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The SSU Processome Component Utp25p is a Pseudohelicase
Open accessPreprint — published as 10.17912/micropub.biology.000606 · Paper page
Abstract
RNA helicases are involved in nearly all aspects of RNA metabolism and factor prominently in ribosome assembly. The SSU processome includes 10 helicases and many helicase-cofactors. Together, they mediate the structural rearrangements that occur during ribosomal SSU assembly. During the identification of the SSU processome component Utp25, it was noticed that the protein displays some sequence similarity to DEAD-box RNA helicases and is essential for growth. Interestingly, mutational ablation showed that Utp25’s DEAD-box motifs are dispensable. Here, we use AlphaFold to show that Utp25 retains considerable structural similarity to DEAD-box helicases and is the first fully validated pseudohelicase.
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Metadata
- Authors
- Helwer, R., & Charette, J. M.
- Affiliations
- CancerCare Manitoba (CA)
- Corresponding author
- Charette, J. Michael
- Venue
- bioRxiv (Cold Spring Harbor Laboratory)
- Publisher
- openRxiv
- Published
- 2022-07-09
- OpenAlex
- W4284993352
- Cited by
- 1
- References
- 37
- Citations by year
- 2024: 1
- Type
- posted content
- Language
- en
- Open access
- green
- doi.org
- Licence
- cc-by-nc-nd
- Locations
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
- Funders
- Natural Sciences and Engineering Research Council of Canada (RGPIN-2016, rgpin-2016-06599)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
- Keywords
- DEAD-box RNA helicase, RNA helicase, ribosome assembly, protein structure prediction, essential proteins
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