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PreprintCited by 26Insect symbiosis and bacterial influencesPhytoplasmas and Hemiptera pathogensInvertebrate Immune Response Mechanisms

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Dual proteomics of Drosophila melanogaster hemolymph infected with the heritable endosymbiont Spiroplasma poulsonii

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Masson, Florent, Rommelaere, Samuel, Marra, Alice, Schüpfer, Fanny, Lemaitre, Bruno·2021·PLoS ONE
DOI 10.1101/2021.02.22.432267PMC PMC8084229

Preprint — published as 10.1371/journal.pone.0250524 · Paper page

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Abstract

Insects are frequently infected with heritable bacterial endosymbionts. Endosymbionts have a dramatic impact on their host physiology and evolution. Their tissue distribution is variable with some species being housed intracellularly, some extracellularly and some having a mixed lifestyle. The impact of extracellular endosymbionts on the biofluids they colonize (e.g. insect hemolymph) is however difficult to appreciate because biofluids composition can depend on the contribution of numerous tissues. Here we address the question of Drosophila hemolymph proteome response to the infection with the endosymbiont Spiroplasma poulsonii. S. poulsonii inhabits the fly hemolymph and gets vertically transmitted over generations by hijacking the oogenesis in females. Using dual proteomics on infected hemolymph, we uncovered a low level and chronic activation of the Toll immune pathway by S. poulsonii that was previously undetected by transcriptomics-based approaches. Using Drosophila genetics, we also identified candidate proteins putatively involved in controlling S. poulsonii growth. Last, we also provide a deep proteome of S. poulsonii , which, in combination with previously published transcriptomics data, improves our understanding of the post-transcriptional regulations operating in this bacterium. Summary statement: We report the changes in Drosophila melanogaster hemolymph proteome upon infection with the heritable bacterial endosymbiont Spiroplasma poulsonii .

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Metadata

Authors
Masson, F., Rommelaere, S., Marra, A., Schüpfer, F., & Lemaitre, B.
Affiliations
École Polytechnique Fédérale de Lausanne (CH)
ORCID
Venue
PLoS ONE
Publisher
openRxiv
Published
2021-02-22
Volume
16
Issue
4
Pages
e0250524-e0250524
ISSN
1932-6203 (ISSN-L)
DOI
10.1101/2021.02.22.432267
PMC
PMC8084229
OpenAlex
W3157407611
Cited by
26
References
1
Citations by year
2026: 2 · 2025: 7 · 2024: 6 · 2023: 6 · 2022: 3 · 2021: 2
FWCI
2.92
Type
journal article
Language
en
Open access
Licence
Locations
  • PLoS ONE (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion, cc-by-nc-nd)
Funders
  • National Science Foundation
  • École Polytechnique Fédérale de Lausanne
  • Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (185295)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Insect Science › Insect symbiosis and bacterial influences
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Phytoplasmas and Hemiptera pathogens
  • Life Sciences › Immunology and Microbiology › Immunology › Invertebrate Immune Response Mechanisms
Keywords
Drosophila melanogaster, hemolymph proteome, endosymbiotic bacteria, host immune response, vertical transmission, post-transcriptional regulation, host-symbiont interactions
MeSH terms
Animals, Bacterial Proteins, Drosophila melanogaster, Female, Hemolymph, Oogenesis, Spiroplasma, Symbiosis, Signal Transduction, Proteome, Proteomics

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