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PreprintCited by 29Plant Molecular Biology ResearchPlant biochemistry and biosynthesisPlant Parasitism and Resistance

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Chemical genetic screening identifies nalacin as an inhibitor of GH3 amido synthetase for auxin conjugation

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Xie, Yinpeng, Zhu, Ying, Wang, Na, Luo, Min, Ohta, Tsuyoshi, Guo, Ruipan, Yu, Zongjun, Aizezi, Yalikunjiang, Zhang, Linlin, Yan, Yan, Zhang, Yujie, Bao, Hongyu, Wang, Yichuan, Zhu, Ziqiang, Huang, Ancheng, Zhao, Yunde, Asami, Tadao, Huang, Hongda, Guo, Hongwei, Jiang, Kai·2022·Proceedings of the National Academy of Sciences
DOI 10.1101/2022.05.29.493926PMC PMC9894192

Preprint — published as 10.1073/pnas.2209256119 · Paper page

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Abstract

Root system is critical for plant growth and development. To develop plant growth regulators functioning in root development, we performed a phenotype-based chemical screen in Arabidopsis and identified a chemical, nalacin, that mimicked the effects of auxin on root development. Genetic, pharmacological and biochemical approaches demonstrated that nalacin exerts its auxin-like activities by inhibiting indole-3-acetic acid (IAA) conjugation that is mediated by Gretchen Hagen 3 (GH3) acyl acid amido synthetases. The crystal structure of Arabidopsis GH3.6 in complex with D4 (a derivative of nalacin) together with docking simulation analysis revealed the molecular basis of the inhibition of group II GH3 by nalacin. Sequence alignment analysis indicated broad bioactivities of nalacin and D4 as inhibitors of GH3s in vascular plants, which were confirmed, at least, in tomato and rice. In summary, our work identifies nalacin as a potent inhibitor of IAA conjugation mediated by group II GH3 that plays versatile roles in hormone-regulated plant development and has potential applications in both basic research and agriculture.

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Research works printed but not linked: 54

  1. 10.1101/cshperspect.a039933 · DOI
  2. 10.3389/fpls.2016.01509 · DOI
  3. 10.1101/cshperspect.a001537 · DOI
  4. 10.1242/dev.086363 · DOI
  5. 10.1199/tab.0173 · DOI
  6. 10.1101/cshperspect.a039867 · DOI
  7. 10.1101/gad.1415106 · DOI
  8. 10.1016/j.cell.2008.01.047 · DOI
  9. 10.1073/pnas.1613506113 · DOI
  10. 10.1016/j.devcel.2013.09.005 · DOI
  11. 10.1073/pnas.1604375113 · DOI
  12. 10.1073/pnas.1604769113 · DOI
  13. 2021, The main oxidative inactivation pathway of the plant hormone auxin, Nat. Commun., 12, 6752, 10.1038/s41467-021-27020-1 · DOI
  14. 10.1105/tpc.000885 · DOI
  15. 10.1105/tpc.104.026690 · DOI
  16. 10.1007/s11103-011-9776-y · DOI
  17. 10.1073/pnas.1612635113 · DOI
  18. 10.1105/tpc.104.023549 · DOI
  19. 10.1074/jbc.RA119.009949 · DOI
  20. 10.1126/science.aaw1720 · DOI
  21. 10.1074/jbc.RA118.004975 · DOI
  22. 10.1074/jbc.RA118.002006 · DOI
  23. 10.1104/pp.103.031138 · DOI
  24. 10.1146/annurev-arplant-042811-105456 · DOI
  25. 2018, Chemical regulators of plant hormones and their applications in basic research and agriculture, Biosci. Biotechnol. Biochem., 8451, 1
  26. 2018, Manipulation and sensing of auxin metabolism, transport and signaling, Plant Cell Physiol., 59, 1500, 10.1093/pcp/pcy076 · DOI
  27. 10.1371/journal.pone.0035495 · DOI
  28. K. Fukui , et al., Chemical inhibition of auxin inactivation pathway uncovers the metabolic turnover of auxin homeostasis. bioRxiv, https:/doi.org/10.1101/2021.10.12.464031. (2021) · DOI
  29. 10.1073/pnas.0437936100 · DOI
  30. 10.1038/nature10791 · DOI · Paper page
  31. 10.1128/MCB.5.6.1197 · DOI
  32. 10.1104/pp.111.182253 · DOI
  33. 10.1093/genetics/138.4.1239 · DOI
  34. 10.1104/pp.123.2.563 · DOI
  35. 10.1105/tpc.111.089029 · DOI
  36. 10.1111/tpj.13032 · DOI
  37. 2022, Local conjugation of auxin by the GH3 amido synthetases is required for normal development of roots and flowers in Arabidopsis, Biochem. Biophys. Res. Commun., 589, 16, 10.1016/j.bbrc.2021.11.109 · DOI
  38. 10.1007/s10822-011-9439-8 · DOI
  39. 2017, Preference of Arabidopsis thaliana GH3.5 acyl amido synthetase for growth versus defense hormone acyl substrates is dictated by concentration of amino acid substrate aspartate, Phytochemistry, 143, 19, 10.1016/j.phytochem.2017.07.001 · DOI
  40. 10.1074/jbc.M806662200 · DOI
  41. 10.1126/science.1221863 · DOI
  42. 10.1074/jbc.M110.146431 · DOI
  43. 10.1104/pp.15.01360 · DOI
  44. 2019, OsIAGT1 is a glucosyltransferase gene involved in the glucose conjugation of auxins in rice, Rice, 12, 92, 10.1186/s12284-019-0357-z · DOI
  45. 10.1016/j.tplants.2019.05.006 · DOI
  46. 10.1007/BF00410211 · DOI
  47. 2022, The GH3 amidosynthetases family and their role in metabolic crosstalk modulation of plant signaling compounds, Phytochemistry, 194, 113039, 10.1016/j.phytochem.2021.113039 · DOI
  48. R. Casanova - Sáez , et al., Inactivation of the entire Arabidopsis group II GH3s confers tolerance to salinity and water deficit. New Phytol. (2022) https:/doi.org/10.1111/nph.18114. · DOI
  49. 10.1046/j.1365-313x.2001.00957.x · DOI
  50. 10.1046/j.1365-313X.2003.01973.x · DOI
  51. 10.1074/jbc.M610524200 · DOI
  52. 10.1104/pp.113.217174 · DOI
  53. 2016, Local auxin metabolism regulates environment-induced hypocotyl elongation, Nat. Plants, 2, 1
  54. 10.1016/j.cub.2019.02.022 · DOI

