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A prokaryotic-like mode of cytoplasmic eukaryotic ribosome binding to the initiation codon during internal translation initation of hepatitis C and classical swine fever virus RNAs
Open accessAbstract
Initiation of translation of hepatitis C virus and classical swine fever virus mRNAs results from internal ribosomal entry. We reconstituted internal ribosomal entry in vitro from purified translation components and monitored assembly of 48S ribosomal preinitiation complexes by toe-printing. Ribosomal subunits (40S) formed stable binary complexes on both mRNAs. The complex structure of these RNAs determined the correct positioning of the initiation codon in the ribosomal “P” site in binary complexes. Ribosomal binding and positioning on these mRNAs did not require the initiation factors eIF3, eIF4A, eIF4B, and eIF4F and translation of these mRNAs was not inhibited by a trans-dominant eIF4A mutant. Addition of Met–tRNA iMet, eIF2, and GTP to these binary ribosomal complexes resulted in formation of 48S preinitiation complexes. The striking similarities between this eukaryotic initiation mechanism and the mechanism of translation initiation in prokaryotes are discussed.
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Citations
Cited by
The mechanism of eukaryotic translation initiation and principles of its regulation
Jackson, R. J., Hellen, C. U. T., & Pestova, T. V. (2010) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2838 · Cited by 2,759
Self-citationeIF4 Initiation Factors: Effectors of mRNA Recruitment to Ribosomes and Regulators of Translation
Gingras, A.-C., Raught, B., & Sonenberg, N. (1999) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.68.1.913 · Cited by 2,121
Mechanism of ribosome recruitment by hepatitis C IRES RNA
KIEFT, J. S., ZHOU, K., JUBIN, R., & DOUDNA, J. A. (2001) · RNA · doi:10.1017/s1355838201001790 · Cited by 369
Antitumor Activity and Mechanism of Action of the Cyclopenta[b]benzofuran, Silvestrol
Cencic, R., Carrier, M., Galicia-Vázquez, G., Bordeleau, M.-E., Sukarieh, R., Bourdeau, A., Brem, B., Teodoro, J. G., Greger, H., Tremblay, M. L., et al. (2009) · PLoS ONE · doi:10.1371/journal.pone.0005223 · Cited by 302
The Pathway of HCV IRES-Mediated Translation Initiation
Otto, G. A., & Puglisi, J. D. (2004) · Cell · doi:10.1016/j.cell.2004.09.038 · Cited by 296
Metadata
- Authors
- Pestova, T. V., Shatsky, I. N., Fletcher, S. P., Jackson, R. J., & Hellen, C. U. T.
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 1998-01-01
- Volume
- 12
- Issue
- 1
- Pages
- 67-83
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- OpenAlex
- W2104947461
- Cited by
- 729
- References
- 0
- Type
- posted content
- Language
- en
- Open access
- diamond
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- CoChat. (2026). Citation check: A prokaryotic-like mode of cytoplasmic eukaryotic ribosome binding to the initiation codon during internal translation initation of hepatitis C and classical swine fever virus RNAs. https://app.cochat.ai/papers/10.1101/gad.12.1.67
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- Pestova, T. V., Shatsky, I. N., Fletcher, S. P., Jackson, R. J., & Hellen, C. U. T. (1998). A prokaryotic-like mode of cytoplasmic eukaryotic ribosome binding to the initiation codon during internal translation initation of hepatitis C and classical swine fever virus RNAs. Genes & Development. https://doi.org/10.1101/gad.12.1.67
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