Methods Matter -- Standard Production Platforms for Recombinant AAV Produce Chemically and Functionally Distinct Vectors

Rumachik, Neil G.; Malaker, Stacy A.; Poweleit, Nicole; Maynard, Lucy H.; Adams, Christopher M.; Leib, Ryan D.; Cirolia, Giana; Thomas, Dennis; Stamnes, Susan; Holt, Kathleen; Sinn, Patrick; May, Andrew P.; Paulk, Nicole K.

doi:10.1101/640169

Methods Matter -- Standard Production Platforms for Recombinant AAV Produce Chemically and Functionally Distinct Vectors

Different manufacturing approaches have been used in the production of recombinant adeno-associated virus (rAAV). The two leading approaches are transiently transfected human HEK293 cells and live baculovirus infection of Sf9 insect cells. Unexplained differences in vector performance have been s…