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PreprintCited by 15DNA Repair MechanismsFungal and yeast genetics researchMicrotubule and mitosis dynamics

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Mer3 helicase protects early crossover intermediates from STR complex disassembly during meiosis

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Altmannova, Veronika, Firlej, Magdalena, Müller, Franziska, Janning, Petra, Rauleder, Rahel, Rousova, Dorota, Schäffler, Andreas, Weir, John R.·2022·Nucleic Acids Research
DOI 10.1101/2022.06.27.497840PMC PMC10201424

Preprint — published as 10.1093/nar/gkad175 · Paper page

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Abstract

During meiosis I it is necessary that homologous chromosomes are linked to one another so that they can be faithfully separated. S. cerevisiae Mer3 (HFM1 in mammals) is a SF2 helicase and member of the ZMM group of proteins, that facilitates the formation of class I crossovers during meiosis. Here we describe the structural organisation of Mer3 and, using AlphaFold modelling and XL-MS, we further characterise the previously described interaction with Mlh1-Mlh2. We find that Mer3 also forms a previously undescribed complex with the recombination regulating factors Top3 and Rmi1 and that this interaction is competitive with Sgs1 BLM helicase in a phospho-dependent manner. Using in vitro reconstituted D-loop assays we show that Mer3 inhibits the anti-recombination activity of Sgs1/Top3/Rmi1 (STR) complex. Thus we provide a mechanism whereby Mer3 downregulates the anti-crossover activity of the STR complex, hence promoting the formation of crossovers during meiosis I.

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Research works printed but not linked: 57

