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Cited by 218Toxoplasma gondii Research StudiesCoccidia and coccidiosis researchMalaria Research and Control

A Novel Family of Toxoplasma IMC Proteins Displays a Hierarchical Organization and Functions in Coordinating Parasite Division

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Beck, Josh R., Rodriguez-Fernandez, Imilce A., Cruz de Leon, Jessica, Huynh, My-Hang, Carruthers, Vern B., Morrissette, Naomi S., Bradley, Peter J.·2010·PLoS Pathogens
DOI 10.1371/journal.ppat.1001094PMC PMC2936552
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Abstract

Apicomplexans employ a peripheral membrane system called the inner membrane complex (IMC) for critical processes such as host cell invasion and daughter cell formation. We have identified a family of proteins that define novel sub-compartments of the Toxoplasma gondii IMC. These IMC Sub-compartment Proteins, ISP1, 2 and 3, are conserved throughout the Apicomplexa, but do not appear to be present outside the phylum. ISP1 localizes to the apical cap portion of the IMC, while ISP2 localizes to a central IMC region and ISP3 localizes to a central plus basal region of the complex. Targeting of all three ISPs is dependent upon N-terminal residues predicted for coordinated myristoylation and palmitoylation. Surprisingly, we show that disruption of ISP1 results in a dramatic relocalization of ISP2 and ISP3 to the apical cap. Although the N-terminal region of ISP1 is necessary and sufficient for apical cap targeting, exclusion of other family members requires the remaining C-terminal region of the protein. This gate-keeping function of ISP1 reveals an unprecedented mechanism of interactive and hierarchical targeting of proteins to establish these unique sub-compartments in the Toxoplasma IMC. Finally, we show that loss of ISP2 results in severe defects in daughter cell formation during endodyogeny, indicating a role for the ISP proteins in coordinating this unique process of Toxoplasma replication.

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  • The inner membrane complex of T. gondii has unique and critical roles in parasite replication, motility and invasion.

    “The inner membrane complex (IMC) of Toxoplasma gondii as a peripheral membrane system has unique and critical roles in parasite replication, motility and invasion.”

    From Virus-Like Nanoparticle Vaccine Confers Protection against Toxoplasma gondii (2016) · cites this paper as [8]

  • ISP1, ISP2, and ISP3 localize to distinct sub-compartments of the IMC in T. gondii.

    “Three proteins, IMC sub-compartment protein (ISP) 1, ISP2, and ISP3, were initially identified and shown to localize to distinct sub-compartments of the IMC in T. gondii [8].”

    From Virus-Like Nanoparticle Vaccine Confers Protection against Toxoplasma gondii (2016) · cites this paper as [8]

  • Disruption of TgISP2 markedly reduces parasite fitness and produces a severe defect in endodyogeny in T. gondii.

    “Disruption of TgISP2 markedly reduces parasite fitness and produces a severe defect in endodyogeny, the form of internal cell budding in which two daughter cells are formed within the intact mother parasite [8].”

    From Virus-Like Nanoparticle Vaccine Confers Protection against Toxoplasma gondii (2016) · cites this paper as [8]

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  1. E Porchet, 1977, [Freeze fracture study of <italic>Toxoplasma</italic> and <italic>Sarcocystis</italic> infective stages (author&apos;s transl)]., Z Parasitenkd, 54, 101

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Authors
Beck, J. R., Rodriguez-Fernandez, I. A., Cruz de Leon, J., Huynh, M.-H., Carruthers, V. B., Morrissette, N. S., & Bradley, P. J.
Affiliations
  • University of California, Los Angeles (US)
  • University of Michigan (US)
  • University of California, Irvine (US)
Corresponding author
Bradley, Peter J.
ORCID
Venue
PLoS Pathogens
Publisher
Public Library of Science (PLoS)
Published
2010-09-09
Volume
6
Issue
9
Pages
e1001094
ISSN
  • 1553-7366 (ISSN-L)
  • 1553-7374
DOI
10.1371/journal.ppat.1001094
PMC
PMC2936552
OpenAlex
W1996756350
Cited by
217
References
61
Citations by year
2026: 4 · 2025: 17 · 2024: 15 · 2023: 13 · 2022: 10 · 2021: 22 · 2020: 15 · 2019: 20 · 2018: 8 · 2017: 14 · 2016: 17 · 2015: 15 · 2014: 12 · 2013: 12 · 2012: 13
FWCI
8.62
Type
journal article
Language
en
Open access
Licence
Locations
  • PLoS Pathogens (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • eScholarship (California Digital Library) (submittedVersion, cc-by)
  • Figshare (submittedVersion, cc-by)
Funders
  • National Institutes of Health (4r01ai064616-10, NIH-R01)
  • U.S. Public Health Service (R01 #A121423)
  • National Institute of Allergy and Infectious Diseases (R01 #AI064616, T32AI07323, R01AI067981, R01AI046675, T32AI007323)
Topics
  • Life Sciences › Immunology and Microbiology › Parasitology › Toxoplasma gondii Research Studies
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Coccidia and coccidiosis research
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Malaria Research and Control
Keywords
Toxoplasma gondii, inner membrane complex, apical cap, protein subcellular targeting, myristoylation, palmitoylation, endodyogeny, cell division, Apicomplexa, parasite replication
MeSH terms
Amino Acid Sequence, Animals, Cell Division, Cell Membrane, Cells, Cultured, Fibroblasts, Humans, Immunoglobulin G, Immunization, Male, Membrane Proteins, Mice, Inbred BALB C, Molecular Sequence Data, RNA, Messenger, Toxoplasma, Toxoplasmosis, Blotting, Western, Protozoan Proteins, Mutagenesis, Site-Directed, Sequence Homology, Amino Acid, Reverse Transcriptase Polymerase Chain Reaction, Mice, Foreskin

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