In vitro and in vivo specificity of ubiquitination and degradation of STAT1 and STAT2 by the V proteins of the paramyxoviruses simian virus 5 and human parainfluenza virus type 2
Abstract
Previous work has documented that the V protein of simian virus 5 (SV5) targets STAT1 for proteasome-mediated degradation, whilst the V protein of human parainfluenza virus type 2 (hPIV2) targets STAT2. Here, it was shown that the processes of ubiquitination and degradation could be reconstructed in vitro by using programmed rabbit reticulocyte lysates. Using this system, the addition of bacterially expressed and purified SV5 V protein to programmed lysates was demonstrated to result in the polyubiquitination and degradation of in vitro-translated STAT1, but only if human STAT2 was also present. Surprisingly, in the same assay, purified hPIV2 V protein induced the polyubiquitination of both STAT1 and STAT2. In the light of these in vitro results, the specificity of degradation of STAT1 and STAT2 by SV5 and hPIV2 in tissue-culture cells was re-examined. As previously reported, STAT1 could not be detected in human cells that expressed SV5 V protein constitutively, whilst STAT2 could not be detected in human cells that expressed hPIV2 V protein, although the levels of STAT1 may also have been reduced in some human cells infected with hPIV2. In contrast, STAT1 could not be detected, whereas STAT2 remained present, in a variety of animal cells, including canine (MDCK) cells, that expressed the V protein of either SV5 or hPIV2. Thus, the V protein of SV5 appears to be highly specific for STAT1 degradation, but the V protein of hPIV2 is more promiscuous.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The V protein of simian virus 5 binds the CRL4 adaptor DDB1, bringing STAT2/STAT1 heterodimers into proximity, and degradation of STAT1 diminishes interferon response.
“The V protein of simian virus 5 (SV5 V) binds the CRL4 adaptor DDB1, bringing in proximity the host STAT2/STAT1 heterodimers.Ubiquitination and further degradation of STAT1 largely diminishes interferon response to viral infection.99,100”
From Chasing molecular glue degraders: screening approaches (2022) · cites this paper as [100]
Cites
The Ubiquitin-Proteasome Proteolytic Pathway: Destruction for the Sake of Construction
Glickman, M. H., & Ciechanover, A. J. (2002) · Physiological Reviews · doi:10.1152/physrev.00027.2001 · Cited by 4,216 · see citing works
Themes and variations on ubiquitylation
Weissman, A. M. (2001) · Nature Reviews Molecular Cell Biology · doi:10.1038/35056563 · Cited by 1,472 · see citing works
Interferons: cell signalling, immune modulation, antiviral response and virus countermeasures
Goodbourn, S., Didcock, L., & Randall, R. E. (2000) · Journal of General Virology · doi:10.1099/0022-1317-81-10-2341 · Cited by 977 · see citing works
Self-citationThe lore of the RINGs: substrate recognition and catalysis by ubiquitin ligases
Jackson, P. K., Eldridge, A. G., Freed, E., Furstenthal, L., Hsu, J. Y., Kaiser, B. K., & Reimann, J. D. R. (2000) · Trends in Cell Biology · doi:10.1016/s0962-8924(00)01834-1 · Cited by 635 · see citing works
Didcock, L., Young, D. F., Goodbourn, S., & Randall, R. E. (1999) · Journal of Virology · doi:10.1128/jvi.73.12.9928-9933.1999 · Cited by 422 · see citing works
Self-citationCullin‐based ubiquitin ligases: Cul3–BTB complexes join the family
