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Cited by 32Fungal and yeast genetics researchAntifungal resistance and susceptibilityFungal Infections and Studies

Candida albicans strains deficient in CHS7, a key regulator of chitin synthase III, exhibit morphogenetic alterations and attenuated virulence

Sanz, María, Carrano, Lucia, Jiménez, Cristina, Candiani, Gianpaolo, Trilla, José A., Durán, Angel, Roncero, César·2005·Microbiology
DOI 10.1099/mic.0.28093-0
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Abstract

Chitin is a structural polysaccharide present in most fungal cell walls, whose synthesis depends on a family of enzymic activities named chitin synthases (CSs). The specific role of each of them, as well as of their regulatory proteins, in cell morphogenesis and virulence is not well understood. Here, it is shown that most chitin synthesis in Candida albicans, one of the fungi most commonly isolated from opportunistic mycoses and infections, depends on CHS7. Thus,C. albicans chs7 Δ null mutants showed reduced levels of chitin and CS activity, and were resistant to Calcofluor. Despite the sequence similarity and functional relationship with ScChs7p, CaChs7p was unable to restore CSIII activity in a Saccharomyces cerevisiae chs7 Δ null mutant, because it was unable to direct ScChs3p export from the endoplasmic reticulum.C. albicans chs7 Δ null mutants did not show any defect in growth rate, but yeast cells displayed minor morphogenetic defects affecting septum formation, and showed an increased tendency to form filaments. CaChs7p was not required for germ-tube emission, and null mutant strains underwent the dimorphic transition correctly. However, colony morphology appeared distinctively affected.chs7 Δ hyphae were very curved and had irregular lateral walls, resulting in very compact colonies that seemed unable to spread out radially on the surface, unlike the wild-type. This growth pattern may be associated with the reduced virulence and high clearance rate observed when the chs7 Δ strain was used in a murine model of infection. Therefore, CaChs7p is required for normal hyphal morphogenesis, suggesting that in C. albicans CSIII plays an important role in maintaining cell wall integrity, being essential when invading surrounding tissues.

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  • Chs3p is responsible for the deposition of the majority of chitin located in the lateral wall and chitin ring but not in the chitin disk of the primary septum.

    “CW-staining, specific for chitin, was comparable to the wild type strain except for chs3Δ cells that were CW-positive only at the thin line of the septa, an observation in agreement with Chs3p being responsible for the deposition of the majority of chitin located in the lateral wall and chitin ring but not in the chitin disk of the primary septum [48, 49].”

    From Genomic and functional analyses unveil the response to hyphal wall stress in Candida albicans cells lacking β(1,3)-glucan remodeling (2016) · cites this paper as [48]

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Authors
Sanz, M., Carrano, L., Jiménez, C., Candiani, G., Trilla, J. A., Durán, A., & Roncero, C.
Affiliations
  • Consejo Superior de Investigaciones Científicas (ES)
  • Vicuron Pharmaceuticals, Gerenzano, Italy
  • Instituto de Microbiología Bioquímica y Departamento de Microbiología y Genética, CSIC/Universidad de Salamanca, Edificio Departamental, R-219, Avda Campo Charro s/n, 37007 Salamanca, Spain
ORCID
Venue
Microbiology
Publisher
Microbiology Society
Published
2005-08-01
Volume
151
Issue
8
Pages
2623-2636
ISSN
  • 1350-0872 (ISSN-L)
  • 1465-2080
DOI
10.1099/mic.0.28093-0
OpenAlex
W2021838894
Cited by
32
References
43
Citations by year
2026: 2 · 2025: 2 · 2024: 1 · 2022: 2 · 2019: 1 · 2018: 5 · 2016: 2 · 2015: 1 · 2014: 1 · 2013: 3 · 2012: 1
FWCI
0.62
Type
journal article
Language
en
Open access
closed
Locations
  • Microbiology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
  • Health Sciences › Medicine › Epidemiology › Fungal Infections and Studies
Keywords
Candida albicans, chitin synthesis, hyphal morphogenesis, fungal virulence, cell wall integrity, dimorphic transition, germ tube formation, septum formation, mouse infection model
MeSH terms
Animals, Candida albicans, Chitin Synthase, Membrane Proteins, Morphogenesis, Mutation, Virulence

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