The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Structure of the conjugation surface exclusion protein TraT
Open accessPreprint — published as 10.1038/s42003-025-09102-8 · Paper page
Abstract
Conjugal transfer of plasmids between bacteria is a major route for the spread of antimicrobial resistance. Many conjugative plasmids encode exclusion systems that inhibit redundant conjugation. In incompatibility group F (IncF) plasmids surface exclusion is mediated by the outer membrane protein TraT. Here we report the cryoEM structure of the TraT exclusion protein complex from the canonical F plasmid of Escherichia coli . TraT is a hollow homodecamer shaped like a chef’s hat. In contrast to most outer membrane proteins, TraT spans the outer membrane using transmembrane helices. We use a novel microscopy-based conjugation assay to probe the effects of directed mutagenesis on TraT. Our analysis provides no support for the idea that TraT has specific interactions with partner proteins. Instead, we infer that TraT is most likely to function by physical interference with conjugation.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Highly accurate protein structure prediction with AlphaFold
Jumper, J., Evans, R., Pritzel, A., Green, T., Figurnov, M., Ronneberger, O., Tunyasuvunakool, K., Bates, R., Žídek, A., Potapenko, A., et al. (2021) · Nature · doi:10.1038/s41586-021-03819-2 · Cited by 43,764
Flagged first, then by citations
Research works printed but not linked: 66
- 10.1016/j.mib.2024.102449 · DOI
- 10.1038/158558a0 · DOI
- 10.1038/s41579-023-00926-x · DOI
- 10.1093/genetics/37.6.720 · DOI
- 10.3390/genes11111239 · DOI
- 10.1016/j.plasmid.2013.03.010 · DOI
- 10.1128/ecosalplus.ESP-0003-2018 · DOI
- 10.1038/s41579-023-00974-3 · DOI
- 10.1038/s41586-022-04859-y · DOI
- 10.1073/pnas.1904428116 · DOI
- 10.1016/j.cell.2016.08.025 · DOI
- 10.1073/pnas.0806786105 · DOI
- 10.1128/jb.172.12.7263-7264.1990 · DOI
- 10.1073/pnas.2310842120 · DOI
- 10.1016/j.plasmid.2008.03.002 · DOI
- 10.1101/2025.04.18.649494 · DOI
- 10.1128/MMBR.33.2.210-263.1969 · DOI
- 10.1073/pnas.74.11.5104 · DOI
- 10.1016/0022-2836(80)90065-0 · DOI
- 10.1073/pnas.76.10.4837 · DOI
- 10.1016/S0021-9258(18)91011-2 · DOI
- 10.1016/0022-2836(87)90452-9 · DOI
- 10.1128/JB.142.1.285-294.1980 · DOI
- 10.1007/BF00332623 · DOI
- 10.1038/s42003-022-03587-3 · DOI
- 10.1111/j.1365-2958.1990.tb00705.x · DOI
- 10.1111/j.1365-2958.1994.tb00486.x · DOI
- 10.1111/j.1365-2958.1992.tb01462.x · DOI
- 10.1128/iai.28.2.359-367.1980 · DOI
- 10.1128/iai.35.2.654-659.1982 · DOI
- 10.4049/jimmunol.148.3.827 · DOI
- 10.4049/jimmunol.125.4.1494 · DOI
- 10.1128/jb.151.2.819-827.1982 · DOI
- 10.1128/iai.46.3.740-746.1984 · DOI
- 10.1016/0014-5793(86)80905-X · DOI
- 10.1038/s41467-025-55834-w · DOI
- 10.1073/pnas.1402746111 · DOI
- 10.1099/mic.0.2007/010462-0 · DOI
- 10.1016/S0092-8674(01)80007-6 · DOI
- 10.1242/jcs.115.6.1213 · DOI
- 10.1038/nature05267 · DOI
- 10.1038/s41586-022-04859-y · DOI
- 10.1016/j.plasmid.2008.03.002 · DOI
- 10.1017/S0016672300015093 · DOI
- 10.1038/s41564-022-01146-4 · DOI
- 10.1093/nar/gkad678 · DOI
- 10.1128/JB.180.16.4036-4043.1998 · DOI
- 10.1016/j.sbi.2022.102401 · DOI
- 10.1016/0147-619X(80)90068-2 · DOI
- 10.1128/JB.129.3.1613-1622.1977 · DOI
- 10.1073/pnas.120163297 · DOI
- 10.1038/s41396-023-01401-4 · DOI
- 10.1128/JB.184.16.4573-4581.2002 · DOI
- 10.1006/jmbi.1996.0399 · DOI
- 1951, Studies on lysogenesis I: the mode of phage liberation by lysogenic Escherichia coli, Journal of bacteriology, 62, 293, 10.1128/jb.62.3.293-300.1951 · DOI
- 10.1016/j.yjsbx.2020.100040 · DOI
- 10.1038/s41592-020-00990-8 · DOI
- 10.1002/pro.3330 · DOI · Paper page
- 10.1002/pro.3943 · DOI
- 10.1107/S2059798318006551 · DOI
- 10.1038/s41592-022-01639-4 · DOI
- 10.1038/nprot.2017.024 · DOI · Paper page
- 10.1093/bioinformatics/19.1.163 · DOI
- 10.1093/bioinformatics/btu739 · DOI · Paper page
- 10.1093/nar/gkr703 · DOI
- 10.1093/nar/16.22.10881 · DOI
Metadata
- Authors
- Chen, N., Bukys, A., Lundgren, C. A. K., Deme, J. C., El Sayyed, H., Kapanidis, A. N., Lea, S. M., & Berks, B. C.
- Affiliations
- University of Oxford (GB)
- National Cancer Institute (US)
- Biological Physics Research Group, Department of Physics, University of Oxford, Oxford OX1 3PU, United Kingdom
- St. Jude Children's Research Hospital (US)
- Corresponding author
- Lea, Susan M.
- Berks, Ben C.
- ORCID
- Venue
- Communications Biology
- Publisher
- openRxiv
- Published
- 2025-05-27
- Volume
- 8
- Issue
- 1
- Pages
- 1702-1702
- ISSN
- 2399-3642 (ISSN-L)
- PMC
- PMC12258729
- OpenAlex
- W4416718874
- Cited by
- 4
- References
- 1
- Citations by year
- 2026: 4
- FWCI
- 2.35
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Communications Biology (publishedVersion, cc-by)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (submittedVersion, cc-by)
- PubMed Central (submittedVersion, cc-by)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Titular Clarendon Fund Scholarship in partnership with the Merton College Biochemistry Scholarship and Biochemistry Studentship
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Structural Biology › Advanced Electron Microscopy Techniques and Applications
- Health Sciences › Medicine › Microbiology (medical) › Antibiotic Resistance in Bacteria
- Keywords
- traT, IncF plasmids, F plasmid, Escherichia coli, outer membrane proteins, transmembrane helices, conjugation inhibition, plasmid conjugation, antimicrobial resistance dissemination, cryo-EM structure
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
