The DOI matches its Crossref record; 78 of the 80 references it cites were found in a registry or an index.
Tcn1p/Crz1p, a calcineurin-dependent transcription factor that differentially regulates gene expression in Saccharomyces cerevisiae
Open accessAbstract
Ca 2+ signals regulate gene expression in animal and yeast cells through mechanisms involving calcineurin, a protein phosphatase activated by binding Ca 2+ and calmodulin. Tcn1p, also named Crz1p, was identified as a transcription factor in yeast required for the calcineurin-dependent induction of PMC1, PMR1, PMR2A, and FKS2 which confer tolerance to high Ca 2+ , Mn 2+ , Na + , and cell wall damage, respectively. Tcn1p was not required for other calcineurin-dependent processes, such as inhibition of a vacuolar H + /Ca 2+ exchanger and inhibition of a pheromone-stimulated Ca 2+ uptake system, suggesting that Tcn1p functions downstream of calcineurin on a branch of the calcium signaling pathway leading to gene expression. Tcn1p contains three zinc finger motifs at its carboxyl terminus resembling the DNA-binding domains of Zif268, Swi5p, and other transcription factors. When fused to the transcription activation domain of Gal4p, the carboxy terminal domain of Tcn1p directed strong calcineurin-independent expression of PMC1–lacZ and other target genes. The amino-terminal domain of Tcn1p was found to function as a calcineurin-dependent transcription activation domain when fused to the DNA-binding domain of Gal4p. This amino-terminal domain also formed Ca 2+ -dependent and FK506-sensitive interactions with calcineurin in the yeast two-hybrid assay. These findings suggest that Tcn1p functions as a calcineurin-dependent transcription factor. Interestingly, induction of Tcn1p-dependent genes was found to be differentially controlled in response to physiological Ca 2+ signals generated by treatment with mating pheromone and high salt. We propose that different promoters are sensitive to variations in the strength of Ca 2+ signals generated by these stimuli and to effects of other signaling pathways.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The cited source says what the claim attributes to it.
In the citing paper
“In the model and fission yeasts, this phenotype has been associated to a decreased expression of the calcineurin-regulated PMC1 gene encoding the vacuolar calcium pump (Matheos et al. 1997;Hirayama et al. 2003).”
From Functional characterization of the Candida albicans CRZ1 gene encoding a calcineurin-regulated transcription factor (2005) · cites this paper as Matheos et al. 1997
In the citing paper
“Calcineurin-dependent expression of these genes is regulated by the ScCrz1/Tcn1/Hal8 transcription factor (Stathopoulos and Cyert 1997;Matheos et al. 1997;Mendizabal et al. 1998)”
From Functional characterization of the Candida albicans CRZ1 gene encoding a calcineurin-regulated transcription factor (2005) · cites this paper as Matheos et al. 1997
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Molecular Cloning: A Laboratory Manual
Sambrook, J., Fritsch, E., & Maniatis, T. (1989) · UCLA - Biblioteca de Ciencias de la Salud · Cited by 133,589
Calcium's Role in Mechanotransduction during Muscle Development
Damm, T. B., & Egli, M. (2014) · Cellular Physiology and Biochemistry · doi:10.1159/000356667 · Cited by 14,552
Sikorski, R. S., & Hieter, P. (1989) · Genetics · doi:10.1093/genetics/122.1.19 · Cited by 8,590
Sherman, F., Fink, G. R., & Hicks, J. B. (1979) · Cold Spring Harbor Laboratory eBooks · Cited by 7,430
The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases.
Wade Harper, J. (1993) · Cell · doi:10.1016/0092-8674(93)90499-g · Cited by 5,651
TRANSCRIPTION FACTORS OF THE NFAT FAMILY:Regulation and Function
Rao, A., Luo, C. H., & Hogan, P. G. (1997) · Annual Review of Immunology · doi:10.1146/annurev.immunol.15.1.707 · Cited by 2,592
The mechanism of action of cyclosporin A and FK506
Schreiber, S. L., & Crabtree, G. R. (1992) · Immunology Today · doi:10.1016/0167-5699(92)90111-j · Cited by 2,127
Zinc Finger-DNA Recognition: Crystal Structure of a Zif268-DNA Complex at 2.1 Å
Pavletich, N. P., & Pabo, C. O. (1991) · Science · doi:10.1126/science.2028256 · Cited by 2,081
Differential activation of transcription factors induced by Ca2+ response amplitude and duration
Dolmetsch, R. E., Lewis, R. S., Goodnow, C. C., & Healy, J. I. (1997) · Nature · doi:10.1038/386855a0 · Cited by 1,763
Differential activation of transcription factors induced by Ca2+ response amplitude and duration
Dolmetsch, R. E., Lewis, R. S., Goodnow, C. C., & Healy, J. I. (1997) · Nature · doi:10.1038/40912 · Cited by 1,521
Cited by
Sorted by citations
Items 301–331 of 331
Ren, X., Wang, Y., Liu, Y., Tan, Y., Ren, C., Ge, Y., & Liu, Z. (2017) · Journal of Basic Microbiology · doi:10.1002/jobm.201700277 · Cited by 2
Multiple mechanisms impact fluconazole resistance of mutant Erg11 proteins in Candida glabrataPreprint
Vu, B. G., & Moye-Rowley, S. (2021) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2021.06.23.449691 · Cited by 2
Plant responses to osmotic stress
Verbruggen, N., & MONTAGU, M. V. (1999) · Dépôt institutionnel de l'Université libre de Bruxelles (Université Libre de Bruxelles) · Cited by 2
Su, Y., Li, Y., Xiao, M., Guo, D., Perlin, D. S., Shor, E., Xu, Y., & Li, Y. (2026) · Advanced Science · doi:10.1002/advs.76369 · Cited by 2
EVALUATION OF SALT TOLERANCE IN STO TRANSFORMED ARABIDOPSIS THALIANA AND NICOTIANA TABACUM PLANTS
Selçuk, F. (2004) · Cited by 1
Biosynthetic Enzymes for (1-3)-β-Glucans, (1-3;1-6)-β-Glucans from Yeasts
Nogami, S., & Ohya, Y. (2009) · Elsevier eBooks · doi:10.1016/b978-0-12-373971-1.00020-0 · Cited by 1
Roy, A., & Tamuli, R. (2022) · Mycological Progress · doi:10.1007/s11557-022-01833-w · Cited by 1
Signalling towards cell wall synthesis in budding yeast.
