Cited by 2,132RNA Research and SplicingRNA and protein synthesis mechanismsBiotin and Related Studies

Insights into RNA Biology from an Atlas of Mammalian mRNA-Binding Proteins

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Castello, Alfredo, Fischer, Bernd, Eichelbaum, Katrin, Horos, Rastislav, Beckmann, Benedikt M., Strein, Claudia, Davey, Norman E., Humphreys, David T. +4 more·2012·Cell

Abstract

RNA-binding proteins (RBPs) determine RNA fate from synthesis to decay. Employing two complementary protocols for covalent UV crosslinking of RBPs to RNA, we describe a systematic, unbiased, and comprehensive approach, termed "interactome capture," to define the mRNA interactome of proliferating human HeLa cells. We identify 860 proteins that qualify as RBPs by biochemical and statistical criteria, adding more than 300 RBPs to those previously known and shedding light on RBPs in disease, RNA-binding enzymes of intermediary metabolism, RNA-binding kinases, and RNA-binding architectures. Unexpectedly, we find that many proteins of the HeLa mRNA interactome are highly intrinsically disordered and enriched in short repetitive amino acid motifs. Interactome capture is broadly applicable to study mRNA interactome composition and dynamics in varied biological settings.

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Crossref · 10.1016/j.cell.2012.04.031

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  • Many enzymes, including those connected with the TCA cycle, glycolysis, PPP, fatty acid metabolism, and other pathways, have been shown to bind RNA in vitro and in cultured cells.

    “Many enzymes, including those connected with the TCA cycle, glycolysis, PPP, fatty acid metabolism, and other pathways, have been shown to bind RNA in vitro and in cultured cells (105, 106).”

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Authors
Castello, A., Fischer, B., Eichelbaum, K., Horos, R., Beckmann, B. M., Strein, C., Davey, N. E., Humphreys, D. T., Preiss, T., Steinmetz, L. M., Krijgsveld, J., & Hentze, M. W.
Affiliations
  • European Molecular Biology Laboratory (DE)
  • Victor Chang Cardiac Research Institute (AU)
  • Australian National University (AU)
Corresponding author
  • Krijgsveld, Jeroen
  • Hentze, Matthias W.
Venue
Cell
Publisher
Elsevier BV
Published
2012-06
Volume
149
Issue
6
Pages
1393-1406
ISSN
0092-8674 (ISSN-L)
OpenAlex
W2041440237
Cited by
2,132
References
71
Citations by year
2026: 97 · 2025: 131 · 2024: 170 · 2023: 163 · 2022: 164 · 2021: 196 · 2020: 175 · 2019: 168 · 2018: 155 · 2017: 167 · 2016: 171 · 2015: 146 · 2014: 118 · 2013: 84 · 2012: 26
FWCI
48.12
Type
journal article
Language
en
Open access
Locations
  • Cell (publishedVersion)
  • PubMed (publishedVersion)
  • ENLIGHTEN (Jurnal Bimbingan dan Konseling Islam) (submittedVersion)
  • ANU Open Research (Australian National University) (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Biotin and Related Studies
Keywords
RNA-binding proteins, UV crosslinking, HeLa cells, intermediary metabolism, intrinsically disordered proteins
MeSH terms
Animals, HeLa Cells, Humans, RNA, Messenger, RNA-Binding Proteins, Proteomics

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