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PreprintCited by 0RNA Research and SplicingCellular transport and secretionFungal and yeast genetics research

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A MademoiseLLE domain binding platform links the key RNA transporter to endosomes

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Devan, Senthil-Kumar, Schott-Verdugo, Stephan, Müntjes, Kira, Bismar, Lilli, Reiners, Jens, Hachani, Eymen, Schmitt, Lutz, Höppner, Astrid, Smits, Sander HJ, Gohlke, Holger, Feldbrügge, Michael·2021·bioRxiv (Cold Spring Harbor Laboratory)
DOI 10.1101/2021.10.25.465676

Preprint — published as 10.1371/journal.pgen.1010269 · Paper page

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Abstract

Spatiotemporal expression can be achieved by transport and translation of mRNAs at defined subcellular sites. An emerging mechanism mediating mRNA trafficking is microtubule- dependent co-transport on shuttling endosomes. Although progress has been made in identifying various components of the endosomal mRNA transport machinery, a mechanistic understanding of how these RNA-binding proteins are connected to endosomes is still lacking. Here, we demonstrate that a flexible MademoiseLLE (MLLE) domain platform within RNA- binding protein Rrm4 of Ustilago maydis is crucial for endosomal attachment. Our structure/function analysis uncovered three MLLE domains at the C-terminus of Rrm4 with a functionally defined hierarchy. MLLE3 recognises two PAM2-like sequences of the adaptor protein Upa1 and is essential for endosomal shuttling of Rrm4. MLLE1 and MLLE2 are most likely accessory domains exhibiting a variable binding mode for interaction with currently unknown partners. Thus, endosomal attachment of the mRNA transporter is orchestrated by a sophisticated MLLE domain binding platform.

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Research works printed but not linked: 69

