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PreprintCited by 24RNA Research and SplicingRNA and protein synthesis mechanismsReproductive Biology and Fertility

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eIF4E1b is a non-canonical eIF4E required for maternal mRNA dormancy

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Lorenzo-Orts, Laura, Strobl, Marcus, Steinmetz, Benjamin, Leesch, Friederike, Pribitzer, Carina, Schutzbier, Michael, Dürnberger, Gerhard, Pauli, Andrea·2023·EMBO Reports
DOI 10.1101/2023.06.10.544440PMC PMC10883267

Preprint — published as 10.1038/s44319-023-00006-4 · Paper page

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Abstract

Maternal mRNAs are essential for protein synthesis during oogenesis and early embryogenesis. To adapt translation to specific needs during development, maternal mRNAs are translationally repressed by shortening the polyA tails. While mRNA deadenylation is associated with decapping and degradation in somatic cells, maternal mRNAs with short polyA tails are stable. Here we report an essential role for the germline-specific paralog of the mRNA cap-binding factor eIF4E, known as eIF4E1b, in the storage and repression of maternal mRNAs with short polyA tails. eIF4E1b binds to the mRNA cap and is targeted to ribonucleoprotein complexes through its direct interaction with eIF4ENIF1/4E-T. In early embryos, eIF4E1b binds to a specific set of translationally repressed mRNAs with short or no polyA tails, such as histone mRNAs, which are translated later on during embryogenesis. Consistent with an important role in maternal mRNA dormancy, mutation of eIF4E1b in zebrafish impairs female germline development. Understanding the mechanism and function of eIF4E1B provides new insights into fundamental post-transcriptional regulatory principles governing early vertebrate development.

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Metadata

Authors
Lorenzo-Orts, L., Strobl, M., Steinmetz, B., Leesch, F., Pribitzer, C., Schutzbier, M., Dürnberger, G., & Pauli, A.
Affiliations
  • Research Institute of Molecular Pathology (AT)
  • University of Vienna (AT)
Corresponding author
Pauli, Andrea
ORCID
Venue
EMBO Reports
Publisher
openRxiv
Published
2023-06-10
Volume
25
Issue
1
Pages
404-427
ISSN
1469-221X (ISSN-L)
DOI
10.1101/2023.06.10.544440
PMC
PMC10883267
OpenAlex
W4389941098
Cited by
24
References
1
Citations by year
2026: 5 · 2025: 9 · 2024: 10
FWCI
2.12
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • EMBO Reports (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Repository for Publications and Research Data (ETH Zurich) (submittedVersion, cc-by)
  • ETH Zürich Research Collection
Funders
  • Research Institute of Molecular Pathology
  • European Molecular Biology Organization (ALTF 1165-2019)
  • Boehringer Ingelheim
  • European Commission (890218)
  • Human Frontier Science Program (CDA00066/2015 and RGY0079/2020)
  • Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (191204)
  • Österreichischen Akademie der Wissenschaften
  • Austrian Science Fund (1031-B28, Y 1031, SFB RNADeco (F80))
  • Österreichische Forschungsförderungsgesellschaft (FFG-852936 and Y 1031-B28)
  • University of California, Davis
  • European Research Council (101044495/GaMe)
  • HORIZON EUROPE Marie Sklodowska-Curie Actions (890218)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Health Sciences › Medicine › Reproductive Medicine › Reproductive Biology and Fertility
Keywords
maternal mRNA, mRNA storage, P-bodies, mRNA deadenylation, histone mRNA, eIF4G, oogenesis, germline, post-transcriptional regulation, zebrafish
MeSH terms
Animals, Histones, Humans, RNA, Messenger, Protein Biosynthesis, Zebrafish, RNA, Messenger, Stored, Eukaryotic Initiation Factor-4E, Mice

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