The DOI matches its Crossref record.
Role of Vibrio polysaccharide (vps) genes in VPS production, biofilm formation and Vibrio cholerae pathogenesis
Open accessAbstract
Biofilm formation enhances the survival and persistence of the facultative human pathogen Vibrio cholerae in natural ecosystems and its transmission during seasonal cholera outbreaks. A major component of the V. cholerae biofilm matrix is the Vibrio polysaccharide (VPS), which is essential for development of three-dimensional biofilm structures. The vps genes are clustered in two regions, the vps-I cluster (vpsU,vpsA–K, VC0916–27) and the vps-II cluster (vpsL–Q, VC0934–39), separated by an intergenic region containing the rbm gene cluster that encodes biofilm matrix proteins. In-frame deletions of the vps clusters and genes encoding matrix proteins drastically altered biofilm formation phenotypes. To determine which genes within the vps gene clusters are required for biofilm formation and VPS synthesis, we generated in-frame deletion mutants for all the vps genes. Many of these mutants exhibited reduced capacity to produce VPS and biofilms. Infant mouse colonization assays revealed that mutants lacking either vps clusters or rbmA(encoding secreted matrix protein RbmA) exhibited a defect in intestinal colonization compared to the wild-type. Understanding the roles of the various vps gene products will aid in the biochemical characterization of the VPS biosynthetic pathway and elucidate how vps gene products contribute to VPS biosynthesis, biofilm formation and virulence in V. cholerae.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Hobley, L., Harkins, C., MacPhee, C. E., & Stanley-Wall, N. R. (2015) · FEMS Microbiology Reviews · doi:10.1093/femsre/fuv015 · Cited by 581
“Using a systematic mutagenesis approach, it was identified that the single mutant strains fell into six phenotypic classes with the majority of the 18 genes in the two clusters being involved or required for VPS production, and therefore biofilm formation (Fong et al. 2010).”
BackgroundMethodInfluentialMolecular Architecture and Assembly Principles of Vibrio cholerae Biofilms
Berk, V., Fong, J. C. N., Dempsey, G. T., Develioglu, O. N., Zhuang, X., Liphardt, J., Yildiz, F. H., & Chu, S. (2012) · Science · doi:10.1126/science.1222981 · Cited by 385
Self-citationCommensal and Pathogenic Escherichia coli Metabolism in the Gut
Conway, T., & Cohen, P. S. (2015) · Microbiology Spectrum · doi:10.1128/microbiolspec.mbp-0006-2014 · Cited by 303
Environmental reservoirs and mechanisms of persistence of Vibrio cholerae
Lutz, C., Erken, M., Noorian, P., Sun, S., & McDougald, D. (2013) · Frontiers in Microbiology · doi:10.3389/fmicb.2013.00375 · Cited by 286
Antibiofilm Activity of an Exopolysaccharide from Marine Bacterium Vibrio sp. QY101
Jiang, P., Li, J., Han, F., Duan, G., Lu, X., Gu, Y., & Yu, W. (2011) · PLoS ONE · doi:10.1371/journal.pone.0018514 · Cited by 165
Metadata
- Authors
- Fong, J. C. N., Syed, K. A., Klose, K. E., & Yildiz, F. H.
- Venue
- Microbiology
- Publisher
- Microbiology Society
- Published
- 2010-09-01
- Volume
- 156
- Issue
- 9
- Pages
- 2757-2769
- ISSN
- 1350-0872 (ISSN-L)
- 1465-2080
- OpenAlex
- W2165017841
- Cited by
- 245
- References
- 0
- FWCI
- 5.16
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Role of Vibrio polysaccharide (vps) genes in VPS production, biofilm formation and Vibrio cholerae pathogenesis. https://app.cochat.ai/papers/10.1099/mic.0.040196-0
- The paper
- Fong, J. C. N., Syed, K. A., Klose, K. E., & Yildiz, F. H. (2010). Role of Vibrio polysaccharide (vps) genes in VPS production, biofilm formation and Vibrio cholerae pathogenesis. Microbiology. https://doi.org/10.1099/mic.0.040196-0
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
