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PreprintCited by 4Plant nutrient uptake and metabolismNitrogen and Sulfur Effects on BrassicaAmino Acid Enzymes and Metabolism

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A new gene encoding a cytosolic glutamine synthetase in pine is linked to developing tissues

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Valderrama-Martín, José Miguel, Ortigosa, Francisco, Aledo, Juan Carlos, Ávila, Concepción, Cánovas, Francisco M., Cañas, Rafael A.·2022·The Plant Journal
DOI 10.1101/2022.10.27.514046

Preprint — published as 10.1111/tpj.16113 · Paper page

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Abstract

SUMMARY: The enzyme glutamine synthetase (EC 6.3.1.2) is mainly responsible for the incorporation of inorganic nitrogen into organic molecules in plants. In the present work, a new pine GS1 ( PpGS1b.2 ) gene was identified, showing a high sequence identity with the GS1b.1 gene previously characterized in conifers. Phylogenetic analysis revealed that the presence of PpGS1b.2 is restricted to the genera Pinus and Picea and is not found in other conifers. Gene expression data suggest a putative role of PpGS1b.2 in plant development, similar to other GS1b genes from angiosperms, suggesting evolutionary convergence. The characterization of GS1b.1 and GS1b.2 at the structural, physicochemical, and kinetic levels has shown differences even though they have high sequence homology. Alterations in the kinetic characteristics produced by the site-directed mutagenesis approach carried out in this work strongly suggest an implication of amino acids at positions 264 and 267 in the active center of pine GS1b.1 and GS1b.2. Therefore, the amino acid differences between GS1b.1 and GS1b.2 would support the functioning of both enzymes to meet distinct plant needs.

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Research works printed but not linked: 80