Metadata

Authors
Xie, Y., Zhu, Y., Wang, N., Luo, M., Ohta, T., Guo, R., Yu, Z., Aizezi, Y., Zhang, L., Yan, Y., Zhang, Y., Bao, H., Wang, Y., Zhu, Z., Huang, A., Zhao, Y., Asami, T., Huang, H., Guo, H., & Jiang, K.
Affiliations
  • Southern University of Science and Technology (CN)
  • University of California San Diego (US)
  • The University of Tokyo (JP)
  • Nanjing Normal University (CN)
Corresponding author
  • Huang, Hongda
  • Guo, Hongwei
  • Jiang, Kai
ORCID
Venue
Proceedings of the National Academy of Sciences
Publisher
openRxiv
Published
2022-05-30
Volume
119
Issue
49
Pages
e2209256119-e2209256119
ISSN
0027-8424 (ISSN-L)
DOI
10.1101/2022.05.29.493926
PMC
PMC9894192
OpenAlex
W4310493719
Cited by
29
References
1
Citations by year
2026: 5 · 2025: 13 · 2024: 6 · 2023: 4 · 2022: 1
FWCI
3.30
Type
journal article
Language
en
Open access
Licence
cc-by-nc-nd
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion, cc-by-nc-nd)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
  • PubMed Central (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • eScholarship (California Digital Library) (submittedVersion, cc-by-nc-nd)
Funders
  • National Natural Science Foundation of China (21907049, KQTD20190929173906742, KYTDPT20181011104005, 2019KSYS006, 2016ZT06S172, 31911540070)
  • China Postdoctoral Science Foundation (2020M672406)
  • Southern University of Science and Technology
  • Japan Society for the Promotion of Science (18H05266)
  • National Key Research and Development Program of China (2019YFA0903904)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Parasitism and Resistance
Keywords
auxin conjugation, indole-3-acetic acid, plant growth regulators, chemical genetic screening, Arabidopsis thaliana, enzyme inhibition, tomato, rice
MeSH terms
Genetic Testing, Indoleacetic Acids, Ligases, Plant Growth Regulators, Arabidopsis, Chemical Phenomena

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