Raclavský, V. (1998) · PubMed · Cited by 1
Torulaspora delbrueckii: aplicaciones tecnológicas y aislamiento de genes de respuesta a estrés.
López, H., & José, M. (2005) · Tesis Doctorals en Xarxa (Consorci de Serveis Universitaris de Catalunya)
Protein Structure–Function Paradigm in Plant Stress Tolerance
Bhatt, H., Kumar, A., & Bhavesh, N. S. (2013) · doi:10.1002/9783527675265.ch17
Chitin synthesis in response to environmental stress
Pauw, M. (2014) · SUNScholar (Stellenbosch University)
Causes and Consequences of Gene Expression Noise
Dalal, C. K. (2010) · CaltechTHESIS (California Institute of Technology) · doi:10.7907/mhsr-9875
Ruiz, A. (2006) · TDX (Tesis Doctorals en Xarxa)
Direktor, Ä., & Autenrieth, I. B. (2007)
Characterization of putative magnesium transport systems in yeast
Franklin, A. (2007) · IRL - University of Missouri, St. Louis (University of Missouri–St. Louis)
Ca2+-ATPase Genes and Related Diseases
Rao, R., & Inesi, G. (2003) · Springer eBooks · doi:10.1007/978-1-4419-9023-5_24
Calcineurin-NFAT Signaling in Cardiac Hypertrophy: In Sickness and In Health?
Wilkins, B. J. (2004) · OhioLink ETD Center (Ohio Library and Information Network)
Regulation of the Alr1 transporter by Mg supply
Lim, P. H. (2009) · IRL - University of Missouri, St. Louis (University of Missouri–St. Louis)
Mechanisms Through Which Lithocholic Acid and Caloric Restriction Delay Yeast Chronological Aging
Leonov, A. (2018) · Spectrum Research Repository (Concordia University)
Redox regulation of protein serine/threonine phosphatase calcineurin
Namgaladze, D. (2002) · KOPS (University of Konstanz)
Rogers, J. V., Yeo, A., Meleshkevich, V., Lorenzi, H., Rose, M. D., & Cohen‐Fix, O. (2026) · Genetics · doi:10.1093/genetics/iyag051
Moon, H., Park, J., Shin, S., Kim, S., Kim, J.-E., & Son, H. (2026) · Microbiology Spectrum · doi:10.1128/spectrum.01537-26
Chen, C., 謝月如;Hsieh, Y.-J., Lin, C.-H., Hsu, L.-H., & Chen, Y. (2026) · Journal of Agriculture and Food Research · doi:10.1016/j.jafr.2026.103300
Calcineurin-mediated regulation of macrophage plasticity drives anti-tumor activity
Yang, R., Chen, Y., Liu, J., Hou, R., Chao, Y., Wang, Y., Wang, N., Tan, B., Zhang, N., Liu, M., et al. (2026) · Molecular Therapy Advances · doi:10.1016/j.omta.2026.201853
Biochemische und genetische Untersuchungen zu Calcineurin aus Dictyostelium
Kessen, U. A. (2000)
Structure of Calcineurin and Its Complex with Immunophilins
Klee, C. B. (2000) · Springer eBooks · doi:10.1007/978-3-642-58306-3_6
The role of calcium in Saccharomyces sp. response to ethanol stress
Duarte, S. O. D. (2011) · Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT)
Metadata
- Authors
- Matheos, D. P., Kingsbury, T. J., Ahsan, U. S., & Cunningham, K. W.
- Affiliations
- Johns Hopkins University (US)
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 1997-12-15
- Volume
- 11
- Issue
- 24
- Pages
- 3445-3458
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- PMC
- PMC316804
- OpenAlex
- W2080565111
- Cited by
- 332
- References
- 80
- Citations by year
- 2026: 5 · 2025: 5 · 2024: 4 · 2023: 6 · 2022: 9 · 2021: 8 · 2020: 3 · 2019: 8 · 2018: 5 · 2017: 10 · 2016: 11 · 2015: 11 · 2014: 10 · 2013: 7 · 2012: 9
- Type
- posted content
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Genes & Development (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- HighWire Press Open Archive (submittedVersion)
- Funders
- March of Dimes Foundation
- Chicago Community Trust
- Searle Scholars Program
- National Institutes of Health
- National Institute of General Medical Sciences (GM53082, R01GM053082)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Signaling Pathways in Disease
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- calcineurin, calcium signaling, transcription factors, zinc finger, transactivation domain, PMR1, FKS2, high salt stress, mating pheromone, Saccharomyces cerevisiae
- MeSH terms
- Calcium-Transporting ATPases, Amino Acid Sequence, beta-Galactosidase, Binding Sites, Calcium, DNA-Binding Proteins, Fungal Proteins, Glucosyltransferases, Membrane Proteins, Molecular Sequence Data, Pheromones, Saccharomyces cerevisiae, Transcription Factors, Trans-Activators, Gene Expression Regulation, Fungal, Genes, Reporter, Calcineurin, Saccharomyces cerevisiae Proteins, Plasma Membrane Calcium-Transporting ATPases
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
