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Coupling chromosome organization to genome segregation in Archaea
Open accessPreprint — published as 10.1038/s41467-025-61997-3 · Paper page
Abstract
Chromosome segregation is a fundamental process in all life forms and requires coordination with genome organization, replication and cell division. The mechanism that mediates chromosome segregation in archaea remains enigmatic, despite the centre-stage role assumed by these organisms in the discourse about the origin of eukaryotes. Previously, we identified two proteins, SegA and SegB, which form a minimalist chromosome partition machine in Sulfolobales. Here we uncover patterns and mechanisms that the SegAB system employs to link chromosome organization to genome segregation. Deletion of the genes causes growth and chromosome partition defects. SegB binds to multiple sites scattered across the chromosome, but mainly localised close to the segAB locus in most archaeal genera. The sites are predominantly intragenic and enriched in one of the two compartments into which the chromosome folds. We show that SegB coalesces into multiple foci through the nucleoid, exhibiting a biased localisation towards the cell periphery, which hints at potential tethers to the cell membrane. The short-range DNA compaction activity of SegB and long-range looping of distant sites point to a significant role for SegB in chromosome condensation that in turn enables genome segregation. Collectively, our data put forward SegAB as important players in bridging chromosome organization to genome segregation in archaea.
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Metadata
- Authors
- Kabli, A. F., Ng, I. W., Read, N., Pal, P., Reimann, J., Tran, N. T., Albers, S.-V., Le, T. B. K., & Barillà, D.
- Affiliations
- University of York (GB)
- John Innes Centre (GB)
- University of Freiburg (DE)
- Corresponding author
- Barillà, Daniela
- Venue
- Nature Communications
- Publisher
- openRxiv
- Published
- 2025-02-08
- Volume
- 16
- Issue
- 1
- Pages
- 6759-6759
- ISSN
- 2041-1723 (ISSN-L)
- PMC
- PMC12284271
- OpenAlex
- W4412559258
- Cited by
- 10
- References
- 1
- Citations by year
- 2026: 4 · 2025: 6
- FWCI
- 3.01
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Nature Communications (publishedVersion, cc-by)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- White Rose Research Online (University of Leeds, The University of Sheffield, University of York) (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- FreiDok plus (Universitätsbibliothek Freiburg) (submittedVersion)
- Funders
- Leverhulme Trust (RPG-245)
- Royal Society (URF\R\201020)
- Research Councils UK (BB/X00645X/1, BB/R006369/1, BB/M007839/1, BB/X01097X/1)
- Lister Institute of Preventive Medicine
- Directorate for Biological Sciences
- Biotechnology and Biological Sciences Research Council (BB/X006506/1, BB/R006369/1, BB/X00645X/1, BB/M007839/1, BB/X01097X/1, BBS/E/JI/230004B)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Keywords
- chromosome segregation, archaea, Sega, chromosome organization, chromosome condensation, DNA looping, nucleoid, Sulfolobales, cell division
- MeSH terms
- Archaea, Microscopy, Atomic Force, DNA, Archaeal, Archaeal Proteins, Chromosomes, Archaeal, Chromosome Segregation, Genome, Archaeal
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