CoChat
Sign Up
PreprintCited by 19RNA and protein synthesis mechanismsPlant-Microbe Interactions and ImmunityRNA modifications and cancer

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

The tRNA thiolation-mediated translational control is essential for plant immunity

Open access
Zheng, Xueao, Chen, Hanchen, Deng, Zhiping, Wu, Yujing, Zhong, Linlin, Wu, Chong, Chen, Qiansi, Yan, Shunping·2022·eLife
DOI 10.1101/2022.02.13.480182PMC PMC10863982

Preprint — published as 10.7554/elife.93517 · Paper page

Open this paper in CoChat — free account

Abstract

Plants have evolved sophisticated mechanisms to regulate gene expression to activate immune responses against pathogen infections. However, how the translation system contributes to plant immunity is largely unknown. The evolutionarily conserved thiolation modification of tRNA ensures efficient decoding during translation. Here we show that tRNA thiolation is required for plant immunity in Arabidopsis . We identify a cgb Arabidopsis mutant, which is hyper-susceptible to the pathogen Pseudomonas syringae. CGB encodes ROL5, a homolog of yeast NCS6 required for tRNA thiolation. ROL5 physically interacts with CTU2, a homolog of yeast NCS2. Mutations in either ROL5 or CTU2 result in loss of tRNA thiolation. Further analyses reveal that both transcriptional reprogramming and translational reprogramming during immune responses are compromised in cgb. Notably, the translation of the salicylic acid receptor NPR1 is reduced in cgb , resulting in reduced salicylic acid signaling. Our study not only reveals a new regulatory mechanism for plant immunity but also uncovers a new biological function of tRNA thiolation.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1101/2022.02.13.480182

Cites

1 of 1 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Flagged first, then by citations

