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Identification of putative reader proteins of 5-methylcytosine and its derivatives in Caenorhabditis elegans RNA
Open accessPreprint — published as 10.12688/wellcomeopenres.17893.1
Abstract
Methylation of carbon-5 of cytosines (m 5 C) is a post-transcriptional nucleotide modification of RNA with widespread distribution across organisms. m 5 C has been shown to participate in mRNA transport and maintain mRNA stability through its recognition by the reader proteins ALYREF and YBX1, respectively. We recently showed that m 5 C is required for Caenorhabditis elegans development and fertility under heat stress. To contribute to the understanding of how m 5 C and its oxidative derivatives mediate their functions, we developed RNA baits bearing modified cytosines in diverse structural contexts to pulldown potential readers in C. elegans . Our mass spectrometry analyses reveal unique binding proteins for each of the modifications. We validate our dataset by demonstrating that the nematode ALYREF homologues ALY-1 and ALY-2 preferentially bind m 5 C in vitro . The dataset presented here serves as an important scientific resource that will support the discovery of new functions of m 5 C and its derivatives.
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Global quantification of mammalian gene expression control
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Metadata
- Authors
- Navarro, I. C., Suen, K. M., Bensaddek, D., Tanpure, A., Lamond, A., Balasubramanian, S., & Miska, E. A.
- Affiliations
- University of Southern California (US)
- University of Dundee (GB)
- University of Cambridge (GB)
- Corresponding author
- Miska, Eric A.
- ORCID
- Venue
- Wellcome Open Research
- Publisher
- openRxiv
- Published
- 2022-01-25
- Volume
- 7
- Pages
- 282-282
- ISSN
- 2398-502X (ISSN-L)
- PMC
- PMC10354459
- OpenAlex
- W4309218440
- Cited by
- 3
- References
- 1
- Citations by year
- 2024: 2 · 2022: 1
- FWCI
- 0.24
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- Wellcome Open Research (publishedVersion, cc-by)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Discovery Research Portal (University of Dundee) (acceptedVersion, public-domain)
- White Rose Research Online (University of Leeds, The University of Sheffield, University of York) (acceptedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- King Abdullah University of Science and Technology Repository (King Abdullah University of Science and Technology) (submittedVersion, cc-by)
- Funders
- Wellcome Trust (104640/Z/14/Z, 092096/Z/10/Z, Z/17/Z, C6946/A24843, 205589/2014-6, 203144, 104640, 092096, 209441, 209441/Z/17/Z, 203144/Z/16/Z)
- Cancer Research UK (A27826, C9545/A19836, 092096/Z/10/Z, C9681/A29214, C6946/A24843, 19836, C13474, C9545, 092096, C6946, A24843, 27826, C13474/A27826)
- Conselho Nacional de Desenvolvimento Científico e Tecnológico (P40 OD010440, 2014-6, 205589/2014‐6)
- National Institutes of Health (OD010440, P40OD010440)
- Biotechnology and Biological Sciences Research Council (BB/V010948/1)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA modifications and cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
- Keywords
- 5-methylcytosine, RNA methylation, ALYREF, RNA recognition motif, Caenorhabditis elegans, germ cell development, RNA processing
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