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Diversification of functional requirements for proteolysis of Auxin Response Factors
Open accessPreprint — published as 10.1038/s41467-026-70440-0 · Paper page
Abstract
Auxin signaling through the Nuclear Auxin Pathway is essential for plant development and is mediated by competing A-class and B-class Auxin Response Factor (ARF) transcription factors. Recently, proteasomal ARF degradation through a degradation signal embedded within the DNA-Binding Domain, was identified as a key component of auxin response. Here, we investigate the structural requirements and biological relevance of ARF degradation in the bryophyte Marchantia polymorpha . We identify a critical residue for proteolysis of the repressive, B-class MpARF2, and find it to be functionally conserved in degradation of the activating, A-class MpARF1. Unlike MpARF2, however, impaired MpARF1 degradation had little effect on auxin response and development, suggesting differential integration in biological function. We find that MpARF2 degradation occurs across all developmental stages of the life cycle, and is required for MpARF2 function during development. Our findings reveal a degradation mechanism targeting A- and B-class ARFs which shares the same origin, but has evolved along unique evolutionary trajectories.
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Jumper, J., Evans, R., Pritzel, A., Green, T., Figurnov, M., Ronneberger, O., Tunyasuvunakool, K., Bates, R., Žídek, A., Potapenko, A., et al. (2021) · Nature · doi:10.1038/s41586-021-03819-2 · Cited by 43,764
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Metadata
- Authors
- de Roij, M., Heijdra, E., Nishihama, R., Borst, J. W., & Weijers, D.
- Affiliations
- Wageningen University & Research (NL)
- Tokyo University of Science (JP)
- Corresponding author
- Weijers, Dolf
- Venue
- Nature Communications
- Publisher
- openRxiv
- Published
- 2025-09-04
- Volume
- 17
- Issue
- 1
- ISSN
- 2041-1723 (ISSN-L)
- PMC
- PMC13128892
- OpenAlex
- W7135178000
- Cited by
- 3
- References
- 1
- Citations by year
- 2026: 3
- FWCI
- 11.10
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Nature Communications (publishedVersion, cc-by-nc-nd)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc)
- PubMed (publishedVersion)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Socio-Environmental Systems Modeling (submittedVersion, cc-by-nc-nd)
- Funders
- Netherlands Organization for Sciencetific Research (OCENW.M20.031)
- Human Frontiers Science Program (RGP0015/2022)
- Japan Society for the Promotion of Science (JP18H04836, JP20H04884, JP22H04733)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Light effects on plants
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Parasitism and Resistance
- Keywords
- auxin response factor, proteasomal degradation, auxin signaling, Marchantia polymorpha, liverworts, bryophytes, DNA-binding domain, plant development, transcriptional repression
- MeSH terms
- Amino Acid Sequence, Indoleacetic Acids, Plant Proteins, Transcription Factors, Signal Transduction, Gene Expression Regulation, Plant, Marchantia, Proteolysis
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