  1. 2021, InteBac: An integrated bacterial and baculovirus expression vector suite, Protein Sci, 30, 108, 10.1002/pro.3957 · DOI
  2. 2010, The roles of the Saccharomyces cerevisiae RecQ helicase SGS1 in meiotic genome surveillance, PLoS One, 5, e15380, 10.1371/journal.pone.0015380 · DOI
  3. 10.1093/nar/gkl258 · DOI
  4. 10.1016/S0092-8674(00)80717-5 · DOI
  5. 2000, Interaction between Yeast Sgs1 Helicase and DNA Topoisomerase III *, J. Biol. Chem, 275, 26898, 10.1016/S0021-9258(19)61459-6 · DOI
  6. 10.1146/annurev-genet-102209-163602 · DOI
  7. 10.1016/j.tibs.2011.10.005 · DOI
  8. 2014, The dissolution of double Holliday junctions. Cold Spring Harb. Perspect, Biol, 6, a016477
  9. 2014, Structural and mechanistic insight into Holliday-junction dissolution by topoisomerase III?, and RMI1. Nat. Struct. Mol. Biol, 21, 261, 10.1038/nsmb.2775 · DOI
  10. 10.1016/S0092-8674(04)00292-2 · DOI
  11. 10.1021/bi0018640 · DOI
  12. 10.1038/nsmb.1919 · DOI
  13. 10.1016/j.molcel.2012.06.032 · DOI
  14. 10.1038/sj.emboj.7600684 · DOI
  15. 10.1111/j.1365-313X.2005.02461.x · DOI
  16. 10.1016/j.molcel.2012.02.020 · DOI
  17. 10.1101/gad.308510.117 · DOI
  18. 10.7554/eLife.21900 · DOI
  19. Evans, R. , O’Neill, M. , Pritzel, A. , Antropova, N. , Senior, A. , Green, T. , Žk, A. , Bates, R. , Blackwell, S. , Yim, J. , et al. (2021). Protein complex prediction with AlphaFold-Multimer. · DOI · Paper page
  20. 2020, Phosphorylation of the RecQ Helicase Sgs1/BLM Controls Its DNA Unwinding Activity during Meiosis and Mitosis. Dev, Cell, 53, 706
  21. 10.1093/nar/gkv463 · DOI · Paper page
  22. 10.1371/journal.pgen.1003383 · DOI
  23. 2020, Using Dali for Protein Structure Comparison. Methods Mol, Biol, 2112, 29
  24. 2014, A non-sister act: Recombination template choice during meiosis. Exp, Cell Res, 329, 53, 10.1016/j.yexcr.2014.08.024 · DOI
  25. 10.1016/S0092-8674(01)00430-5 · DOI
  26. 10.1002/yea.1142 · DOI
  27. 10.1371/journal.pgen.0020155 · DOI
  28. 2000, Association of the Bloom syndrome protein with topoisomerase IIIalpha in somatic and meiotic cells, Cancer Res, 60, 1162
  29. 2019, Competing interaction partners modulate the activity of Sgs1 helicase during DNA end resection, EMBO J, 38, e101516, 10.15252/embj.2019101516 · DOI
  30. 10.1002/(SICI)1097-0061(199907)15:10B<963::AID-YEA399>3.0.CO;2-W · DOI
  31. Lagerwaard, I.M. , Albanese, P. , Jankevics, A. , and Scheltema, R.A. (2022). Xlink Mapping and AnalySis (XMAS) -Smooth Integrative Modeling in ChimeraX. · DOI
  32. 2014, Mechanism and regulation of meiotic recombination initiation. Cold Spring Harb. Perspect, Biol, 7, a016634
  33. 10.1073/pnas.1104421108 · DOI
  34. 10.1007/s10577-007-1150-1 · DOI
  35. 10.1016/S0092-8674(04)00294-6 · DOI
  36. 10.1016/j.cub.2005.02.056 · DOI
  37. 10.1128/MCB.25.11.4476-4487.2005 · DOI
  38. 10.1021/ac101241t · DOI
  39. 10.1128/MCB.22.10.3281-3291.2002 · DOI
  40. 10.1074/jbc.M104003200 · DOI
  41. 10.1016/j.cell.2007.05.035 · DOI
  42. 10.1021/acs.analchem.8b02593 · DOI
  43. 2009, Common design principles in the spliceosomal RNA helicase Brr2 and in the Hel308 DNA helicase. Mol, Cell, 35, 454
  44. 2000, Proteins of the endoplasmic-reticulum-associated degradation pathway: domain detection and function prediction, Biochem. J 351 Pt, 2, 527
  45. 10.1007/s00412-019-00714-8 · DOI
  46. 10.1074/jbc.M113.533810 · DOI
  47. 2020, PyXlinkViewer: A flexible tool for visualization of protein chemical crosslinking data within the PyMOL molecular graphics system, Protein Sci, 29, 1851, 10.1002/pro.3902 · DOI
  48. 10.1038/ng.83 · DOI
  49. 10.1016/j.cell.2010.02.041 · DOI
  50. Tang, S. , Wu, M.K.Y. , Zhang, R. , and Hunter, N. (2015). Pervasive and Essential Roles of the Top3-Rmi1 Decatenase Orchestrate Recombination and Facilitate Chromosome Segregation in Meiosis. Mol. Cell 57. https://doi.org/10.1016/j.molcel.2015.01.021. · DOI
  51. 2021, The Pif1 helicase is actively inhibited during meiotic recombination which restrains gene conversion tract length, Nucleic Acids Res, 49, 4522, 10.1093/nar/gkab232 · DOI
  52. 10.1016/S0006-291X(02)02034-X · DOI
  53. 10.1056/NEJMc1310150 · DOI
  54. 10.1242/jcs.049080 · DOI
  55. 2016, biGBac enables rapid gene assembly for the expression of large multisubunit protein complexes, Proc. Natl. Acad. Sci. U. S. A, 113, E2564 9
  56. 10.1016/j.molcel.2019.06.022 · DOI
  57. 2021, Mechanism and Control of Meiotic DNA Double-Strand Break Formation in S. cerevisiae, Front Cell Dev Biol, 9, 642737, 10.3389/fcell.2021.642737 · DOI

Metadata

Authors
Altmannova, V., Firlej, M., Müller, F., Janning, P., Rauleder, R., Rousova, D., Schäffler, A., & Weir, J. R.
Affiliations
  • Max Planck Society (DE)
  • Max Planck Institute of Molecular Physiology (DE)
Corresponding author
Weir, John R.
ORCID
Venue
Nucleic Acids Research
Publisher
openRxiv
Published
2022-06-27
Volume
51
Issue
9
Pages
4363-4384
ISSN
0305-1048 (ISSN-L)
DOI
10.1101/2022.06.27.497840
PMC
PMC10201424
OpenAlex
W4328049435
Cited by
15
References
1
Citations by year
2026: 5 · 2025: 5 · 2024: 2 · 2023: 3
FWCI
1.34
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Nucleic Acids Research (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
  • Wellcome Trust
  • Deutsche Forschungsgemeinschaft
  • Max-Planck-Gesellschaft
  • Masarykova Univerzita
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
Keywords
meiotic recombination, crossing over, Sgs1 helicase, BLM helicase, DMC1, protein-protein interaction, Saccharomyces cerevisiae
MeSH terms
MutL Proteins, MutL Protein Homolog 1, Binding, Competitive, Crossing Over, Genetic, DNA Helicases, DNA-Binding Proteins, Meiosis, Protein Binding, Saccharomyces cerevisiae, Protein Folding, Cell Cycle Proteins, Saccharomyces cerevisiae Proteins, RecQ Helicases, Homologous Recombination

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