
- 0.72
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Microbiology (publishedVersion)
- PubMed (publishedVersion)
- University of Groningen research database (University of Groningen / Centre for Information Technology) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Health Sciences › Medicine › Pharmacology › Microbial Natural Products and Biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Keywords
- Streptomyces clavuligerus, glucose permease, glucose transport, glucokinase, Streptomyces coelicolor, gene complementation, promoter strength, gene loss, glucose utilization, transmembrane proteins, Escherichia coli mutants
- MeSH terms
- Amino Acid Sequence, Bacterial Proteins, Base Sequence, DNA, Bacterial, Escherichia coli, Genes, Bacterial, Genetic Complementation Test, Glucokinase, Glucose, Molecular Sequence Data, Phosphoenolpyruvate Sugar Phosphotransferase System, Promoter Regions, Genetic, Recombination, Genetic, Streptomyces, Streptomyces coelicolor
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