  1. 10.1093/nar/gky379 · DOI · Paper page
  2. 2021, ptm: an R package for the study of methionine sulfoxidation and other post-translational modifications, Bioinformatics, 37, 3979, 10.1093/bioinformatics/btab348 · DOI
  3. 10.1006/jmbi.1990.9999 · DOI · Paper page
  4. 10.1046/j.1365-313x.2001.00938.x · DOI
  5. 2022, Identification of metabolic pathways differentially regulated in somatic and zygotic embryos of maritime pine, Frontiers in Plant Science, 13, 877960, 10.3389/fpls.2022.877960 · DOI
  6. Bao, A. , Zhao, Z. , Ding, G. , Shi, L. , Xu, F. & Cai, H. (2014) Accumulated expression level of cytosolic glutamine synthetase 1 gene (OsGs1;1 or OsGS1;2) alter plant development and the carbon-nitrogen metabolic status in rice. PLoS ONE, 9, https://doi.org/10.1371/journal.pone.0095581 · DOI
  7. 10.1111/j.1469-8137.2009.02823.x · DOI
  8. 1987, Inhibition of photosynthesis in barley with decreased levels of chloroplastic glutamine synthetase activity, Journal of Experimental Botany, 196, 1799
  9. 10.1093/bioinformatics/btu170 · DOI · Paper page
  10. 10.1016/0003-2697(76)90527-3 · DOI · Paper page
  11. 2012, Novel insights into regulation of asparagine synthetase in conifers, Frontiers in Plant Science, 3, 100
  12. 1991, Accumulation of glutamine synthetase during early development of maritime pine seedlings (Pinus pinaster), Planta, 185, 372, 10.1007/BF00201059 · DOI
  13. 10.1016/0014-5793(96)00886-1 · DOI
  14. 1999, Expression analysis of a cytosolic glutamine synthetase gene in cotyledons of Scots pine seedlings: developmental, light regulation and spatial distribution of specific transcripts, Plant Molecular Biology, 404, 623
  15. 10.1093/treephys/tpt113 · DOI
  16. 10.1093/jxb/erv118 · DOI
  17. 2017, The gene expression landscape of pine seedling tissues, The Plant Journal, 91, 1064, 10.1111/tpj.13617 · DOI
  18. 2015, Redundancy and metabolic function of the glutamine synthetase gene family in poplar, BMC Plant Biology, 15, 20, 10.1186/s12870-014-0365-5 · DOI
  19. 10.1093/pcp/pcf094 · DOI
  20. 10.1016/j.pbi.2012.01.003 · DOI
  21. 10.1186/1472-6750-9-61 · DOI
  22. 10.1093/nar/gkh340 · DOI · Paper page
  23. 10.1093/jxb/erm121 · DOI
  24. 10.1093/jxb/ert335 · DOI
  25. 2022, Cytosolic glutamine synthetase GS1;3 is involved in rice grain ripening and germination, Frontiers in Plant Science, 13, 835835, 10.3389/fpls.2022.835835 · DOI
  26. 10.1093/pcp/pct046 · DOI
  27. 2019, Cisgenic overexpression of cytosolic glutamine synthetase improves nitrogen utilization efficiency in barley and prevents grain protein decline under elevated CO2, Plant Biotechnology Journal, 17, 1209, 10.1111/pbi.13046 · DOI
  28. 10.1016/j.ab.2003.12.024 · DOI
  29. 10.1111/j.1399-3054.2004.00353.x · DOI
  30. 10.1007/s00425-003-1185-2 · DOI
  31. 10.1093/pcp/pct006 · DOI
  32. 10.1038/nbt.1883 · DOI
  33. 10.1093/jxb/eru411 · DOI
  34. 2016, Selection and testing of reference genes for accurate RT-qPCR in adult needles and seedlings of maritime pine, Tree Genetic & Genomes, 12, 60, 10.1007/s11295-016-1018-7 · DOI
  35. 10.1111/j.1744-7348.2001.tb00087.x · DOI
  36. 2021, Excessive ammonium assimilation by plastidic glutamine synthetase causes ammonium toxicity in Arabidopsis thaliana, Nature Communications, 12, 4944, 10.1038/s41467-021-25238-7 · DOI
  37. Heldt, H. & Piechulla, B. (2011) Plant Biochemestry: fourth edition. San Diego: Academic Press, Elsevier.
  38. Hirel, B. & Krapp, A. (2021) Nitrogen utilization in plants I biological and agronomic importance. In: Jez, J. (Ed.) Encyclopedia of biological chemistry III, Third edition. Amsterdam: Elsevier, pp. 127–140. · DOI
  39. 10.1093/pcp/pch190 · DOI
  40. 10.1074/jbc.M313710200 · DOI
  41. 10.1093/pcp/pci238 · DOI
  42. James, D. , Borphukan, B. , Fartyal, D. , Achary, V.M.M. & Reddy, M.K. (2018) Transgenic manipulation of glutamine synthetase: A target with untapped potential in various aspects of crop improvement. In: Gosal SS , Wani SH , editors. Biotechnology of Crop Improvement. Cham: Springer International Publishing AG, pp 367–416. · DOI
  43. 2019, Roles of cytosolic glutamine synthetase in Arabidopsis development and stress responses, Plant and Cell Physiology, 60, 657, 10.1093/pcp/pcy235 · DOI
  44. 10.1093/bioinformatics/8.3.275 · DOI
  45. 10.1104/pp.114.251298 · DOI
  46. Lea, P.J. & Ireland, R.J. (1999) Nitrogen metabolism in higher Plants. In: Singh BK , editor. Plant Amino Acids. New York: Marcel Dekker Inc, pp. 1–47.
  47. 10.1016/B978-0-12-152810-2.50008-5 · DOI
  48. 10.1016/j.jsb.2006.06.003 · DOI
  49. López-Bucio, J.S. , de la Cruz, H.R. & Guevara-García, A.A. (2019) Glutamate sensing in plants. In: Ramakrishna A , Roshchina VV , editors. Neurotransmitters in plants: perspectives and applications. Raton: CRC Press, pp. 231–140. · DOI
  50. 10.1093/jxb/erq299 · DOI
  51. 10.1126/science.1201101 · DOI
  52. 10.1105/tpc.106.042689 · DOI
  53. 2022, ColabFold: making protein folding accessible to all, Nature Methods, 19, 679, 10.1038/s41592-022-01488-1 · DOI · Paper page
  54. 2022, Glutamine synthetase: an unlikely case of functional redundancy in Arabidopsis thaliana, Plant Biology, 24, 713, 10.1111/plb.13408 · DOI
  55. 2005, Expression patterns of two glutamine synthetase genes in zygotic and somatic pine embryos support specific roles in nitrogen metabolism during embryogenesis, New Phytologist, 169, 35
  56. 2020, Signaling role of glutamate in plants, Frontiers in Plant Science, 13, 1743
  57. 2022, Ammonium regulates the development of pine roots through hormonal crosstalk and differential expression of transcription factors in the apex, Plant, Cell and Environment, 45, 915, 10.1111/pce.14214 · DOI
  58. 10.1093/bioinformatics/btn227 · DOI
  59. 10.1074/jbc.271.47.29561 · DOI
  60. 1994, Loblolly pine seed dormancy. I. The relationship between protein synthesis and the loss of dormancy, Physiologia Plantarum, 90, 246, 10.1111/j.1399-3054.1994.tb00384.x · DOI
  61. 2014, Prediction of protein structure and interaction by GALAXY protein modeling programs, BioDesign, 2, 1
  62. Sterck, L. , de María, N. , Cañas, R.A. , de Miguel, M. , Perdiguero, P. , Raffin, A. et al. (2022) Maritime Pine Genomics in Focus. In: De La Torre, A.R. (eds) The Pine Genomes. Compendium of Plant Genomes. Springer, Cham, 67–123. · DOI
  63. 10.1093/jexbot/53.370.891 · DOI
  64. 10.1111/j.1365-313X.2005.02406.x · DOI
  65. 10.1093/MOLBEV/MSAB120 · DOI
  66. 2017, Source and sink mechanisms of nitrogen transport and use, New Phytologist, 217, 35
  67. 2014, Cytosolic glutamine synthetase: a target for improvement of crop nitrogen use efficiency?, Trends in Plant Science, 19, 656, 10.1016/j.tplants.2014.06.002 · DOI
  68. 2020, PlantRegMap: charting functional regulatory maps in plants, Nucleic Acids Research, 48, D1104
  69. 2015, Overexpression of a glutamine synthetase gene affects growth and development in sorghum, Transgenic Research, 24, 397, 10.1007/s11248-014-9852-6 · DOI
  70. 2022, A revised view on the evolution of glutamine synthetase isoenzymes in plants, The Plant Journal, 110, 946, 10.1111/tpj.15712 · DOI
  71. 10.1093/NAR/GKAB1061 · DOI · Paper page
  72. Villalobos, D.P. (2008) Aproximación genómica al estudio de la formación de la madera en los pinos. Universidad de Málaga, Málaga (Spain). Doctoral dissertation.
  73. 10.1104/pp.83.1.155 · DOI
  74. 1976, Catalytic mechanisms of glutamine synthetase enzymes. Studies with analogs of possible intermediates and transition states, Journal of Biological Chemistry, 251, 7530, 10.1016/S0021-9258(17)32883-1 · DOI
  75. 2021, Localization, gene expression, and functions of glutamine synthetase isoenzymes in wheat grain (Trticum aestivum L.), Frontiers in Plant Science, 12, 580405, 10.3389/fpls.2021.580405 · DOI
  76. 2018, CORNICHON sorting and regulation of GLR channels underlie pollen tube Ca2+ homeostasis, Plant Science, 360, 533
  77. 2009, Computational structural analysis and kinetic studies of a cytosolic glutamine synthetase from Camellia sinensis (L.) O. Kuntze, Protein Journal, 28, 428, 10.1007/s10930-009-9210-3 · DOI
  78. 10.1093/jxb/eru103 · DOI
  79. 2014, Expression, purification, and characterization of recombinant mangrove glutamine synthetase, Molecular Biology Reports, 41, 7575, 10.1007/s11033-014-3649-9 · DOI
  80. 2021, Regulation of cytosolic pH: the contributions of plant plasma membrane H+-ATPases and multiple transporters, International Journal of Molecular Sciences, 22, 12998, 10.3390/ijms222312998 · DOI

