The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
The Desmoglein 2 interactome in primary neonatal cardiomyocytes
Open accessPreprint — published as 10.1242/jcs.264213 · Paper page
Abstract
Mechanical coupling and chemical communication between cardiomyocytes are facilitated through a specialized adhesive structure known as the intercalated disc (ICD). The ICD is essential for heart organization and contraction. Yet, the network of adhesion, adaptor, and signaling proteins that form the ICD remains poorly defined. Here, we combined proximity labeling and quantitative mass spectrometry to identify proteins associated with the desmosomal cadherin, Desmoglein 2 (DSG2), in cultured neonatal cardiomyocytes. We identified over 300 proteins in the DSG2 interactome; half of which are shared with the N-cadherin (CDH2) interactome in cardiomyocytes. Proteins unique to DSG2 include the gap junction protein connexin 43 and the plakin family of cytolinker proteins. Comparison of the cardiomyocyte DSG2 interactome with the interactomes of desmosomal proteins from epithelia revealed only a small number of shared proteins. In cardiomyocytes, plakoglobin (JUP) and plakophilin 2 (PKP2) were the most abundant shared proteins between the DSG2 and CDH2 interactomes. PKP2 is a dynamic protein whose membrane recruitment in cardiomyocytes is tension-dependent. Our analysis of the DSG2 interactome provides a critical new dimension to the proteomic atlas of the essential molecular complexes required for cardiomyocyte adhesion.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Global quantification of mammalian gene expression control
Schwanhäusser, B., Busse, D., Li, N., Dittmar, G., Schuchhardt, J., Wolf, J., Chen, W., & Selbach, M. (2011) · Nature · doi:10.1038/nature10098 · Cited by 6,566
Flagged first, then by citations
Research works printed but not linked: 77
- 10.1016/j.jmb.2013.07.035 · DOI
- 10.1016/S0955-0674(00)00258-1 · DOI
- Baddam, S.R. , P.T. Arsenovic , V. Narayanan , N.R. Duggan , C.R. Mayer , S.T. Newman , D.A. Abutaleb , A. Mohan , A.P. Kowalczyk , and D.E. Conway . 2018. The Desmosomal Cadherin Desmoglein-2 Experiences Mechanical Tension as Demonstrated by a FRET-Based Tension Biosensor Expressed in Living Cells. Cells. 7. · DOI
- 10.1091/mbc.E19-09-0542 · DOI
- Bartle, E.I. , T.C. Rao , R.R. Beggs , W.F. Dean , T.M. Urner , A.P. Kowalczyk , and A.L. Mattheyses . 2020. Protein exchange is reduced in calcium-independent epithelial junctions. J Cell Biol. 219. · DOI
- 10.1016/j.ceb.2009.07.002 · DOI
- 10.1080/21688370.2021.2017225 · DOI
- Bharathan, N.K. , A.L. Mattheyses , and A.P. Kowalczyk . 2024. The desmosome comes into focus. J Cell Biol. 223. · DOI
- 10.1038/jid.2009.390 · DOI
- 10.1083/jcb.200303036 · DOI
- 10.1016/j.ejcb.2006.02.009 · DOI
- 10.1038/nbt.4201 · DOI · Paper page
- Broussard, J.A. , A. Jaiganesh , H. Zarkoob , D.E. Conway , A.R. Dunn , H.D. Espinosa , P.A. Janmey , and K.J. Green . 2020. Scaling up single-cell mechanics to multicellular tissues - the role of the intermediate filament-desmosome network. J Cell Sci. 133. · DOI
- 10.1038/s41580-023-00688-7 · DOI · Paper page
- 10.1074/jbc.M108765200 · DOI
- 10.1074/jbc.M109.047928 · DOI
- 10.1073/pnas.1203906109 · DOI
- 10.1038/nbt.1511 · DOI · Paper page
- 10.1038/ejhg.2010.19 · DOI
- 10.1161/CIRCRESAHA.110.223412 · DOI
- Ehler, E. , T. Moore-Morris , and S. Lange . 2013. Isolation and culture of neonatal mouse cardiomyocytes. J Vis Exp. · DOI
- 10.1249/MSS.0000000000003464 · DOI
- 10.1016/j.ejcb.2005.11.003 · DOI
- 10.1016/j.ejcb.2006.11.003 · DOI
- 10.1016/j.bpj.2022.02.023 · DOI
- 10.1016/j.mcpro.2024.100735 · DOI
- Fulle, J.B. , H. Huppert , D. Liebl , J. Liu , R. Alves de Almeida , B. Yanes , G.D. Wright , E.B. Lane , D.R. Garrod , and C. Ballestrem . 2021. Desmosome dualism - most of the junction is stable, but a plakophilin moiety is persistently dynamic. J Cell Sci. 134. · DOI
- 10.1242/jcs.004713 · DOI
- Guo, Z. , L.J. Neilson , H. Zhong , P.S. Murray , S. Zanivan , and R. Zaidel-Bar . 2014. E-cadherin interactome complexity and robustness resolved by quantitative proteomics. Science signaling. 7:rs7. · DOI
- 10.3109/15419061.2013.855204 · DOI
- 10.1073/pnas.1606272113 · DOI
- 10.3109/15419061.2013.876017 · DOI