  1. 10.1021/bi4008116 · DOI
  2. 10.1107/S0907444912001308 · DOI · Paper page
  3. 10.1016/j.bbrc.2004.02.024 · DOI
  4. 10.1126/science.abj8754 · DOI · Paper page
  5. 10.1002/embr.201338037 · DOI
  6. 10.1242/jcs.101212 · DOI
  7. Baumann S , Zander S , Weidtkamp-Peters S , Feldbrügge M (2016) Live cell imaging of septin dynamics in Ustilago maydis . In: Methods in Cell Biol: Septins, Gladfelter A.S . (ed.) pp. 143–159. Elsevier Inc.: · DOI
  8. 10.1242/jcs.03287 · DOI
  9. 10.1016/j.tcb.2018.10.005 · DOI
  10. 2004, A reverse genetic approach for generating gene replacement mutants in Ustilago maydis, Mol Gen Genom, 272, 216, 10.1007/s00438-004-1047-z · DOI
  11. 10.1046/j.1365-2958.2001.02699.x · DOI
  12. 10.1016/j.cell.2018.11.030 · DOI
  13. 10.1038/s41580-021-00356-8 · DOI
  14. 10.1073/pnas.071552198 · DOI
  15. 10.1107/S0907444904019158 · DOI · Paper page
  16. Fernandopulle MS , Lippincott-Schwartz J , Ward ME (2021) RNA transport and local translation in neurodevelopmental and neurodegenerative disease. Nature neuroscience · DOI · Paper page
  17. 10.1107/S1600576717007786 · DOI
  18. 10.1107/S0021889809000338 · DOI · Paper page
  19. Grimm C , Pelz JP , Schneider C , Schaffler K , Fischer U (2020) Crystal structure of a variant PAM2 motif of LARP4B bound to the MLLE domain of PABPC1. Biomol 10 · DOI
  20. 10.1146/annurev-micro-091014-104242 · DOI
  21. 2019, The multi PAM2 protein Upa2 functions as novel core component of endosomal mRNA transport, EMBO Rep, 24, e47381
  22. 10.1038/nsmb.1768 · DOI
  23. 10.1107/S1399004714013534 · DOI
  24. 10.1002/pro.3731 · DOI
  25. 10.1107/S0021889803012779 · DOI
  26. 10.1038/sj.emboj.7600048 · DOI
  27. 10.1371/journal.pone.0010169 · DOI
  28. 10.1016/j.jmb.2010.01.032 · DOI
  29. 10.1073/pnas.071024998 · DOI
  30. 10.1016/j.cell.2019.08.050 · DOI · Paper page
  31. 10.1074/jbc.M600307200 · DOI
  32. 10.1046/j.1365-2958.2001.02424.x · DOI
  33. Manalastas-Cantos K , Konarev PV , Hajizadeh NR , Kikhney AG , Petoukhov MV , Molodenskiy DS , Panjkovich A , Mertens HDT , Gruzinov A , Borges C et al. (2021) ATSAS 3.0: expanded functionality and new tools for small-angle scattering data analysis. J Appl Crystallogr 54 · DOI
  34. 10.1128/MCB.18.12.7383 · DOI
  35. 10.1186/gb-2003-4-7-223 · DOI
  36. 2021, LARP1 and LARP4: up close with PABP for mRNA 3’ poly(A) protection and stabilization, RNA Biol, 18, 259, 10.1080/15476286.2020.1868753 · DOI
  37. 2017, SnapShot: Subcellular mRNA localization, Cell, 169, e171
  38. 2019, Core components of endosomal mRNA transport are evolutionarily conserved in fungi, Fungal Genet Biol, 126, 12, 10.1016/j.fgb.2019.01.013 · DOI
  39. 10.1021/acs.jctc.8b00690 · DOI
  40. 2021, TopSuite Web Server: A Meta-Suite for Deep-Learning-Based Protein Structure and Quality Prediction, Journal of chemical information and modeling, 61, 548, 10.1021/acs.jcim.0c01202 · DOI
  41. 10.1021/acs.jctc.9b00825 · DOI
  42. 10.1021/acs.jctc.9b00825 · DOI
  43. 10.1074/jbc.M115.672246 · DOI
  44. Müntjes K , Devan SK , Reichert AS , Feldbrügge M (2021) Linking transport and translation of mRNAs with endosomes and mitochondria. EMBO reports accepted for publication · DOI
  45. 2020, Establishing polycistronic expression in the model microorganism Ustilago maydis, Front Microbiol, 11, 1384, 10.3389/fmicb.2020.01384 · DOI
  46. 2018, mRNA transport in fungal top models, Wiley interdisciplinary reviews RNA, 9, e1453, 10.1002/wrna.1453 · DOI
  47. 10.15252/embr.201846588 · DOI
  48. 10.1107/S0909049513010431 · DOI
  49. 2010, New beamline dedicated to solution scattering from biological macromolecules at the ESRF, Journal of Physics: Conference Series, 247, 012009
  50. 10.7554/eLife.06041 · DOI
  51. Quentin D , Schuhmacher JS , Klink BU , Lauer J , Shaikh TR , Huis in t’t Veld PJ , Welp LM , Urlaub H , Zerial M , Raunser S (2021) Structure of the human FERRY Rab5 effector complex. bioRiv 10.1101/2021.06.21.449265 · DOI
  52. 2018, Characterization of the multimeric structure of poly(A)-binding protein on a poly(A) tail, Sci Rep, 8, 1455, 10.1038/s41598-018-19659-6 · DOI
  53. 10.1016/j.cell.2019.04.013 · DOI
  54. 10.1101/2021.06.20.449167 · DOI
  55. 10.1016/j.ymeth.2008.06.012 · DOI
  56. 10.1016/S0014-5793(01)03308-7 · DOI · Paper page
  57. 10.1016/j.pep.2005.01.016 · DOI
  58. 10.1016/j.molcel.2011.06.013 · DOI
  59. 10.1107/S0021889892001663 · DOI
  60. 10.1016/j.fgb.2013.10.012 · DOI
  61. 10.1104/pp.19.00972 · DOI
  62. 10.1105/tpc.18.00321 · DOI
  63. 10.1105/tpc.20.00111 · DOI
  64. 10.1107/S205225251500202X · DOI
  65. 10.1093/nar/gku1047 · DOI
  66. Weiler AJ , Spitz O , Gudzuhn M , Schott-Verdugo SN , Kamel M , Thiele B , Streit RW , Kedrov A, L. S , Gohlke H et al. (2021) Novel intracellular phospholipase B from Pseudomonas aeruginosa with activity towards endogenous phospholipids affects biofilm assembly. bioRiv doi.org/10.1101/2021.06.15.448513 · DOI
  67. 10.1016/j.bbagrm.2014.08.001 · DOI
  68. 10.1128/MCB.01162-10 · DOI
  69. 10.1242/jcs.182824 · DOI

Metadata

Authors
Devan, S.-K., Schott-Verdugo, S., Müntjes, K., Bismar, L., Reiners, J., Hachani, E., Schmitt, L., Höppner, A., Smits, S. H., Gohlke, H., & Feldbrügge, M.
Affiliations
  • Cluster of Excellence on Plant Sciences (DE)
  • Heinrich Heine University Düsseldorf (DE)
  • Forschungszentrum Jülich (DE)
Corresponding author
  • Smits, Sander HJ
  • Gohlke, Holger
  • Feldbrügge, Michael
ORCID
Venue
bioRxiv (Cold Spring Harbor Laboratory)
Publisher
openRxiv
Published
2021-10-25
DOI
10.1101/2021.10.25.465676
OpenAlex
W3211005898
Cited by
0
References
1
Type
posted content
Language
en
Open access
Licence
cc-by-nc
Locations
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc)
  • Desy Publications Database (Deutsches Elektronen-Synchrotron DESY) (submittedVersion, other-oa)
Funders
  • Deutsche Forschungsgemeinschaft (project A01), SFB 1208, Project A03, EXC-2048, EXC-2048/1, 267205415, INST 208/740‐1 FUGG, INST 208/761‐1 FUGG, 39068111, 417919780, 2048/1)
  • European Synchrotron Radiation Facility
  • Heinrich-Heine-Universität Düsseldorf
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
Keywords
RNA-binding proteins, Ustilago maydis, structure-function analysis

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