Insights into RNA Biology from an Atlas of Mammalian mRNA-Binding Proteins
Open accessAbstract
RNA-binding proteins (RBPs) determine RNA fate from synthesis to decay. Employing two complementary protocols for covalent UV crosslinking of RBPs to RNA, we describe a systematic, unbiased, and comprehensive approach, termed "interactome capture," to define the mRNA interactome of proliferating human HeLa cells. We identify 860 proteins that qualify as RBPs by biochemical and statistical criteria, adding more than 300 RBPs to those previously known and shedding light on RBPs in disease, RNA-binding enzymes of intermediary metabolism, RNA-binding kinases, and RNA-binding architectures. Unexpectedly, we find that many proteins of the HeLa mRNA interactome are highly intrinsically disordered and enriched in short repetitive amino acid motifs. Interactome capture is broadly applicable to study mRNA interactome composition and dynamics in varied biological settings.
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Many enzymes, including those connected with the TCA cycle, glycolysis, PPP, fatty acid metabolism, and other pathways, have been shown to bind RNA in vitro and in cultured cells.
“Many enzymes, including those connected with the TCA cycle, glycolysis, PPP, fatty acid metabolism, and other pathways, have been shown to bind RNA in vitro and in cultured cells (105, 106).”
From Fueling Immunity: Insights into Metabolism and Lymphocyte Function (2013) · cites this paper as [105]
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Schmeing, S., & Hart, P. '. (2024) · WIREs RNA · doi:10.1002/wrna.1877 · Cited by 11 · see citing works
He, J., Zhou, T., Hu, L.-F., Jiao, Y., Wang, J., Yan, S., Jia, S., Chen, Q., Zhu, W., Zhang, J., et al. (2026) · Nature Communications · doi:10.1038/s41467-026-72351-6
- Authors
- Castello, A., Fischer, B., Eichelbaum, K., Horos, R., Beckmann, B. M., Strein, C., Davey, N. E., Humphreys, D. T., Preiss, T., Steinmetz, L. M., Krijgsveld, J., & Hentze, M. W.
- Affiliations
- European Molecular Biology Laboratory (DE)
- Victor Chang Cardiac Research Institute (AU)
- Australian National University (AU)
- Corresponding author
- Krijgsveld, Jeroen
- Hentze, Matthias W.
- ORCID
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2012-06
- Volume
- 149
- Issue
- 6
- Pages
- 1393-1406
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2041440237
- Cited by
- 2,132
- References
- 71
- Citations by year
- 2026: 97 · 2025: 131 · 2024: 170 · 2023: 163 · 2022: 164 · 2021: 196 · 2020: 175 · 2019: 168 · 2018: 155 · 2017: 167 · 2016: 171 · 2015: 146 · 2014: 118 · 2013: 84 · 2012: 26
- FWCI
- 48.12
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- ENLIGHTEN (Jurnal Bimbingan dan Konseling Islam) (submittedVersion)
- ANU Open Research (Australian National University) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Biotin and Related Studies
- Keywords
- RNA-binding proteins, UV crosslinking, HeLa cells, intermediary metabolism, intrinsically disordered proteins
- MeSH terms
- Animals, HeLa Cells, Humans, RNA, Messenger, RNA-Binding Proteins, Proteomics
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