- 10.1073/pnas.95.5.2509 · DOI
- 10.1016/j.devcel.2022.11.010 · DOI
- 10.1016/j.jbc.2021.100582 · DOI
- 10.1186/1471-2164-9-488 · DOI
- 10.1016/j.jmb.2008.12.054 · DOI
- 10.1161/CIRCGENETICS.114.000974 · DOI
- 10.1007/s00441-011-1322-3 · DOI
- 10.1091/mbc.E15-12-0844 · DOI
- 10.1016/B978-0-12-394311-8.00005-4 · DOI · Paper page
- 10.1007/s00395-011-0175-y · DOI
- Le, S. , M. Yu , and J. Yan . 2019. Direct single-molecule quantification reveals unexpectedly high mechanical stability of vinculin-talin/alpha-catenin linkages. Sci Adv. 5:eaav2720. · DOI
- 2016, Proximity-Dependent Biotinylation for Identification of Interacting Proteins, Current protocols in cell biology / editorial board, Juan S. Bonifacino, 73, 17 19 11
- 10.1016/S0962-8924(01)02180-8 · DOI
- 10.1242/jcs.098640 · DOI
- 10.3389/fphys.2020.612927 · DOI
- Li, Y. , C.D. Merkel , X. Zeng , J.A. Heier , P.S. Cantrell , M. Sun , D.B. Stolz , S.C. Watkins , N.A. Yates , and A.V. Kwiatkowski . 2019. The N-cadherin interactome in primary cardiomyocytes as defined using quantitative proximity proteomics. J Cell Sci. 132. · DOI · Paper page
- 10.1038/nprot.2007.135 · DOI
- Maki, K. , S.W. Han , Y. Hirano , S. Yonemura , T. Hakoshima , and T. Adachi . 2018. Real-time TIRF observation of vinculin recruitment to stretched alpha-catenin by AFM. Sci Rep-Uk. 8. · DOI
- 10.1091/mbc.E19-04-0216 · DOI
- 10.1016/j.bpj.2012.02.029 · DOI
- 10.1016/j.tcb.2013.06.004 · DOI
- 10.1152/physrev.00021.2022 · DOI
- 10.1098/rsob.240208 · DOI
- 10.1091/mbc.E19-02-0102 · DOI
- 10.1016/S0021-9258(18)99244-6 · DOI
- 10.1002/pro.5217 · DOI
- 10.1084/jem.20090641 · DOI · Paper page
- 10.1007/s12551-020-00737-x · DOI
- Rubsam, M. , J.A. Broussard , S.A. Wickstrom , O. Nekrasova , K.J. Green , and C.M. Niessen . 2018. Adherens Junctions and Desmosomes Coordinate Mechanics and Signaling to Orchestrate Tissue Morphogenesis and Function: An Evolutionary Perspective. Cold Spring Harbor perspectives in biology. 10. · DOI
- 10.1091/mbc.E17-04-0231 · DOI
- Shafraz, O. , M. Rubsam , S.N. Stahley , A.L. Caldara , A.P. Kowalczyk , C.M. Niessen , and S. Sivasankar . 2018. E-cadherin binds to desmoglein to facilitate desmosome assembly. eLife. 7. · DOI
- 10.1073/pnas.2010209117 · DOI
- 10.1021/bi047881x · DOI
- 10.1073/pnas.91.23.10790 · DOI
- 10.1038/nprot.2016.136 · DOI
- 10.1093/nar/gkq1020 · DOI
- 10.1093/eurheartj/ehs373 · DOI
- 10.1242/jcs.140475 · DOI
- 10.1016/j.nmd.2014.09.011 · DOI
- 10.3109/15419061.2014.908853 · DOI
- 10.1038/ncomms5525 · DOI
- 10.1111/joa.13634 · DOI
- 10.1038/ncb2055 · DOI · Paper page
- 10.1016/j.biopha.2023.115448 · DOI
- 10.1093/cvr/cvy061 · DOI
Metadata
- Authors
- Li, Y., Campbell, A. P., Balasubramanian, S., Zeng, X., Porter, E., Cantrell, P. S., Sun, M., Mattheyses, A. S., & Kwiatkowski, A. V.
- Affiliations
- University of Pittsburgh (US)
- Department of Cell, Developmental, and Integrative Biology, The University of Alabama at Birmingham, Birmingham, AL, USA
- Corresponding author
- Kwiatkowski, Adam V.
- Venue
- Journal of Cell Science
- Publisher
- openRxiv
- Published
- 2025-06-10
- Volume
- 139
- Issue
- 3
- ISSN
- 0021-9533 (ISSN-L)
- PMC
- PMC12912264
- OpenAlex
- W7128019313
- Cited by
- 2
- References
- 1
- Citations by year
- 2026: 1 · 2025: 1
- FWCI
- 4.53
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Cell Science (publishedVersion, cc-by)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- PubMed Central (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (submittedVersion, cc-by-nc-nd)
- PubMed Central (submittedVersion, cc-by-nc-nd)
- Funders
- American Heart Association
- University of Pittsburgh
- National Institutes of Health
- National Heart, Lung, and Blood Institute (R01HL127711)
- National Institute of Arthritis and Musculoskeletal and Skin Diseases (R01AR072697)
- Topics
- Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Skin and Cellular Biology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Biotin and Related Studies
- Keywords
- desmoglein 2, desmosomes, intercalated discs, cardiomyocyte adhesion, protein-protein interaction network, N-cadherin, connexin 43, plakoglobin, plakophilin-2, desmosomal cadherins, mechanical tension, membrane recruitment, neonatal cardiomyocytes
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
