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PreprintCited by 3Chromosomal and Genetic VariationsGenomics and Chromatin DynamicsEpigenetics and DNA Methylation

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Adaptive evolution targets a piRNA precursor transcription network

Open access
Parhad, Swapnil S., Yu, Tianxiong, Zhang, Gen, Rice, Nicholas P., Weng, Zhiping, Theurkauf, William E.·2019·bioRxiv (Cold Spring Harbor Laboratory)
DOI 10.1101/678227

Preprint — published as 10.1016/j.celrep.2020.01.109 · Paper page

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Abstract

SUMMARY: In Drosophila , transposon-silencing piRNAs are derived from heterochromatic clusters and a subset of euchromatic transposon insertions, which are transcribed from internal non-canonical initiation sites and flanking canonical promoters. Rhino binds to Deadlock, which recruits TRF2 to promote non-canonical transcription of these loci. Cuff co-localizes with Rhino and Del. The role of Cuff is less well understood, but the cuff gene shows hallmarks of adaptive evolution, which frequently targets functional interactions within host defense systems. We show that Drosophila simulans cuff is a dominant negative allele when expressed in Drosophila melanogaster , where it traps Deadlock, TRF2 and the transcriptional co-repressor CtBP in stable nuclear complexes. Cuff promotes Rhino and Deadlock localization, driving non-canonical transcription. CtBP, by contrast, suppresses canonical cluster and transposon transcription, which interferes with downstream non-canonical transcription and piRNA production. Cuff, TRF2 and CtBP thus form a network that balances canonical and non-canonical piRNA precursor transcription.

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Metadata

Authors
Parhad, S. S., Yu, T., Zhang, G., Rice, N. P., Weng, Z., & Theurkauf, W. E.
Affiliations
  • University of Massachusetts Chan Medical School (US)
  • Tongji University (CN)
Corresponding author
  • Weng, Zhiping
  • Theurkauf, William E.
ORCID
Venue
bioRxiv (Cold Spring Harbor Laboratory)
Publisher
openRxiv
Published
2019-06-21
DOI
10.1101/678227
OpenAlex
W2949174241
Cited by
3
References
2
Citations by year
2024: 1 · 2022: 1 · 2020: 1
Type
posted content
Language
en
Open access
Licence
cc-by-nc-nd
Locations
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • eScholarship@UMassChan (submittedVersion, other-oa)
Funders
  • University of California, San Diego
  • National Institute of Child Health and Human Development
  • Eunice Kennedy Shriver National Institute of Child Health and Human Development (P01HD078253, R01HD049116)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Chromosomal and Genetic Variations
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
Keywords
adaptive evolution, Drosophila simulans, Drosophila melanogaster, transposon silencing, rhinoceros, deadlock, TRF2, cuffs, CtBP, heterochromatin, dominant-negative allele, piRNA biogenesis, host defense

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