Metadata

Authors
Valderrama-Martín, J. M., Ortigosa, F., Aledo, J. C., Ávila, C., Cánovas, F. M., & Cañas, R. A.
Affiliations
Universidad de Málaga (ES)
Corresponding author
Cañas, Rafael A.
ORCID
Venue
The Plant Journal
Publisher
openRxiv
Published
2022-10-28
Volume
113
Issue
6
Pages
1330-1347
ISSN
0960-7412 (ISSN-L)
DOI
10.1101/2022.10.27.514046
OpenAlex
W4317568126
Cited by
4
References
1
Citations by year
2026: 1 · 2025: 1 · 2024: 1 · 2023: 1
FWCI
0.63
Type
journal article
Language
en
Open access
Licence
cc-by-nc
Locations
  • The Plant Journal (publishedVersion, cc-by-nc)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • Repositorio Institucional de la Universidad de Málaga (University of Málaga) (submittedVersion, cc-by-nc)
  • Repositorio Institucional de la Universidad de Málaga (University of Málaga) (submittedVersion, other-oa)
Funders
  • Universidad de Málaga (UMAJI11, B4‐2021‐01)
  • Ministerio de Economía y Competitividad (BIO2015-73512-JIN)
  • Ministerio de Ciencia e Innovación (PID2021-125040OB-I00, PAIDI 2020, FPU17)
  • Junta de Andalucía (PAIDI 2020, B4-2021, BIO-114)
  • Ministerio de Educación y Formación Profesional (FPU17/03517)
  • Agencia de Innovación y Desarrollo de Andalucía (P20_00036 PAIDI 2020/FEDER, UE, P20_00036 PAIDI 2020/FEDER, BIO‐114)
  • Secretaría de Estado de Investigación, Desarrollo e Innovación (BIO2015‐69285‐R, RTI2018‐094041‐B‐I00, BIO2015‐73512‐JIN MINECO/AEI/FEDER, BIO2015‐73512‐JIN MINECO/AEI/FEDER, UE)
  • Agencia Estatal de Investigación (PID2021-125040OB-I00, PID2021)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant nutrient uptake and metabolism
  • Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Nitrogen and Sulfur Effects on Brassica
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Amino Acid Enzymes and Metabolism
Keywords
glutamine synthetase, Pinus pinaster, paralogs, conifers, nitrogen assimilation, enzyme kinetics, substrate inhibition, cooperativity, thermal stability, pH optimum, active site residues, site-directed mutagenesis, phylogenetic analysis, gene expression, plant development, convergent evolution
MeSH terms
Glutamate-Ammonia Ligase, Phylogeny, Glutamic Acid, Pinus, Ammonium Compounds

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CoChat. (2026). Citation check: A new gene encoding a cytosolic glutamine synthetase in pine is linked to developing tissues. https://app.cochat.ai/papers/10.1101/2022.10.27.514046
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Valderrama-Martín, J. M., Ortigosa, F., Aledo, J. C., Ávila, C., Cánovas, F. M., & Cañas, R. A. (2022). A new gene encoding a cytosolic glutamine synthetase in pine is linked to developing tissues. The Plant Journal. https://doi.org/10.1101/2022.10.27.514046