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Switchgrass Rhizosphere Metabolite Chemistry Driven by Nitrogen Availability
Open accessPreprint — published as 10.1094/pbiomes-09-19-0055-fi · Paper page
Abstract
Plants and soil microorganisms interact closely in the rhizosphere where plants may exchange carbon (C) for functional benefits from the microbial community. For example, the bioenergy crop, switchgrass ( Panicum virgatum ) is thought to exchange root-exuded C for nitrogen (N) fixed by diazotrophs (free-living N-fixers). However, this interaction is not well characterized and it is not known how or if switchgrass responds to diazotrophs or their activity. To explore this question, we assessed rhizosphere metabolite chemistry of switchgrass grown in a hydroponic system under two N levels and under inoculated or uninoculated conditions. We found switchgrass root exudate chemistry to be more responsive to N availability than to diazotroph presence. Total metabolite concentrations were generally greater under high N versus low N and unaffected by inoculation. Examination of rhizosphere chemical fingerprints indicates metabolite chemistry was also driven strongly by N availability with a greater relative abundance of carbohydrates under high N and greater relative abundance of organic acids under low N. We also found evidence of changes in rhizosphere chemical fingerprints by inoculation treatment suggesting a potential for switchgrass to respond or even recruit diazotrophs. However, we found little evidence of N treatment and inoculation interaction effects which suggests switchgrass response to diazotroph presence is not mediated by N availability.
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Cites
Nitrogen fertilization challenges the climate benefit of cellulosic biofuels
Ruan, L., Bhardwaj, A. K., Hamilton, S. K., & Robertson, G. P. (2016) · Environmental Research Letters · doi:10.1088/1748-9326/11/6/064007 · Cited by 85 · cited 2×
“Switchgrass productivity is often unresponsive to fertilizer N additions, resulting in similar biomass yields with fertilizer N additions well above or below plant N demands (Parrish and Fike 2005; Ruan et al. 2016).”
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Metadata
- Authors
- Smercina, D. N., Bowsher, A. W., Evans, S. E., Friesen, M. L., Eder, E. K., Hoyt, D. W., & Tiemann, L. K.
- Affiliations
- Michigan State University (US)
- Washington State University (US)
- Pacific Northwest National Laboratory (US)
- ORCID
- Venue
- Phytobiomes Journal
- Publisher
- openRxiv
- Published
- 2019-10-16
- Volume
- 5
- Issue
- 1
- Pages
- 88-96
- ISSN
- 2471-2906 (ISSN-L)
- OpenAlex
- W3010294298
- Cited by
- 18
- References
- 1
- Citations by year
- 2026: 4 · 2024: 3 · 2023: 1 · 2022: 5 · 2021: 4 · 2020: 1
- FWCI
- 1.78
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by-nc-nd
- Locations
- Phytobiomes Journal (publishedVersion, cc-by-nc-nd)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
- OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Science Foundation (DEAC02-05CH11231, 1832042, DE-SC0018409, DEAC05–76RL01830, 76RL01830)
- U.S. Department of Energy (FC02-07ER64494, DE-AC05–76RL01830, -AC02-05CH11231, 07ER64494, DESC0014108, 76RL01830, AC05-76RL01830, DE-AC05?, 05CH11231, grid.436923.9, DE AC02 05CH11231, 436923.9, DEFC0207ER64494, DESC0018409, DEB 1832042, DE-AC02-)
- Michigan State University (DE-AC02-05CH11231, DEB 1832042)
- Joint Genome Institute (AC02-05CH11231, DE–AC02–05CH11231)
- Office of Science (DESC0014108, DESC0018409, DEAC02–05CH11231, FC02-07ER64494, AC02-05CH11231, DE-AC02, grid.436923.9, DEFC0207ER64494, DE‐AC05‐76 RL01830)
- College of Engineering, Michigan State University
- Great Lakes Bioenergy Research Center (DE‐FC02‐07ER64494, DE-SC0018409, DEB 1832042, SC0018409, DE-AC02-05CH11231)
- Biological and Environmental Research (DE-AC05-76 RL01830, 05CH11231, DE‐SC0018409, DE-AC02 − 05CH11231, AC05-76RL01830, DE-FC02-07ER64494, 76RL01830, SC0018409, DE-SC0014108, AC02-05CH11231, grid.436923.9)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant-Microbe Interactions and Immunity
- Life Sciences › Agricultural and Biological Sciences › Soil Science › Soil Carbon and Nitrogen Dynamics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Legume Nitrogen Fixing Symbiosis
- Keywords
- switchgrass, rhizosphere metabolites, nitrogen availability, root exudates, Azotobacter vinelandii, nitrogen-fixing bacteria, nitrogen fixation, plant-microbe interactions, organic acids, carbohydrates, energy crops, hydroponics
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