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PreprintCited by 37Photosynthetic Processes and MechanismsPlant biochemistry and biosynthesisAlgal biology and biofuel production

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A promiscuous mechanism to phase separate eukaryotic carbon fixation in the green lineage

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Barrett, James, Naduthodi, Mihris I.S., Mao, Yuwei, Dégut, Clément, Musiał, Sabina, Salter, Aidan, Leake, Mark C., Plevin, Michael J., McCormick, Alistair J., Blaza, James N., Mackinder, Luke C.M.·2024·Nature Plants
DOI 10.1101/2024.04.09.588658PMC PMC11570498

Preprint — published as 10.1038/s41477-024-01812-x · Paper page

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Abstract

CO 2 fixation is commonly limited by inefficiency of the CO 2 -fixing enzyme Rubisco. Eukaryotic algae concentrate and fix CO 2 in phase-separated condensates called pyrenoids, which complete up to one-third of global CO 2 fixation. Condensation of Rubisco in pyrenoids is dependent on interaction with disordered linker proteins that show little conservation between species. We developed a sequence-independent bioinformatic pipeline to identify linker proteins in green algae. We report the linker from Chlorella and demonstrate that it binds a conserved site on the Rubisco large subunit. We show the Chlorella linker phase separates Chlamydomonas Rubisco and that despite their separation by ∼800 million years of evolution, the Chlorella linker can support the formation of a functional pyrenoid in Chlamydomonas . This cross-species reactivity extends to plants, with the Chlorella linker able to drive condensation of some native plant Rubiscos in vitro and in planta . Our results represent an exciting frontier for pyrenoid engineering in plants, which is modelled to increase crop yields.

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Metadata

Authors
Barrett, J., Naduthodi, M. I. S., Mao, Y., Dégut, C., Musiał, S., Salter, A., Leake, M. C., Plevin, M. J., McCormick, A. J., Blaza, J. N., & Mackinder, L. C. M.
Affiliations
  • University of York (GB)
  • University of Edinburgh (GB)
Corresponding author
Mackinder, Luke C.M.
ORCID
Venue
Nature Plants
Publisher
openRxiv
Published
2024-04-11
Volume
10
Issue
11
Pages
1801-1813
ISSN
2055-026X (ISSN-L)
DOI
10.1101/2024.04.09.588658
PMC
PMC11570498
OpenAlex
W4403253348
Cited by
37
References
1
Citations by year
2026: 20 · 2025: 11 · 2024: 6
FWCI
5.04
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Nature Plants (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • White Rose Research Online (University of Leeds, The University of Sheffield, University of York) (submittedVersion)
  • Edinburgh Research Explorer (University of Edinburgh) (submittedVersion, cc-by)
Funders
  • National Science Foundation
  • Bill and Melinda Gates Foundation (INV-054558)
  • Wellcome Trust (206161, Z/17/Z, 206161/Z/17/Z)
  • UK Research and Innovation (MR/T040742/1)
  • Research Councils UK (MR/T040742/1, EP/W024063/1)
  • Nederlandse Organisatie voor Wetenschappelijk Onderzoek (019.221EN.010)
  • Darwin Trust of Edinburgh
  • Directorate for Biological Sciences
  • Engineering and Physical Sciences Research Council (EP/W024063/1)
  • Biotechnology and Biological Sciences Research Council (BB/S015337/1, BB/M0111511a, BB/S015337/11, BB/Y000323/1, BB/T007222/1)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
Keywords
pyrenoid, Rubisco, phase separation, linker proteins, CO2 fixation, Chlorella, Chlamydomonas, green algae, Rubisco large subunit, protein condensates, crop yield, CO2-concentrating mechanism
MeSH terms
Carbon Dioxide, Chlamydomonas, Chlorella, Ribulose-Bisphosphate Carboxylase, Carbon Cycle

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