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PreprintCited by 1Bacterial Genetics and BiotechnologyBacteriophages and microbial interactionsGenomics and Phylogenetic Studies

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Prophage-encoded small protein YqaH counteracts the activities of the replication initiator DnaA in Bacillus subtilis

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Ventroux, Magali, Noirot-Gros, Marie-Francoise·2020·Microbiology
DOI 10.1101/2020.11.18.388090

Preprint — published as 10.1099/mic.0.001268 · Paper page

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Abstract

Bacterial genomes harbor cryptic prophages that are mostly transcriptionally silent with many unannotated genes. Still, cryptic prophages may contribute to their host fitness and phenotypes. In B. subtilis , the yqaF-yqaN operon belongs to the prophage element skin , and is tightly repressed by the Xre-like repressor sknR . This operon contains several short open reading frames (smORFs) potentially encoding small-sized proteins. The smORF-encoded peptide YqaH was previously reported to bind to the replication initiator DnaA. Here, using a yeast two-hybrid assay, we found that YqaH binds to the DNA binding domain IV of DnaA and interacts with Spo0A, a master regulator of sporulation. We isolated single amino acid substitutions in YqaH that abolished interaction with DnaA but not with Spo0A. Then, we studied in B. subtilis the phenotypes associated with the specific loss-of-interaction with DnaA (DnaA-LOI). We found that expression of yqaH carrying DnaA-LOI mutations abolished the deleterious effects of yqaH WT expression on chromosome segregation, replication initiation and DnaA-regulated transcription. When YqaH was induced after vegetative growth, DnaA-LOI mutations abolished the deleterious effects of YqaH WT on sporulation and biofilm formation. Thus, YqaH inhibits replication, sporulation and biofilm formation mainly by antagonizing DnaA in a manner that is independent of the cell cycle checkpoint Sda.

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Metadata

Authors
Ventroux, M., & Noirot-Gros, M.-F.
Affiliations
AgroParisTech (FR)
ORCID
Ventroux, Magali
Venue
Microbiology
Publisher
openRxiv
Published
2020-11-18
Volume
168
Issue
11
Pages
001268
ISSN
1350-0872 (ISSN-L)
DOI
10.1101/2020.11.18.388090
OpenAlex
W4310359231
Cited by
1
References
1
Citations by year
2024: 1
FWCI
0.15
Type
journal article
Language
en
Open access
Licence
Locations
  • Microbiology (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
  • HAL (submittedVersion, other-oa)
Funders
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Immunology and Microbiology › Virology › Bacteriophages and microbial interactions
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
Keywords
cryptic prophage, DnaA, Bacillus subtilis, Spo0A, sporulation, biofilm formation, chromosome segregation, DNA replication initiation, protein-protein interaction, transcriptional repression
MeSH terms
Bacillus subtilis, Bacterial Proteins, DNA Replication, DNA-Binding Proteins, Transcription Factors, Prophages

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