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PreprintCited by 1DNA Repair MechanismsGenomics and Phylogenetic StudiesOrigins and Evolution of Life

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Metagenome mining and functional analysis reveal oxidized guanine DNA repair at the Lost City Hydrothermal Field

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Utzman, Payton H., Mays, Vincent P., Miller, Briggs C., Fairbanks, Mary C., Brazelton, William J., Horvath, Martin P.·2023·PLoS ONE
DOI 10.1101/2023.04.05.535768PMC PMC11078426

Preprint — published as 10.1371/journal.pone.0284642

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Abstract

The GO DNA repair system protects against GC → TA mutations by finding and removing oxidized guanine. The system is mechanistically well understood but its origins are unknown. We searched metagenomes and abundantly found the genes encoding GO DNA repair at the Lost City Hydrothermal Field (LCHF). We recombinantly expressed the final enzyme in the system to show MutY homologs function to suppress mutations. Microbes at the LCHF thrive without sunlight, fueled by the products of geochemical transformations of seafloor rocks, under conditions believed to resemble a young Earth. High levels of the reductant H 2 and low levels of O 2 in this environment raise the question, why are resident microbes equipped to repair damage caused by oxidative stress? MutY genes could be assigned to metagenome assembled genomes (MAGs), and thereby associate GO DNA repair with metabolic pathways that generate reactive oxygen, nitrogen and sulfur species. Our results indicate that cell-based life was under evolutionary pressure to cope with oxidized guanine well before O 2 levels rose following the great oxidation event.

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Metadata

Authors
Utzman, P. H., Mays, V. P., Miller, B. C., Fairbanks, M. C., Brazelton, W. J., & Horvath, M. P.
Affiliations
University of Utah (US)
Corresponding author
Horvath, Martin P.
ORCID
Venue
PLoS ONE
Publisher
openRxiv
Published
2023-04-05
Volume
19
Issue
5
Pages
e0284642-e0284642
ISSN
1932-6203 (ISSN-L)
DOI
10.1101/2023.04.05.535768
PMC
PMC11078426
OpenAlex
W4396733631
Cited by
1
References
1
Citations by year
2026: 1
FWCI
0.14
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • PLoS ONE (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • National Science Foundation (2204229, 1905249, 1536405)
  • National Aeronautics and Space Administration
  • University of Utah
  • NASA Astrobiology Institute
  • Division of Chemistry
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
  • Physical Sciences › Physics and Astronomy › Astronomy and Astrophysics › Origins and Evolution of Life
Keywords
Lost City hydrothermal field, metagenome mining, metagenome-assembled genomes, reductive metabolism, reactive oxygen species, Great Oxidation Event, origin of life, oxidative stress
MeSH terms
DNA Repair, Guanine, Oxidation-Reduction, Metagenome, Hydrothermal Vents

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