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PreprintCited by 0Polysaccharides and Plant Cell WallsPlant Molecular Biology ResearchPlant Gene Expression Analysis

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Cell type-specific control of secondary cell wall formation by Musashi-type translational regulators in Arabidopsis

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Kairouani, Alicia, Pontier, Dominique, Picart, Claire, Mounet, Fabien, Martinez, Yves, Le-Bot, Lucie, Fanuel, Mathieu, Hammann, Philippe, Belmudes, Lucid, Merret, Rémy, Azevedo, Jacinthe, Carpentier, Marie-Christine, Gagliardi, Dominique, Couté, Yohann, Sibout, Richard, Bies-Etheve, Natacha, Lagrange, Thierry·2023·bioRxiv (Cold Spring Harbor Laboratory)
DOI 10.1101/2023.03.20.532888

Preprint — published as 10.7554/elife.88207.3 · Paper page

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Abstract

Deciphering the mechanism of secondary cell wall/SCW formation in plants is key to understanding their development and the molecular basis of biomass recalcitrance. Although transcriptional regulation is essential for SCW formation, little is known about the implication of post-transcriptional mechanisms in this process. Here we report that two bonafide RNA-binding proteins homologous to the animal translational regulator Musashi, MSIL2 and MSIL4, function redundantly to control SCW formation in Arabidopsis. MSIL2/4 interactomes are similar and enriched in proteins involved in mRNA binding and translational regulation. MSIL2/4 mutations alter SCW formation in the fibers, leading to a reduction in lignin deposition, and an increase of 4- O -glucuronoxylan methylation. In accordance, quantitative proteomics of stems reveal an overaccumulation of glucuronoxylan biosynthetic machinery, including GXM3, in the msil2/4 mutant stem. We showed that MSIL4 immunoprecipitates GXM mRNAs, suggesting a novel aspect of SCW regulation, linking post-transcriptional control to the regulation of SCW biosynthesis genes.

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Metadata

Authors
Kairouani, A., Pontier, D., Picart, C., Mounet, F., Martinez, Y., Le-Bot, L., Fanuel, M., Hammann, P., Belmudes, L., Merret, R., Azevedo, J., Carpentier, M.-C., Gagliardi, D., Couté, Y., Sibout, R., Bies-Etheve, N., & Lagrange, T.
Affiliations
  • Centre National de la Recherche Scientifique (FR)
  • Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
Corresponding author
Lagrange, Thierry
ORCID
Venue
bioRxiv (Cold Spring Harbor Laboratory)
Publisher
openRxiv
Published
2023-03-20
DOI
10.1101/2023.03.20.532888
OpenAlex
W4327915427
Cited by
0
References
1
Type
posted content
Language
en
Open access
Locations
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
  • HAL (submittedVersion, cc-by)
Funders
  • Agence Nationale de la Recherche (ANR-10-INBS-08, ANR-17-EURE-0003, ANR-12-BSV6-0010, ANR-17-EURE-, Proteomics French Infrastructure,ANR-10-INBS-08, ANR18, ANR-08-BLAN-0206, ANR-17, ANR-10, ANR-18-EURE-0019)
  • Centre National de la Recherche Scientifique
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Polysaccharides and Plant Cell Walls
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant Gene Expression Analysis
Keywords
secondary cell wall formation, RNA-binding proteins, post-transcriptional regulation, lignification, glucuronoxylan, xylan biosynthesis, cell type-specific gene regulation, Arabidopsis thaliana, stem fibers, protein-protein interaction

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