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Substrate specificity and functional conservation of SWEET10 transporter in pineapple
Open accessPreprint — published as 10.1093/hr/uhad175
Abstract
Summary: SWEET transporters are a unique class of sugar transporters that play vital roles in various developmental and physiological processes in plants. While the functions of SWEETs have been well established in model plants such as Arabidopsis , their functions in economically important fruit crops like pineapple have not been well studied. This study investigated the substrate specificity of pineapple SWEETs by comparing the protein sequences of known glucose and sucrose transporters in Arabidopsis to those in pineapple. Genome-wide approach and 3D structure comparison showed that the Arabidopsis SWEET8 homolog in pineapple, AcSWEET10, shares similar sequences and protein properties responsible for glucose transport. To determine the functional conservation of AcSWEET10, its ability to complement glucose transport mutants in yeast, its expression in stamens, and its impact on the microspore phenotype and seed set in transgenic Arabidopsis were analyzed. AcSWEET10 was found to be functionally equivalent to AtSWEET8 and plays a critical role in regulating microspore formation through the regulation of CalS5. Manipulating SWEET transporter activity could have important implications for improving fruit crop yield and quality.
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Highly accurate protein structure prediction with AlphaFold
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Metadata
- Authors
- Fakher, B., Ashraf, M. A., Wang, L., Wang, X., Zheng, P., Aslam, M., & Qin, Y.
- Affiliations
- Guangxi University (CN)
- Howard University (US)
- Guangxi Academy of Agricultural Science (CN)
- Donald Danforth Plant Science Center (US)
- Corresponding author
- Aslam, Mohammad
- Qin, Yuan
- Venue
- Horticulture Research
- Publisher
- openRxiv
- Published
- 2023-04-06
- Volume
- 10
- Issue
- 10
- Pages
- uhad175-uhad175
- ISSN
- 2052-7276 (ISSN-L)
- PMC
- PMC10660354
- OpenAlex
- W4386365914
- Cited by
- 18
- References
- 1
- Citations by year
- 2026: 5 · 2025: 8 · 2024: 5
- FWCI
- 2.84
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- Horticulture Research (publishedVersion, cc-by)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- Shanghai Normal University
- Fujian Agriculture and Forestry University (VW4000)
- Guangxi Academy of Agricultural Sciences
- Science and Technology Major Project of Guangxi (Gui Ke AA22068096)
- Modern Agricultural Technology Industry System of Shandong province
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant nutrient uptake and metabolism
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant Reproductive Biology
- Keywords
- SWEET transporters, pineapple, glucose transporters, sucrose transporters, substrate specificity, microspore formation, seed set, stamen development, functional complementation, transgenic Arabidopsis, fruit crop yield
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