Transcription factors instruct DNA methylation patterns in plant reproductive tissues
Open accessPreprint — published as 10.1038/s41556-025-01808-5
Abstract
DNA methylation is maintained by forming self-reinforcing connections with other repressive chromatin modifications, resulting in stably silenced genes and transposons. However, these mechanisms fail to explain how new methylation patterns are generated. In Arabidopsis, CLASSY3 (CLSY3) targets the RNA-directed DNA methylation (RdDM) machinery to different loci in reproductive tissues, generating distinct epigenomes via unknown mechanism(s). Here, we discovered that several different REPRODUCTIVE MERISTEM (REM) transcription factors are required for methylation at CLSY3 targets specific to male or female reproductive tissues. We designate these factors as REM INSTRUCT METHYLATION (RIMs) and demonstrate that disruption of their DNA binding domains, or the motifs they recognize, blocks RdDM. These findings not only reveal RIMs as the first sex-specific RdDM proteins but also establish a critical role for genetic information in targeting DNA methylation. This novel mode of targeting expands our understanding of how methylation is regulated to include inputs from both genetic and epigenetic information.
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- Authors
- Xu, G., Chen, Y., Wang, F., Li, E., & Law, J. A.
- Affiliations
- Salk Institute for Biological Studies (US)
- Corresponding author
- Law, Julie A.
- Venue
- Nature Cell Biology
- Publisher
- openRxiv
- Published
- 2025-02-23
- Volume
- 27
- Issue
- 12
- Pages
- 2116-2127
- ISSN
- 1465-7392 (ISSN-L)
- PMC
- PMC12717013
- OpenAlex
- W4416458299
- Cited by
- 17
- References
- 50
- Citations by year
- 2026: 13 · 2025: 4
- FWCI
- 5.11
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- cc-by-nc-nd
- www.biorxiv.org/about/FAQ#license (vor)
- Locations
- Nature Cell Biology (publishedVersion, cc-by-nc-nd)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
- PubMed (publishedVersion)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed Central (submittedVersion)
- Funders
- U.S. Department of Health and Human Services (GM112966)
- National Institute on Aging (P30AG068635)
- National Institute of General Medical Sciences (GM112966, R01GM112966)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Chromosomal and Genetic Variations
- Keywords
- DNA methylation, transcription factors, RNA-directed DNA methylation, anther, ovule, reproductive tissues, Arabidopsis thaliana, epigenetic regulation, gene silencing, transposon silencing
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