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Multi-organ Metabolic Model of Zea mays Connects Temperature Stress with Thermodynamics-Reducing Power-Energy Generation Axis
Open accessPreprint — published as 10.1016/j.isci.2023.108400 · Paper page
Abstract
Global climate change has severely impacted maize productivity. A holistic understanding of metabolic crosstalk among its organs is essential to address this issue. Thus, we reconstructed the first multi-organ maize genome-scale metabolic model, i ZMA6517, and contextualized it with heat and cold stress-related transcriptomics data using the novel EX pression dis T ributed REA ction flux M easurement (EXTREAM) algorithm. Furthermore, implementing metabolic bottleneck analysis on contextualized models revealed fundamental differences between these stresses. While both stresses had reducing power bottlenecks, heat stress had additional energy generation bottlenecks. To tie these signatures, we performed thermodynamic driving force analysis, revealing thermodynamics-reducing power-energy generation axis dictating the nature of temperature stress responses. Thus, for global food security, a temperature-tolerant maize ideotype can be engineered by leveraging the proposed thermodynamics-reducing power-energy generation axis. We experimentally inoculated maize root with a beneficial mycorrhizal fungus, Rhizophagus irregularis , and as a proof of concept demonstrated its potential to alleviate temperature stress. In summary, this study will guide the engineering effort of temperature stress-tolerant maize ideotypes.
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Metadata
- Authors
- Chowdhury, N. B., Decouard, B., Quillere, I., Rigault, M., Sajeevan, K. A., Acharya, B., Chowdhury, R., Hirel, B., Dellagi, A., Maranas, C., & Saha, R.
- Affiliations
- University of Nebraska–Lincoln (US)
- AgroParisTech (FR)
- Iowa State University (US)
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
- Pennsylvania State University (US)
- Corresponding author
- Saha, Rajib
- ORCID
- Venue
- iScience
- Publisher
- openRxiv
- Published
- 2023-07-10
- Volume
- 26
- Issue
- 12
- Pages
- 108400-108400
- ISSN
- 2589-0042 (ISSN-L)
- PMC
- PMC10709110
- OpenAlex
- W4388461873
- Cited by
- 25
- References
- 1
- Citations by year
- 2026: 10 · 2025: 11 · 2024: 3 · 2023: 1
- FWCI
- 2.23
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by-nc-nd
- Locations
- iScience (publishedVersion, cc-by-nc-nd)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nd)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- HAL (submittedVersion, cc-by-nc-nd)
- Lincoln (University of Nebraska) (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) (submittedVersion)
- Funders
- National Science Foundation (1943310)
- U.S. Department of Energy
- Iowa State University (2242763, RII Track-1)
- Agence Nationale de la Recherche (ANR-11-IDEX-0003)
- Institut National de la Recherche Agronomique (IB BAP 2021 MYCORN)
- Institut National de Recherche en Sciences et Technologies pour l'Environnement et l'Agriculture (IB BAP 2021 MYCORN)
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
- Office of Science
- Biological and Environmental Research
- Center for Bioenergy Innovation
- Vermont Agency of Natural Resources (18-CARN-024-01 \u2013 2018, 18-CARN-024-01 – 2018)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bioinformatics and Genomic Networks
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant nutrient uptake and metabolism
- Keywords
- maize, temperature stress, heat stress, cold stress, reducing power, energy production, metabolic crosstalk, transcriptomics, Glomus intraradices, mycorrhizal fungi, climate change
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