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PreprintCited by 51Advanced Electron Microscopy Techniques and ApplicationsEnzyme Structure and FunctionProtein Structure and Dynamics

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Structure of Anabaena flos-aquae gas vesicles revealed by cryo-ET

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Dutka, Przemysław, Metskas, Lauren Ann, Hurt, Robert C., Salahshoor, Hossein, Wang, Ting-Yu, Malounda, Dina, Lu, George, Chou, Tsui-Fen, Shapiro, Mikhail G., Jensen, Grant J.·2022·Structure
DOI 10.1101/2022.06.21.496981PMC PMC10185304

Preprint — published as 10.1016/j.str.2023.03.011 · Paper page

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Abstract

SUMMARY: Gas vesicles (GVs) are gas-filled protein nanostructures employed by several species of bacteria and archaea as flotation devices to enable access to optimal light and nutrients. The unique physical properties of GVs have led to their use as genetically-encodable contrast agents for ultrasound and MRI. Currently, however, the structure and assembly mechanism of GVs remain unknown. Here we employ cryo-electron tomography to reveal how the GV shell is formed by a helical filament of highly conserved GvpA subunits. This filament changes polarity at the center of the GV cylinder—a site that may act as an elongation center. High-resolution subtomogram averaging reveals a corrugated pattern of the shell arising from polymerization of GvpA into a β-sheet. The accessory protein GvpC forms a helical cage around the GvpA shell, providing structural reinforcement. Together, our results help explain the remarkable mechanical properties of GVs and their ability to adopt different diameters and shapes.

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Metadata

Authors
Dutka, P., Metskas, L. A., Hurt, R. C., Salahshoor, H., Wang, T.-Y., Malounda, D., Lu, G., Chou, T.-F., Shapiro, M. G., & Jensen, G. J.
Affiliations
  • California Institute of Technology (US)
  • Brigham Young University (US)
Corresponding author
  • Shapiro, Mikhail G.
  • Jensen, Grant J.
ORCID
Venue
Structure
Publisher
openRxiv
Published
2022-06-21
Volume
31
Issue
5
Pages
518-528.e6
ISSN
0969-2126 (ISSN-L)
DOI
10.1101/2022.06.21.496981
PMC
PMC10185304
OpenAlex
W4363675958
Cited by
51
References
1
Citations by year
2026: 10 · 2025: 15 · 2024: 17 · 2023: 8 · 2022: 1
FWCI
4.40
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Structure (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion, cc-by)
  • Zenodo (CERN European Organization for Nuclear Research) (submittedVersion, cc-by)
Funders
  • David and Lucile Packard Foundation
  • Howard Hughes Medical Institute
  • California Institute of Technology
  • Heritage Medical Research Institute
  • Chan Zuckerberg Initiative
  • Royal Society
  • National Institutes of Health (1s10od020013-01)
  • Directorate for Biological Sciences
  • Goddard Space Flight Center
  • University of California, San Francisco
  • Center for Environmental Microbial Interactions, California Institute of Technology
  • National Institute of Allergy and Infectious Diseases (R01AI127401)
  • National Institute of Biomedical Imaging and Bioengineering (R01EB018975)
  • Beckman Institute for Advanced Science and Technology, University of Illinois, Urbana-Champaign
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Structural Biology › Advanced Electron Microscopy Techniques and Applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Structural Biology › Enzyme Structure and Function
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
Keywords
gas vesicles, Anabaena flos-aquae, helical filaments, assembly mechanism, subtomogram averaging, cryo-electron tomography
MeSH terms
Archaea, Bacterial Proteins, Anabaena, Dolichospermum flos-aquae

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