Research works printed but not linked: 60

  1. 10.1080/15476286.2017.1356562 · DOI
  2. 10.1016/S0092-8674(00)81858-9 · DOI
  3. 2021, RAD51 supports DMC1 by inhibiting the SMC5/6 complex during meiosis, Plant Cell, 33, 2869, 10.1093/plcell/koab136 · DOI
  4. 10.1104/pp.107.111740 · DOI
  5. 2023, Global translational induction during NLR-mediated immunity in plants is dynamically regulated by CDC123, an ATP-sensitive protein, Cell Host Microbe, 31, 334, 10.1016/j.chom.2023.01.014 · DOI
  6. 10.1146/annurev.phyto.050908.135202 · DOI · Paper page
  7. 10.1084/jem.20160397 · DOI
  8. 10.1074/mcp.M700123-MCP200 · DOI
  9. 10.1073/pnas.0709404105 · DOI
  10. 10.1016/j.cell.2018.03.044 · DOI
  11. 10.1038/nature11162 · DOI
  12. 2016, Super-resolution ribosome profiling reveals unannotated translation events in Arabidopsis, Proc. Natl. Acad. Sci. U. S. A, 113, 7126
  13. 10.1261/rna.7247705 · DOI
  14. 10.1111/j.1365-2958.2005.04972.x · DOI
  15. 10.1038/nature05286 · DOI · Paper page
  16. 10.1080/15476286.2016.1267098 · DOI
  17. 2019, Arabidopsis mlo3 mutant plants exhibit spontaneous callose deposition and signs of early leaf senescence, Plant Mol. Biol, 101, 21, 10.1007/s11103-019-00877-z · DOI
  18. 2017, Absolute quantification of protein and mRNA abundances demonstrate variability in gene-specific translation efficiency in Yeast, Cell Syst, 4, 495, 10.1016/j.cels.2017.03.003 · DOI · Paper page
  19. 10.1105/tpc.109.073007 · DOI
  20. 10.1038/nature07643 · DOI
  21. 10.1186/s13059-014-0550-8 · DOI · Paper page
  22. 10.1261/rna.2172105 · DOI
  23. 2019, tRNA wobble modification affects leaf cell development in Arabidopsis thaliana, Plant Cell Physiol, 60, 2026, 10.1093/pcp/pcz064 · DOI
  24. 10.1074/jbc.M312448200 · DOI
  25. 10.1016/j.cell.2015.05.022 · DOI
  26. 10.1093/nar/gkn1023 · DOI
  27. 2021, A novel WEE1 pathway for replication stress responses, Nat. Plants, 7, 209, 10.1038/s41477-021-00855-8 · DOI
  28. 10.1038/s41422-018-0013-y · DOI
  29. 10.1146/annurev-arplant-081320-092855 · DOI
  30. 10.1186/1471-2229-14-1 · DOI
  31. 10.1021/jacs.7b00727 · DOI
  32. 10.1126/science.aaw1720 · DOI
  33. 10.1073/pnas.1300781110 · DOI
  34. 10.1016/j.chom.2015.07.005 · DOI
  35. 10.1080/15476286.2017.1295204 · DOI
  36. 10.1093/nar/3.3.523 · DOI
  37. 2019, Biallelic variants in CTU2 cause DREAM-PL syndrome and impair thiolation of tRNA wobble U34, Hum. Mutat, 40, 2108, 10.1002/humu.23870 · DOI
  38. 10.1093/hmg/ddn375 · DOI
  39. 10.1038/nri3141 · DOI
  40. 10.1016/j.cell.2009.03.038 · DOI
  41. 10.1038/nprot.2014.047 · DOI
  42. 10.1126/science.1156970 · DOI
  43. 10.1016/j.molmed.2014.01.008 · DOI
  44. 2022, PABP/purine-rich motif as an initiation module for cap-independent translation in pattern-triggered immunity, Cell, 185, 3186, 10.1016/j.cell.2022.06.037 · DOI
  45. 10.1186/s13059-015-0715-0 · DOI
  46. 10.1038/s41586-020-2164-5 · DOI
  47. 10.1016/j.celrep.2012.05.008 · DOI
  48. 10.1038/nature22372 · DOI
  49. 2020, Natural variations of SLG1 confer high-temperature tolerance in indica rice, Nat. Commun, 11, 5441, 10.1038/s41467-020-19320-9 · DOI
  50. 10.1016/j.molcel.2013.09.019 · DOI
  51. 10.1016/j.pbi.2014.04.006 · DOI
  52. 2019, Translational regulation of metabolic dynamics during effector-triggered immunity, Mol. Plant, 13, 88
  53. 2012, ClusterProfiler: An R package for comparing biological themes among gene clusters, Omi. A J. Integr. Biol, 16, 284, 10.1089/omi.2011.0118 · DOI · Paper page
  54. Yu, X. , Cui, X. , Wu, C. , Shi, S. , and Yan, S . (2022). Salicylic acid inhibits gibberellin signaling through receptor interactions. Mol. Plant. · DOI
  55. 2021, Salicylic acid and ethylene coordinately promote leaf senescence, J. Integr. Plant Biol, 63, 823, 10.1111/jipb.13074 · DOI
  56. 10.1111/j.1365-2958.2008.06273.x · DOI
  57. 2020, Formation of NPR1 condensates promotes cell survival during the plant immune response, Cell, 182, 1093, 10.1016/j.cell.2020.07.016 · DOI
  58. 10.1016/j.molp.2020.08.001 · DOI
  59. 10.1016/j.cell.2020.04.028 · DOI
  60. 10.1371/journal.pgen.1003675 · DOI

Metadata

Authors
Zheng, X., Chen, H., Deng, Z., Wu, Y., Zhong, L., Wu, C., Chen, Q., & Yan, S.
Affiliations
  • Huazhong Agricultural University (CN)
  • ZheJiang Academy of Agricultural Sciences (CN)
  • China Tobacco (CN)
Corresponding author
Yan, Shunping
ORCID
Venue
eLife
Publisher
openRxiv
Published
2022-02-14
Volume
13
ISSN
2050-084X (ISSN-L)
DOI
10.1101/2022.02.13.480182
PMC
PMC10863982
OpenAlex
W4391322744
Cited by
19
References
1
Citations by year
2026: 8 · 2025: 9 · 2024: 2
FWCI
2.58
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • eLife (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • National Natural Science Foundation of China (31970311, 32270306, 32000373)
  • Huazhong Agricultural University
  • Recruitment Program of Global Experts
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant-Microbe Interactions and Immunity
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA modifications and cancer
Keywords
tRNA thiolation, plant immunity, translational regulation, Pseudomonas syringae, salicylic acid signaling, Arabidopsis thaliana
MeSH terms
Mutation, Plant Diseases, RNA, Transfer, Saccharomyces cerevisiae, Arabidopsis, Gene Expression Regulation, Plant, Salicylic Acid, Arabidopsis Proteins, Plant Immunity

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review