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PreprintCited by 46Toxoplasma gondii Research StudiesMicrotubule and mitosis dynamicsCoccidia and coccidiosis research

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Conoid extrusion serves as gatekeeper for entry of glideosome components into the pellicular space to control motility and invasion in Apicomplexa

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Dos Santos Pacheco, Nicolas, Brusini, Lorenzo, Haase, Romuald, Tosetti, Nicolò, Maco, Bohumil, Brochet, Mathieu, Vadas, Oscar, Soldati-Favre, Dominique·2022·Nature Microbiology
DOI 10.1101/2022.06.06.494926

Preprint — published as 10.1038/s41564-022-01212-x · Paper page

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Abstract

SUMMARY: Members of the Apicomplexa are defined by apical cytoskeletal structures and secretory or-ganelles, tailored for motility and invasion. Gliding is powered by actomyosin-dependent rearward translocation of apically secreted transmembrane adhesins. In Toxoplasma gondii , the conoid, composed of a cone of spiraling tubulin fibers and apposed preconoidal rings (PCRs), is an enigmatic, dynamic organelle of undefined function. Here we mapped five new components of the PCRs and deduce that the structure serves as a pivotal hub for actin polymerization and glideosome assembly. F-actin produced by Formin1 on the PCRs is used by Myosin H to generate the force for conoid extrusion. A set of B-box-type zinc finger domain containing proteins conserved in Apicomplexa is indispensable for PCRs formation, conoid extrusion and motility in Toxoplasma and Plasmodium . Conoid dynamics directs the flux of F-actin to the pellicular space, acting as dynamic gatekeeper to tightly control parasite motility during invasion and egress.

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Metadata

Authors
Dos Santos Pacheco, N., Brusini, L., Haase, R., Tosetti, N., Maco, B., Brochet, M., Vadas, O., & Soldati-Favre, D.
Affiliations
University of Geneva (CH)
Corresponding author
Vadas, Oscar
ORCID
Venue
Nature Microbiology
Publisher
openRxiv
Published
2022-06-06
Volume
7
Issue
11
Pages
1777-1790
ISSN
2058-5276 (ISSN-L)
DOI
10.1101/2022.06.06.494926
OpenAlex
W4295925642
Cited by
46
References
1
Citations by year
2026: 9 · 2025: 8 · 2024: 18 · 2023: 8 · 2022: 3
FWCI
7.35
Type
journal article
Language
en
Open access
Licence
cc-by-nc
Locations
  • Nature Microbiology (publishedVersion)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc)
  • PubMed (publishedVersion)
  • Archive ouverte UNIGE (University of Geneva) (publishedVersion)
  • Archive ouverte UNIGE (University of Geneva) (submittedVersion, cc-by)
Funders
  • Novartis Foundation (19C189)
  • European Commission (HORIZON2020, 695596)
  • École Polytechnique Fédérale de Lausanne
  • Université de Genève
Topics
  • Life Sciences › Immunology and Microbiology › Parasitology › Toxoplasma gondii Research Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Coccidia and coccidiosis research
Keywords
Apicomplexa, Toxoplasma gondii, parasite invasion, egress, ultrastructure expansion microscopy
MeSH terms
Actins, Cytoskeleton, Humans, Toxoplasma, Tubulin, Protozoan Proteins, Apicomplexa

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