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Engineering of soluble bacteriorhodopsin
Open accessPreprint — published as 10.1039/d5sc02453f · Paper page
Abstract
Bacteriorhodopsin is a seven-helical light-driven proton pump and a model membrane protein. Here, we report engineering of soluble analogues of bacteriorhodopsin, NeuroBRs, which bind retinal and photocycle under illumination. We also report the crystallographic structure of NeuroBR_A, determined at anisotropic resolution reaching 1.76 Å, that reveals a conserved chromophore binding pocket and tertiary structure. Our results highlight the power of modern protein engineering approaches and pave the way towards wider development of molecular tools derived from membrane proteins.
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Metadata
- Authors
- Nikolaev, A., Orlov, Y., Tsybrov, F., Kuznetsova, E., Shishkin, P., Kuzmin, A., Mikhailov, A., Galochkina, Y. S., Anuchina, A., Chizhov, I., Semenov, O., Kapranov, I., Borshchevskiy, V., Remeeva, A., & Gushchin, I.
- Affiliations
- Moscow Institute of Physics and Technology (RU)
- Corresponding author
- Gushchin, Ivan
- ORCID
- Venue
- Chemical Science
- Publisher
- openRxiv
- Published
- 2024-11-21
- Volume
- 16
- Issue
- 24
- Pages
- 11067-11076
- ISSN
- 2041-6520 (ISSN-L)
- PMC
- PMC12094106
- OpenAlex
- W4410338897
- Cited by
- 6
- References
- 1
- Citations by year
- 2026: 5 · 2025: 1
- FWCI
- 2.35
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Licence
- Locations
- Chemical Science (publishedVersion, cc-by-nc)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- Funders
- Salt Science Research Foundation (BL02U1)
- Russian Science Foundation (21-64-00018)
- Ministry of Science and Higher Education of the Russian Federation (075–15‐2021–1354, -2025-0003, FSMG‐2025‐0003, 075-15-)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Photoreceptor and optogenetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
- Keywords
- bacteriorhodopsin, retinal binding pocket, chromophore binding, photocycle, crystal structure, optogenetics, light-driven proton pump
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