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Multiple classes and isoforms of the RNA polymerase recycling motor protein HelD
Open accessPreprint — published as 10.1002/mbo3.1251
Abstract
SUMMARY: Efficient control of transcription is essential in all organisms. In bacteria, where DNA replication and transcription occur simultaneously, the replication machinery is at risk of colliding with highly abundant transcription complexes. This can be exacerbated by the fact that transcription complexes pause frequently. When pauses are long-lasting, the stalled complexes must be removed to prevent collisions with either another transcription complex or the replication machinery. HelD is a protein that represents a new class of ATP-dependent motor protein distantly related to helicases. It was first identified in the model Gram-positive bacterium Bacillus subtilis and is involved in removing and recycling stalled transcription complexes. To date, two classes of HelD have been identified: one in the low G+C and the other in the high G+C Gram-positive bacteria. In this work we have undertaken the first comprehensive investigation of the phylogenetic diversity of HelD proteins. We show that genes in certain bacterial classes have been inherited by horizontal gene transfer, many organisms contain multiple expressed isoforms of HelD, some of which are associated with antibiotic resistance, and that there is a third class of HelD protein found in Gram-negative bacteria. Therefore, HelD proteins represent an important new class of transcription factor associated with genome maintenance and antibiotic resistance that are conserved across the Eubacterial kingdom.
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Metadata
- Authors
- Larsen, J. S., Miller, M., Oakley, A. J., Dixon, N. E., & Lewis, P. J.
- Affiliations
- University of Newcastle Australia (AU)
- University of Wollongong (AU)
- Corresponding author
- Lewis, Peter J.
- Venue
- MicrobiologyOpen
- Publisher
- openRxiv
- Published
- 2021-08-19
- Volume
- 10
- Issue
- 6
- Pages
- e1251-e1251
- ISSN
- 2045-8827 (ISSN-L)
- PMC
- PMC8655204
- OpenAlex
- W4232728066
- Cited by
- 7
- References
- 1
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 1 · 2023: 1 · 2022: 1 · 2021: 1
- FWCI
- 0.56
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- MicrobiologyOpen (publishedVersion, cc-by)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Research Online (University of Wollongong) (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Figshare (submittedVersion)
- NOVA (University of Newcastle Australia) (submittedVersion)
- Funders
- Australian Research Council (DP210100365)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Keywords
- HelD, transcription termination, horizontal gene transfer, antibiotic resistance, genome maintenance, Bacillus subtilis, phylogenetic diversity, Gram-positive bacteria, Gram-negative bacteria
- MeSH terms
- Protein Domains, Bacteria, Bacterial Proteins, DNA Helicases, Models, Molecular, Phylogeny, DNA-Directed RNA Polymerases, Transcription Factors, Transcription, Genetic, Protein Isoforms, Gene Transfer, Horizontal
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