Phosphorylation of mammalian Sgo2 by Aurora B recruits PP2A and MCAK to centromeres
Open accessAbstract
Shugoshin (Sgo) is a conserved centromeric protein. Mammalian Sgo1 collaborates with protein phosphatase 2A (PP2A) to protect mitotic cohesin from the prophase dissociation pathway. Although another shugoshin-like protein, Sgo2, is required for the centromeric protection of cohesion in germ cells, its precise molecular function remains largely elusive. We demonstrate that hSgo2 plays a dual role in chromosome congression and centromeric protection of cohesion in HeLa cells, while the latter function is exposed only in perturbed mitosis. These functions partly overlap with those of Aurora B, a kinase setting faithful chromosome segregation. Accordingly, we identified the phosphorylation of hSgo2 by Aurora B at the N-terminal coiled-coil region and the middle region, and showed that these phosphorylations separately promote binding of hSgo2 to PP2A and MCAK, factors required for centromeric protection and chromosome congression, respectively. Furthermore, these phosphorylations are essential for localizing PP2A and MCAK to centromeres. This mechanism seems applicable to germ cells as well. Thus, our study identifies Sgo2 as a hitherto unknown crucial cellular substrate of Aurora B in mammalian cells.
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Aurora B indirectly facilitates MCAK association with centromeres by phosphorylation of Sgo2, which then interacts with MCAK and targets it to centromeres.
“Aurora B also indirectly facilitates MCAK association with centromeres by phosphorylation of Sgo2, which then interacts with MCAK and targets it to centromeres [31].”
From Regulatory mechanisms that control mitotic kinesins (2015) · cites this paper as [31]
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“Aurora B also indirectly facilitates MCAK association with centromeres by phosphorylation of Sgo2, which then interacts with MCAK and targets it to centromeres [31].”
Background
- Authors
- Tanno, Y., Kitajima, T. S., Honda, T., Ando, Y., Ishiguro, K.-I., & Watanabe, Y.
- Affiliations
- Institute for Quantitative Biosciences, The University of Tokyo (JP)
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2010-10-01
- Volume
- 24
- Issue
- 19
- Pages
- 2169-2179
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- PMC
- PMC2947769
- OpenAlex
- W2105788580
- Cited by
- 139
- References
- 48
- Citations by year
- 2026: 4 · 2025: 3 · 2024: 1 · 2023: 2 · 2022: 11 · 2021: 8 · 2020: 10 · 2019: 10 · 2018: 6 · 2017: 12 · 2016: 11 · 2015: 13 · 2014: 8 · 2013: 10 · 2012: 19
- Type
- posted content
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- other-oa
- Locations
- Genes & Development (publishedVersion, other-oa)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Education, Culture, Sports, Science and Technology
- Japan Society for the Promotion of Science (21000010)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Keywords
- shugoshin, Aurora B kinase, protein phosphorylation, protein phosphatase 2A, MCAK, centromere, chromosome congression, centromeric cohesion, coiled-coil domain, germ cells, mitosis
- MeSH terms
- Cells, Cultured, Centromere, HeLa Cells, Humans, Phosphorylation, Kinesins, Protein Serine-Threonine Kinases, Cell Cycle Proteins, Protein Transport, Protein Phosphatase 2, Aurora Kinase B, Aurora Kinases
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