Pintard, L., Willems, A. R., & Peter, M. (2004) · The EMBO Journal · doi:10.1038/sj.emboj.7600186 · Cited by 413 · see citing works
Human De-Etiolated-1 Regulates c-Jun by Assembling a CUL4A Ubiquitin Ligase
Wertz, I. E., O’Rourke, K. M., Zhang, Z., Dornan, D., Arnott, D. P., Deshaies, R. J., & Dixit, V. M. (2004) · Science · doi:10.1126/science.1093549 · Cited by 366 · see citing works
Ulane, C. M., & Horvath, C. M. (2002) · Virology · doi:10.1006/viro.2002.1773 · Cited by 225 · see citing works
Parisien, J.-P., Lau, J. F., Rodriguez, J. J., Sullivan, B. M., Moscona, A., Parks, G. D., Lamb, R. A., & Horvath, C. M. (2001) · Virology · doi:10.1006/viro.2001.0856 · Cited by 220 · see citing works
Paramyxoviridae Use Distinct Virus-Specific Mechanisms to Circumvent the Interferon Response
Young, D. F., Didcock, L., Goodbourn, S., & Randall, R. E. (2000) · Virology · doi:10.1006/viro.2000.0240 · Cited by 196 · see citing works
Self-citationSTAT3 Ubiquitylation and Degradation by Mumps Virus Suppress Cytokine and Oncogene Signaling
Ulane, C. M., Rodriguez, J. J., Parisien, J.-P., & Horvath, C. M. (2003) · Journal of Virology · doi:10.1128/jvi.77.11.6385-6393.2003 · Cited by 193 · see citing works
He, B., Paterson, R. G., Stock, N., Durbin, J. E., Durbin, R. K., Goodbourn, S., Randall, R. E., & Lamb, R. A. (2002) · Virology · doi:10.1006/viro.2002.1738 · Cited by 183 · see citing works
Self-citationAndrejeva, J., Young, D. F., Goodbourn, S., & Randall, R. E. (2002) · Journal of Virology · doi:10.1128/jvi.76.5.2159-2167.2002 · Cited by 166 · see citing works
Self-citationCullin 4A Associates with the UV-damaged DNA-binding Protein DDB
Shiyanov, P. A., Nag, A., & Raychaudhuri, P. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.50.35309 · Cited by 159 · see citing works
Didcock, L., Young, D. F., Goodbourn, S., et al. (1999) · Journal of Virology · doi:10.1128/jvi.73.4.3125-3133.1999 · Cited by 152 · see citing works
Self-citationUV-damaged DNA-binding Proteins Are Targets of CUL-4A-mediated Ubiquitination and Degradation
Chen, X., Zhang, Y., Douglas, L. C., & Zhou, P. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m106808200 · Cited by 149 · see citing works
The V Protein of the Paramyxovirus SV5 Interacts with Damage-Specific DNA Binding Protein
Lin, G., Paterson, R. G., Richardson, C. D., & Lamb, R. A. (1998) · Virology · doi:10.1006/viro.1998.9317 · Cited by 137 · see citing works
Parisien, J.-P., Lau, J. F., Rodriguez, J. J., Ulane, C. M., & Horvath, C. M. (2002) · Journal of Virology · doi:10.1128/jvi.76.9.4190-4198.2002 · Cited by 136 · see citing works
Parisien, J.-P., Lau, J. F., & Horvath, C. M. (2002) · Journal of Virology · doi:10.1128/jvi.76.13.6435-6441.2002 · Cited by 107 · see citing works
Andrejeva, J., Poole, E. L., Young, D. F., Goodbourn, S., & Randall, R. E. (2002) · Journal of Virology · doi:10.1128/jvi.76.22.11379-11386.2002 · Cited by 106 · see citing works
Self-citationSTAT1 Is Inactivated by a Caspase
King, P., & Goodbourn, S. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.15.8699 · Cited by 97 · see citing works
Self-citationSilencing STATs: lessons from paramyxovirus interferon evasion
Horvath, C. M. (2004) · Cytokine & Growth Factor Reviews · doi:10.1016/j.cytogfr.2004.02.003 · Cited by 95 · see citing works
