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Cited by 741Plant Molecular Biology ResearchLight effects on plantsPlant nutrient uptake and metabolism

A novel sensor to map auxin response and distribution at high spatio-temporal resolution

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Brunoud, Géraldine, Wells, Darren M., Oliva, Marina, Larrieu, Antoine, Mirabet, Vincent, Burrow, Amy H., Beeckman, Tom, Kepinski, Stefan, Traas, Jan, Bennett, Malcolm J., Vernoux, Teva·2012·Nature
DOI 10.1038/nature10791
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Abstract

Auxin is a key plant morphogenetic signal but tools to analyse dynamically its distribution and signalling during development are still limited. Auxin perception directly triggers the degradation of Aux/IAA repressor proteins. Here we describe a novel Aux/IAA-based auxin signalling sensor termed DII-VENUS that was engineered in the model plant Arabidopsis thaliana. The VENUS fast maturing form of yellow fluorescent protein was fused in-frame to the Aux/IAA auxin-interaction domain (termed domain II; DII) and expressed under a constitutive promoter. We initially show that DII-VENUS abundance is dependent on auxin, its TIR1/AFBs co-receptors and proteasome activities. Next, we demonstrate that DII-VENUS provides a map of relative auxin distribution at cellular resolution in different tissues. DII-VENUS is also rapidly degraded in response to auxin and we used it to visualize dynamic changes in cellular auxin distribution successfully during two developmental responses, the root gravitropic response and lateral organ production at the shoot apex. Our results illustrate the value of developing response input sensors such as DII-VENUS to provide high-resolution spatio-temporal information about hormone distribution and response during plant growth and development.

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  • The adaxial boundary of leaf primordia, from which axillary meristems develop, is characterized by low auxin levels.

    “However, the adaxial boundary of leaf primordia, from which AMs develop, is characterized by low auxin levels, as suggested by the local distribution and polarization of the main auxin efflux carrier PIN1 (Figure 4) and the patterns of auxin response, monitored by the auxin sensors DR5:GFP and DII:VENUS (Figure 5) (Vernoux et al., 2011;Brunoud et al., 2012).”

    From Auxin Depletion from the Leaf Axil Conditions Competence for Axillary Meristem Formation in <i>Arabidopsis</i> and Tomato (2014) · cites this paper as Brunoud et al., 2012

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Authors
Brunoud, G., Wells, D. M., Oliva, M., Larrieu, A., Mirabet, V., Burrow, A. H., Beeckman, T., Kepinski, S., Traas, J., Bennett, M. J., & Vernoux, T.
Affiliations
  • Lyon 1 Université (FR)
  • University of Nottingham (GB)
  • University of Leeds (GB)
  • Ghent University (BE)
Corresponding author
Vernoux, Teva
ORCID
Venue
Nature
Publisher
Springer Science and Business Media LLC
Published
2012-01-15
Volume
482
Issue
7383
Pages
103-106
ISSN
  • 0028-0836 (ISSN-L)
  • 1476-4687
DOI
10.1038/nature10791
OpenAlex
W2014207396
Cited by
741
References
2
Citations by year
2026: 21 · 2025: 37 · 2024: 30 · 2023: 44 · 2022: 47 · 2021: 54 · 2020: 55 · 2019: 63 · 2018: 65 · 2017: 65 · 2016: 57 · 2015: 57 · 2014: 59 · 2013: 52 · 2012: 34
FWCI
73.29
Type
journal article
Language
en
Open access
Licence
Locations
  • Nature (publishedVersion)
  • PubMed (publishedVersion)
  • UWA Profiles and Research Repository (UWA) (submittedVersion)
  • HAL (submittedVersion)
  • RePEc: Research Papers in Economics (submittedVersion)
  • Ghent University Academic Bibliography (Ghent University) (submittedVersion)
  • Repository@Nottingham (University of Nottingham) (submittedVersion, cc-by)
  • Repository@Nottingham (University of Nottingham) (submittedVersion)
Funders
  • Agence Nationale de la Recherche (ANR-07-JCJC-0115)
  • Biotechnology and Biological Sciences Research Council (BB/F007418/1, BB/J009717/1, BB/F013981/1, REI20541, BB/D019613/1)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Light effects on plants
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant nutrient uptake and metabolism
Keywords
auxin, auxin distribution, auxin signaling, Aux/IAA repressors, proteasomal degradation, shoot apex, plant development, fluorescent protein fusion, Arabidopsis thaliana
MeSH terms
Bacterial Proteins, Indoleacetic Acids, Luminescent Proteins, Organ Specificity, Time Factors, Arabidopsis, Protein Structure, Tertiary, Gene Expression Regulation, Plant, Plant Shoots, Gravitropism, Plants, Genetically Modified, Proteasome Endopeptidase Complex

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