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mRNA capping enzyme is recruited to the transcription complex by phosphorylation of the RNA polymerase II carboxy-terminal domain
Open accessAbstract
Capping of mRNA occurs shortly after transcription initiation, preceding other mRNA processing events such as mRNA splicing and polyadenylation. To determine the mechanism of coupling between transcription and capping, we tested for a physical interaction between capping enzyme and the transcription machinery. Capping enzyme is not stably associated with basal transcription factors or the RNA polymerase II (Pol II) holoenzyme. However, capping enzyme can directly and specifically interact with the phosphorylated form of the RNA polymerase carboxy-terminal domain (CTD). This association occurs in the context of the transcription initiation complex and is blocked by the CTD–kinase inhibitor H8. Furthermore, conditional truncation mutants of the Pol II CTD are lethal when combined with a capping enzyme mutant. Our results provide in vitro and in vivo evidence that capping enzyme is recruited to the transcription complex via phosphorylation of the RNA polymerase CTD.
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Cited by
Yamaguchi, Y., Takagi, T., Wada, T., Yano, K., Furuya, A., Sugimoto, S., Hasegawa, J., & Handa, H. (1999) · Cell · doi:10.1016/s0092-8674(00)80713-8 · Cited by 841
Self-citationCoupling mRNA processing with transcription in time and space
Bentley, D. L. (2014) · Nature Reviews Genetics · doi:10.1038/nrg3662 · Cited by 828
Elongation by RNA polymerase II: the short and long of it
Sims, R. J., Belotserkovskaya, R., & Reinberg, D. (2004) · Genes & Development · doi:10.1101/gad.1235904 · Cited by 709
Temporal Regulation of RNA Polymerase II by Srb10 and Kin28 Cyclin-Dependent Kinases
Hengartner, C. J., Myer, V. E., Liao, S.-M., Wilson, C. J., Koh, S. S., & Young, R. A. (1998) · Molecular Cell · doi:10.1016/s1097-2765(00)80112-4 · Cited by 408
Li, B., Howe, L., Anderson, S., Yates, J. R., & Workman, J. L. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m212134200 · Cited by 371
Metadata
- Authors
- Cho, E.-J., Takagi, T., Moore, C. R., & Buratowski, S.
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 1997-12-15
- Volume
- 11
- Issue
- 24
- Pages
- 3319-3326
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- OpenAlex
- W2101424708
- Cited by
- 478
- References
- 0
- Type
- posted content
- Language
- en
- Open access
- diamond
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- Cho, E.-J., Takagi, T., Moore, C. R., & Buratowski, S. (1997). mRNA capping enzyme is recruited to the transcription complex by phosphorylation of the RNA polymerase II carboxy-terminal domain. Genes & Development. https://doi.org/10.1101/gad.11.24.3319
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