Young, D. F., Chatziandreou, N., He, B., et al. (2001) · Journal of Virology · doi:10.1128/jvi.75.7.3363-3370.2001 · Cited by 91 · see citing works
Self-citationNishio, M., Tsurudome, M., Ito, M., et al. (2001) · Journal of Virology · doi:10.1128/jvi.75.19.9165-9176.2001 · Cited by 78 · see citing works
Yokosawa, N., Yokota, S., Kubota, T., et al. (2002) · Journal of Virology · doi:10.1128/jvi.76.24.12683-12690.2002 · Cited by 75 · see citing works
García‐Sastre, A. (2004) · Current topics in microbiology and immunology · doi:10.1007/978-3-662-06099-5_7 · Cited by 68 · see citing works
Chatziandreou, N., Young, D. F., Andrejeva, J., Goodbourn, S., & Randall, R. E. (2002) · Virology · doi:10.1006/viro.2001.1302 · Cited by 59 · see citing works
Self-citationLeupin, O., Bontron, S., & Strubin, M. (2003) · Journal of Virology · doi:10.1128/jvi.77.11.6274-6283.2003 · Cited by 52 · see citing works
Nagai, Y., & Kato, A. (2004) · Current topics in microbiology and immunology · doi:10.1007/978-3-662-06099-5_6 · Cited by 49 · see citing works
Randall, R. E., & Young, D. F. (1991) · Journal of Virology · doi:10.1128/jvi.65.2.719-726.1991 · Cited by 26 · see citing works
Self-citation
Flagged first, then by citations
Cited by
Sorted by citations
Chasing molecular glue degraders: screening approaches
Domostegui, A., Nieto-Barrado, L., Perez-Lopez, C., & Mayor-Ruiz, C. (2022) · Chemical Society Reviews · doi:10.1039/d2cs00197g · Cited by 200 · see citing works
Precious, B., Childs, K., Fitzpatrick-Swallow, V., Goodbourn, S., & Randall, R. E. (2005) · Journal of Virology · doi:10.1128/jvi.79.21.13434-13441.2005 · Cited by 117 · see citing works
Self-citationCullin E3 Ligases and Their Rewiring by Viral Factors
Mahon, C., Krogan, N., Craik, C., & Pick, E. (2014) · Biomolecules · doi:10.3390/biom4040897 · Cited by 101 · see citing works
- Authors
- Precious, B., Young, D. F., Andrejeva, L., Goodbourn, S., & Randall, R. E.
- Affiliations
- University of St Andrews (GB)
- University of London (GB)
- Venue
- Journal of General Virology
- Publisher
- Microbiology Society
- Published
- 2005-01-01
- Volume
- 86
- Issue
- 1
- Pages
- 151-158
- ISSN
- 0022-1317 (ISSN-L)
- 1465-2099
- OpenAlex
- W2132148594
- Cited by
- 56
- References
- 30
- Citations by year
- 2026: 3 · 2025: 1 · 2024: 1 · 2023: 1 · 2022: 5 · 2021: 1 · 2020: 1 · 2019: 2 · 2018: 1 · 2017: 2 · 2016: 2 · 2015: 2 · 2014: 5 · 2013: 4 · 2012: 2
- FWCI
- 2.87
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of General Virology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Wellcome Trust
- Biotechnology and Biological Sciences Research Council
- Topics
- Health Sciences › Medicine › Epidemiology › Virology and Viral Diseases
- Life Sciences › Immunology and Microbiology › Immunology › interferon and immune responses
- Health Sciences › Medicine › Oncology › Cytokine Signaling Pathways and Interactions
- Keywords
- STAT1, STAT2, simian virus 5, human parainfluenza virus type 2, paramyxovirus, ubiquitination, proteasomal degradation, polyubiquitination, interferon signaling
- MeSH terms
- Animals, Cell Line, DNA-Binding Proteins, Humans, Rabbits, Reticulocytes, Species Specificity, Viral Proteins, Signal Transduction, Trans-Activators, Viral Structural Proteins, Respirovirus, Ubiquitin, STAT1 Transcription Factor, STAT2 Transcription Factor
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
