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PreprintCited by 15RNA Research and SplicingCancer-related Molecular PathwaysSarcoma Diagnosis and Treatment

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

The RNA binding proteins LARP4A and LARP4B promote sarcoma and carcinoma growth and metastasis

Open access
Coleman, Jennifer C., Tattersall, Luke, Yianni, Val, Knight, Laura, Yu, Hongqiang, Hallett, Sadie, Johnson, Philip, Caetano, Ana, Cosstick, Charlie, Ridley, Anne, Gartland, Alison, Conte, Maria R, Grigoriadis, Agamemnon E.·2023·iScience
DOI 10.1101/2023.04.11.536377PMC PMC10963253

Preprint — published as 10.1016/j.isci.2024.109288 · Paper page

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Abstract

SUMMARY: RNA-binding proteins (RBPs) are emerging as important regulators of pathogenesis, including cancer. Here we reveal that the recently characterised RBPs LARP4A and LARP4B are differentially overexpressed in primary osteosarcoma and osteosarcoma lung metastases, as well as in prostate cancer. Depletion of LARP4A and LARP4B inhibited primary tumour growth and metastatic spread in xenograft studies, as well as inhibiting cell proliferation, motility and migration. Transcriptomic profiling combined with high content multiparametric cell cycle analysis unveiled a central role for LARP4B, but not LARP4A, in regulating cell cycle progression in osteosarcoma and prostate cancer cell lines, potentially through modulating the post-transcriptional regulation of RNA targets that include key cell cycle proteins such as Cyclins B1 and E2, Aurora B and E2F1. Our work assigns new functions to LARP4A and LARP4B as pro-tumorigenic proteins in bone and prostate cancer, highlights their similarities while indicating distinct functional aspects, and adds significantly to the rapidly increasing roles of RBPs in different cancer models. Uncovering clear biological roles for these paralogous proteins provides new avenues for identifying novel tissue-specific targets and potential druggable intervention.

