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PreprintCited by 28Neonatal and Maternal InfectionsBacterial Genetics and BiotechnologyEscherichia coli research studies

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Heterogeneity of the group B streptococcal type VII secretion system and influence on colonization of the female genital tract

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Spencer, Brady L., Job, Alyx M., Robertson, Clare M., Hameed, Zainab A., Serchejian, Camille, Wiafe-Kwakye, Caitlin S., Mendonça, Jéssica C., Apolonio, Morgan A., Nagao, Prescilla E., Neely, Melody N., Korotkova, Natalia, Korotkov, Konstantin V., Patras, Kathryn A., Doran, Kelly S.·2023·Molecular Microbiology
DOI 10.1101/2023.01.25.525443PMC PMC10527989

Preprint — published as 10.1111/mmi.15115 · Paper page

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Abstract

SUMMARY: Type VIIb secretion systems (T7SSb) in Gram-positive bacteria facilitate physiology, interbacterial competition, and/or virulence via EssC ATPase-driven secretion of small ɑ-helical proteins and toxins. Recently, we characterized T7SSb in group B Streptococcus (GBS), a leading cause of infection in newborns and immunocompromised adults. GBS T7SS comprises four subtypes based on variation in the C-terminus of EssC and the repertoire of downstream effectors; however, the intra-species diversity of GBS T7SS and impact on GBS-host interactions remains unknown. Bioinformatic analysis indicates that GBS T7SS loci encode subtype-specific putative effectors, which have low inter-species and inter-subtype homology but contain similar domains/motifs and therefore may serve similar functions. We further identify orphaned GBS WXG100 proteins. Functionally, we show that GBS T7SS subtype I and III strains secrete EsxA in vitro and that in subtype I strain CJB111, esxA1 appears to be differentially transcribed from the T7SS operon. Further, we observe subtype-specific effects of GBS T7SS on host colonization, as subtype I but not subtype III T7SS promotes GBS vaginal persistence. Finally, we observe that T7SS subtypes I and II are the predominant subtypes in clinical GBS isolates. This study highlights the potential impact of T7SS heterogeneity on host-GBS interactions.

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Metadata

Authors
Spencer, B. L., Job, A. M., Robertson, C. M., Hameed, Z. A., Serchejian, C., Wiafe-Kwakye, C. S., Mendonça, J. C., Apolonio, M. A., Nagao, P. E., Neely, M. N., Korotkova, N., Korotkov, K. V., Patras, K. A., & Doran, K. S.
Affiliations
  • University of Colorado Anschutz (US)
  • Baylor College of Medicine (US)
  • University of Arizona (US)
  • Universidade do Estado do Rio de Janeiro (BR)
  • University of Maine (US)
  • University of Kentucky (US)
Corresponding author
Doran, Kelly S.
ORCID
Venue
Molecular Microbiology
Publisher
openRxiv
Published
2023-01-25
Volume
120
Issue
2
Pages
258-275
ISSN
0950-382X (ISSN-L)
DOI
10.1101/2023.01.25.525443
PMC
PMC10527989
OpenAlex
W4382010506
Cited by
28
References
1
Citations by year
2026: 4 · 2025: 7 · 2024: 11 · 2023: 6
FWCI
7.17
Type
journal article
Language
en
Open access
Licence
cc-by-nc
Locations
  • Molecular Microbiology (publishedVersion)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
  • PubMed Central (submittedVersion, cc-by-nc)
  • Digital Commons-TMC (Texas Medical Center) (submittedVersion)
Funders
  • National Science Foundation (2149582)
  • Coordenação de Aperfeiçoamento de Pessoal de Nível Superior ((Code 001))
  • National Institutes of Health (NIAID R21, NIAID-R01)
  • National Institute of Allergy and Infectious Diseases (R21AI169231, F32AI143203, AI169231, AI153332, R01AI153332)
  • National Institute of Neurological Disorders and Stroke (R01NS116716, NS116716)
Topics
  • Health Sciences › Medicine › Pediatrics, Perinatology and Child Health › Neonatal and Maternal Infections
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Immunology and Microbiology › Microbiology › Escherichia coli research studies
Keywords
Streptococcus agalactiae, type VII secretion system, ESAT-6, vaginal colonization, host-pathogen interaction, intraspecific variation, effectors, clinical isolates

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