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  1. 10.1016/j.cell.2018.02.060 · DOI · Paper page
  2. 10.1016/j.cell.2011.02.013 · DOI · Paper page
  3. 10.1016/j.cell.2009.02.007 · DOI
  4. 10.1038/nrg3434 · DOI
  5. 10.1016/j.cell.2012.04.031 · DOI · Paper page
  6. 10.1038/s41586-020-2077-3 · DOI · Paper page
  7. 10.1172/JCI151627 · DOI
  8. 10.1016/j.tig.2008.05.004 · DOI
  9. 10.3390/cancers12092699 · DOI
  10. 10.1002/wrna.1430 · DOI
  11. 10.1261/rna.1478709 · DOI
  12. 10.1080/15476286.2019.1695712 · DOI
  13. 10.1080/15476286.2020.1733799 · DOI
  14. 10.1093/nar/gku1287 · DOI
  15. 10.1016/j.str.2008.02.021 · DOI
  16. 2004, Structural analysis of cooperative RNA binding by the La motif and central RRM domain of human La protein, Nature structural & molecular biology, 11, 323, 10.1038/nsmb747 · DOI
  17. 10.1093/nar/gkv173 · DOI
  18. 10.1093/nar/gkac696 · DOI
  19. 10.1093/nar/gkz144 · DOI
  20. 10.3390/biom5042701 · DOI
  21. 10.3390/biom10060872 · DOI
  22. 10.1261/rna.2146910 · DOI
  23. 10.1128/MCB.01162-10 · DOI
  24. 10.1261/rna.051441.115 · DOI
  25. 10.7554/elife.28889 · DOI
  26. 10.7554/elife.59186 · DOI
  27. 10.1093/nar/gkaa643 · DOI
  28. 10.1038/s41467-021-25078-5 · DOI
  29. 10.1074/jbc.RA120.013454 · DOI
  30. 10.1016/j.bbrc.2018.02.148 · DOI
  31. 10.1080/15476286.2020.1739930 · DOI
  32. 10.1261/rna.035469.112 · DOI
  33. 2011, Identification and characterization of a set of conserved and new regulators of cytoskeletal organization, cell morphology and migration, BMC biology, 9, 1
  34. 10.1620/tjem.247.59 · DOI
  35. 10.1002/cm.21336 · DOI
  36. 10.1073/pnas.1215899109 · DOI
  37. 10.1158/0008-5472.CAN-15-2308 · DOI
  38. 10.1016/j.exphem.2014.12.003 · DOI
  39. 10.1155/2019/1569049 · DOI
  40. 10.1038/ng.3175 · DOI
  41. 10.1038/ng.2847 · DOI
  42. 10.1038/ng.2641 · DOI
  43. 10.1038/nature10825 · DOI
  44. 10.1038/ncomms15943 · DOI
  45. 10.1083/jcb.122.3.685 · DOI
  46. 10.1038/onc.2015.344 · DOI
  47. 10.1186/s12920-018-0463-6 · DOI
  48. 10.1016/j.jbo.2021.100398 · DOI
  49. 10.1371/journal.pone.0134306 · DOI
  50. 10.7554/elife.50796 · DOI
  51. 10.3389/fendo.2017.00311 · DOI
  52. 10.1038/s41418-021-00823-x · DOI
  53. 10.1186/1476-4598-9-50 · DOI
  54. Lewis, B.M. , Cho, C.Y. , Her, H.-L. , Hunter, T. , and Yeo, G.W. (2022). LARP4 Is an RNA-Binding Protein That Binds Nuclear-Encoded Mitochondrial mRNAs To Promote Mitochondrial Function. Cold Spring Harbor Laboratory. · DOI
  55. 10.1530/ERC-20-0517 · DOI
  56. 10.2174/1381612822666160512151028 · DOI
  57. 10.3389/fonc.2015.00278 · DOI
  58. 2014, Knockdown of Aurora-B inhibits osteosarcoma cell invasion and migration via modulating PI3K/Akt/NF-kappaB signaling pathway, Int J Clin Exp Pathol, 7, 3984
  59. 10.1002/pros.20345 · DOI
  60. 10.1038/nrc3838 · DOI · Paper page
  61. 10.1016/j.celrep.2020.107669 · DOI
  62. 10.1038/s41598-022-14436-y · DOI
  63. 10.1016/j.gde.2017.11.004 · DOI
  64. 10.1016/j.semcdb.2014.05.014 · DOI
  65. 10.1186/2049-3002-2-7 · DOI
  66. 10.18632/oncotarget.2766 · DOI
  67. 10.1016/j.cmet.2021.04.002 · DOI
  68. 10.7554/eLife.37663 · DOI
  69. 10.1038/ncomms11212 · DOI
  70. 10.3389/fcell.2019.00355 · DOI
  71. 10.1016/j.tem.2017.06.004 · DOI
  72. 10.1002/jcp.30729 · DOI
  73. 10.3389/fmolb.2021.751842 · DOI
  74. 10.1016/j.ygeno.2020.08.034 · DOI
  75. 10.1038/s41598-017-17204-5 · DOI · Paper page
  76. 10.1111/j.1365-2818.2006.01706.x · DOI
  77. 10.1038/nmeth.2089 · DOI · Paper page
  78. Goldman, M. , Craft, B. , Hastie, M. , Repečka, K. , McDade, F. , Kamath, A. , Banerjee, A. , Luo, Y. , Rogers, D. , and Brooks, A.N. (2018). The UCSC Xena platform for public and private cancer genomics data visualization and interpretation. biorxiv, 326470. · DOI
  79. 10.1038/s41587-020-0546-8 · DOI · Paper page
  80. 10.1101/gr.229102. Article published online before print in May 2002 · DOI · Paper page
  81. 10.1038/nmeth.3317 · DOI · Paper page
  82. 10.1093/bioinformatics/btt656 · DOI · Paper page
  83. 10.1093/nar/gkv412 · DOI
  84. 10.1093/bioinformatics/btp616 · DOI · Paper page
  85. 10.1186/1471-2105-14-128 · DOI · Paper page
  86. 10.1093/nar/gkw377 · DOI · Paper page
  87. 10.1002/cpz1.90 · DOI · Paper page

Metadata

Authors
Coleman, J. C., Tattersall, L., Yianni, V., Knight, L., Yu, H., Hallett, S., Johnson, P., Caetano, A., Cosstick, C., Ridley, A., Gartland, A., Conte, M. R., & Grigoriadis, A. E.
Affiliations
  • King's College London (GB)
  • University of Sheffield (GB)
  • University of Bristol (GB)
Corresponding author
  • Conte, Maria R
  • Grigoriadis, Agamemnon E.
ORCID
Venue
iScience
Publisher
openRxiv
Published
2023-04-12
Volume
27
Issue
4
Pages
109288-109288
ISSN
2589-0042 (ISSN-L)
DOI
10.1101/2023.04.11.536377
PMC
PMC10963253
OpenAlex
W4392131697
Cited by
15
References
1
Citations by year
2026: 2 · 2025: 8 · 2024: 3 · 2023: 1
FWCI
1.75
Type
journal article
Language
en
Open access
Licence
cc-by-nc-nd
Locations
  • iScience (publishedVersion, cc-by-nc-nd)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
  • PubMed Central (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • White Rose Research Online (University of Leeds, The University of Sheffield, University of York) (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
  • Research Portal (King's College London) (submittedVersion, cc-by)
Funders
  • King’s College London
  • Francis Crick Institute
  • King's College London (MR/N013700/1)
  • National Institutes of Health
  • Medical Research Council (MR/N013700/1)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
  • Health Sciences › Medicine › Oncology › Cancer-related Molecular Pathways
  • Health Sciences › Medicine › Oncology › Sarcoma Diagnosis and Treatment
Keywords
LARP4B, RNA-binding proteins, osteosarcoma, prostate cancer, lung metastasis, tumor growth, cell cycle regulation, cyclin B1, cyclin E2, Aurora B kinase, E2F1, cell migration, cell proliferation, paralogous proteins

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